Antibodies to mutated citrullinated vimentin and antibodies to cyclic citrullinated peptides in juvenile idiopathic arthritis

Abstract

Background: The goal of the study was to assess the presence of antibodies to mutated citrullinated vimentin (anti-MCV) and cyclic citrullinated peptides (anti-CCP) in patients with juvenile idiopathic arthritis (JIA) compared with patients with other juvenile onset rheumatic diseases. Methods: The study included 56 patients who fulfilled the International League of Associations for Rheumatology (ILAR) classification criteria for JIA, and 17 control patients with other juvenile onset rheumatic diseases. Data on six core outcome variables and the Sharp score were collected for patients with JIA. Sera and synovial fluid, if available, were tested for anti-CCP and anti-MCV antibodies using a commercial enzyme-linked immunosorbent assay (ELISA). Results: Anti-MCV antibodies were positive in 3/56 (5.4%) and anti-CCP in 1/56 (1.8%) of patients with JIA. Two out of three anti-MCV positive patients (one of them also anti-CCP positive) were found to be rheumatoid factor (RF)-positive with polyarticular disease. Within the control group, anti-MCV was positive in 4/17 (23.5%) patients, while anti-CCP positivity was not observed. No correlation between anti-MCV with anti-CCP antibody levels was found for any of the six core outcome variables or for the adapted Sharp score. Conclusions: Our results show that antibodies targeting citrullinated proteins are not a useful diagnostic marker for JIA, but can indicate severe patterns of disease in JIA. Clin Chem Lab Med 2009;47:1525-30.

Anti-type II collagen antibodies, anti-CCP, IgA RF and IgM RF are associated with joint damage, assessed eight years after onset of juvenile idiopathic arthritis (JIA)

“…Occurrence of autoantibodies was rare in our study, much like in the majority of previous studies on JIA [9,21-23]. As in adult RA, occurrence of IgM RF in JIA has been shown to correlate with joint damage or joint space narrowing [5,17,22,24-26].…”

Evidence of fibrinogen as a target of citrullination in IgM rheumatoid factor-positive polyarticular juvenile idiopathic arthritis

“…Other studies have examined the significance of citrullinated fibrinogen [13-19], citrullinated α-enolase [13,20,21], citC1 III [13,22-24], and citrullinated vimentin [13,25-30] in RA and experimental arthritis. To date, only two studies on citrullinated vimentin has documented the identity and evaluated the significance of citrullinated proteins in a JIA population [31,32]. …”

Diagnostic performance of anti-citrullinated protein/peptide antibodies in juvenile idiopathic arthritis

“…Citrullinated vimentin has been shown to be the target for the previously described RA-specific Sa antibodies (Vossenaar et al, 2004). The prevalence of anti-MCV has been reported recently to vary between 5.4 and 10.5%, and it was also found in disease controls (Kuna et al, 2009;Morbach et al, 2010;Gilliam et al, 2013). The prevalence of anti-MCV in our JIA patient group was 28.4%, which was higher than that of any other ACPA; however, 3 of the 49 healthy controls were found to be positive for anti-MCV, and 23.6% of disease controls also showed positive reactivity, very similar to the results reported by Kuna.…”

“…Meanwhile, each ACPA had a different prevalence among the JIA subtypes, mainly because of the antigens used. Anti-CCP has been extensively studied in JIA, and the sensitivity of anti-CCP has been reported to be between 1.8 and 27.9% (Machado et al, 2005;Brunner and Sitzmann, 2006;Gilliam et al, 2008;Habib et al, 2008;Kuna et al, 2009). In the present study, anti-CCP was identified in 7 (8.9%) of 81 JIA patients, agreeing with the reported rates in most published studies, and the positivity (9.9%) was slightly improved by when anti-CCP3.1 IgG and IgA were used simultaneously to detect CCP.…”