Antibodies to Unconjugated Synthetic and Natural Secretins

Kd, Buchanan; Jd, Teale; Harper, Given

doi:10.1055/s-0028-1097109

Cited by 17 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Secretin was labelled with 1125 (Radiochemical Centre, Amersham) by the method of Holohan [9] and antibodies were raised to both synthetic secretin and pork natural secretin [10] (kindly donated by Dr. V. Mutt). Two antibodies were used in the studies BB33 raised against synthetic secretin (final titre 1:20,000 to 36,000); and BB101 raised against natural secretin (final titre 1 : 36,000).…”

Section: Methodsmentioning

confidence: 99%

Secretin and insulin: Response to intraduodenal acid

et al. 1976

View full text Add to dashboard Cite

Summary.Immunoreactive secretin (IRS) and immunoreactive insulin (IRI) levels were measured in humans and dogs following the intraduodenal instillation of hydrochloric acid. IRS levels rose after acid in both instances, but a concomitant rise in peripheral IRI levels was not noted. Premedication of the humans with Scopolamine prevented a rise of IRS in the human subjects. It is concluded that the endogenous release of IRS alone does not result in increased IRI levels in peripheral blood and that IRS release may be under vagal control.

show abstract

Section: Methodsmentioning

confidence: 99%

Secretin and insulin: Response to intraduodenal acid

et al. 1976

View full text Add to dashboard Cite

show abstract

“…Secretin was labelled with 125I (Radiochemical Centre, Amersham) by the method of Holohan et al (1973) and antibodies were raised to pork natural secretin (Buchanan, Teale, and Harper, 1972) (donated by Dr. V. Mutt). The antibody BB101 was used at a final titre of 1:36 000.…”

mentioning

confidence: 99%

Neonatal secretion of secretin.

Rogers¹,

Davidson²,

Lawrence³

et al. 1975

Archives of Disease in Childhood

View full text Add to dashboard Cite

“…Brown, Vancouver University, Canada), cholecystokinin-pancreozymin (both donated by Professor V. Mutt, Karolinska Institute, Stockholm) or human synthetic gastrin. Extracts of human jejunum cross-reacted in the assay in an identical manner to the standards and using an ion exchange purified 1 ~25 secretin [16] a sensitivitiy of 6 ng/1 was achieved, with 95% certainty over a range 0-300 ng/1.…”

Section: Methodsmentioning

confidence: 99%

“…Secretin was labelled with 1125 (Radiochemical Centre, Amersham, Bucks, UK) by the method of Holohan et al [15] and antibodies were raised to porcine natural secretin [16]. This antibody (BBI01) was used in a final titre of 1:36,000 and reacts with the Cterminal region of secretin.…”

Section: Methodsmentioning

confidence: 99%

The gastro-entero-pancreatic hormone response to fasting in obesity

et al. 1981

View full text Add to dashboard Cite

Summary.A comparison of the metabolic and gastroentero-pancreatic hormonal responses of ten obese and eight lean subjects to 12 h and 36 h fasts has been made. Each subject was given a 50 g oral glucose tolerance test at the end of both 12 h and 36 h starvation. After the 12 h fast blood glucose and 3-hydroxybutyrate were similar in each group but blood glycerol was 30% higher in the obese subjects. Plasma insulin and vaso-active intestinal polypeptide were also higher in the obese subjects after 12 h starvation. After 36 h starvation in the lean subjects blood glucose was unchanged but on refeeding with 50 g oral glucose, glucose tolerance was impaired. In the same group blood glycerol and 3-hydroxybutyrate rose after 36 h starvation. Plasma glucagon, secretin and vaso-active intestinal polypeptide rose after 36 h starvation in the lean subjects but plasma insulin was unchanged. Refeeding with oral glucose suppressed the increased plasma glucagon, secretin and vaso-active intestinal polypeptide. After the 36 h fast in the obese subjects, blood glucose was unchanged, blood glycerol fell, but blood 3-hydroxybutyrate rose although to a reduced level in comparison with the lean subjects. In the obese group there was no change in plasma glucagon, secretin or vaso-active intestinal polypeptide after 36 h starvation, although plasma insulin fell. The results show different metabolic and gastro-entero-pancreatic hormonal responses to fasting in lean and obese human subjects and suggest an important metabolic role of glucagon, secretin and vaso-active intestinal polypeptide during starvation.Key words: Insulin, glucagon, secretin, vaso-active intestinal polypeptide, glucose, glycerol and 3-hydroxybutyrate. * Now Endocrinology Fellow, University of Texas at DallasObesity and its consequences are a major cause of disability in the Western World, and in many obese patients dietary therapy may be ineffective. Of major interest and possible therapeutic relevance are the metabolic and hormonal abnormalities previously described during fasting in obesity, which suggest impaired lipolysis [1][2][3][4][5][6][7]28].Within the gastro-entero-pancreatic hormones there is a group with structural homology (glucagon, secretin and vaso-active intestinal polypeptide -VIP) all of which exhibit lipolytic actions in vitro [8-11, 22, 23]. To examine whether these hormones have metabolic roles in vivo and to determine whether there is an abnormal response of this group to fasting in obesity, the plasma glucagon, secretin, VIP and insulin responses together with the blood glucose, glycerol and 3-hydroxybutyrate responses to 36 h starvation have been measured in lean and obese subjects. Subjects and MethodsTen obese subjects (two males and eight females, aged 21-51 years) all with a body mass index of 29 or greater [30] and eight lean controls (three males and five females, aged 17-41 years) were fasted overnight (12 h). A 50 g oral glucose tolerance test was then administered and peripheral venous blood samples were taken at 30 rain intervals...

show abstract

Antibodies to Unconjugated Synthetic and Natural Secretins

Abstract: Antibodies to secretin have been reported using crude secretin conjugated to albumin (Young, Lazarus, Chisholm and At• kinson 1967). The present study reports the production of antibodies against microgram quantities of synthetic secretin and natural secretin in the unconjugated form.

Cited by 17 publications

References 0 publications

Secretin and insulin: Response to intraduodenal acid

Secretin and insulin: Response to intraduodenal acid

Neonatal secretion of secretin.

The gastro-entero-pancreatic hormone response to fasting in obesity

Contact Info

Product

Resources

About