Antibody-coated electrodes for detecting somatic exocytosis of somatostatin-like material in Helix neurones

Darbon, Pascal; Monnier, Zohreh; Bride, M; Crest, Marcel; Gola, Maurice; Marianneau, G.; Math, François

doi:10.1016/0165-0270(96)00053-2

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…Uptake of LY into membrane-bound vesicles occurs at the somata of PC nonburster neurons, perhaps as a concomitant of somatic release and recycling of synaptic vesicles. Somatic exocytosis of transmitter vesicles has been shown for an identified dopamine neuron in the aquatic snail Planorbis (Anderson et al 1999;Chen et al 1995) and somatostatin-containing neurons in the terrestrial snail Helix (Darbon et al 1996). The somatic vesicles of somatostatin-containing Helix neurons incorporated the fluorescent dye FM1-43 after producing trains of action potentials, confirming that spike-triggered exocytosis and vesicle recycling occurred at the soma.…”

Section: Discussionmentioning

confidence: 73%

Model for Olfactory Discrimination and Learning inLimaxProcerebrum Incorporating Oscillatory Dynamics and Wave Propagation

Ermentrout

Wang²,

Flores³

et al. 2001

Journal of Neurophysiology

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We extend our model of the procerebral (PC) lobe of Limax, which is comprised of a layer of coupled oscillators and a layer of memory neurons, each layer 4 rows by 20 columns, corresponding to the cell body layer (burster cells) and neuropil layer (nonburster cells) of the PC lobe. A gradient of connections in the layer of model burster cells induces periodic wave propagation, as measured in the PC lobe. We study odor representations in the biological PC lobe using the technique of Kimura and coworkers. Lucifer yellow injection into intact Limax after appetitive or aversive odor learning results in a band or patch of labeled cells in the PC lobe with the band long axis normal to the axis of wave propagation. Learning two odors yields two parallel bands of labeled PC cells. We introduce olfactory input to our model PC lobe such that each odor maximally activates a unique row of four cells which produces a short-term memory trace of odor stimulation. A winner-take-all synaptic competition enabled by collapse of the phase gradient during odor presentation produces a single short-term memory band for each odor. The short-term memory is converted to long-term memory if odor stimulation is followed by activation of an input pathway for the unconditioned stimulus (US) which presumably results in release of one or more neuromodulatory amines or peptides in the PC lobe.

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Section: Discussionmentioning

confidence: 73%

Model for Olfactory Discrimination and Learning inLimaxProcerebrum Incorporating Oscillatory Dynamics and Wave Propagation

Ermentrout

Wang²,

Flores³

et al. 2001

Journal of Neurophysiology

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“…This detection method has already been successfully used for detection of somatostatin-like somatic exocytosis on snail visceral ganglion neurons either due to the spontaneous activity of cultured neurons or after in situ electrical stimulation of isolated neurons .…”

mentioning

confidence: 99%

Immunoelectrodes in Protein Detection: Comparison between Glassy Carbon and a Semimetallic Ni/P Thin Film as Binding Support. Biological Applications

et al. 1998

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Though immunoelectrodes can allow direct detection of very low protein amounts (about 0.1 pmol) in vitro and in vivo, they are not yet widely used because they need quality improvement. Based on a few works devoted to the basic electrochemical phenomenon occurring when antibodies are linked onto a solid support and during antigen/antibody complex formation, we have coated two different supports with antibodies: the classical glassy carbon fiber or an epoxy plate covered with an amorphous semimetallic (nickel/phosphorus) thin film obtained by means of an electrochemical deposit. The antibody/antigen complex formation induces direct and/or indirect ionic movements and a current flow through the conductive support toward a very low-noise and high-sensitivity preamplifier stage in an I/V configuration. The proposed electrochemical treatment (hydrophilization), applied to both carbon and Ni/P electrodes, improves antibody binding and reliability of the response to antigens. The Ni/P probes present several advantages when compared to carbon fiber: better conductivity, possibility of surface quality control, and semimetallic nature, making them unbreakable. Several applications were proposed: somatostatin-14 detection with both carbon fiber and Ni/P plate electrodes, and histamine detection in simple and complex fluid media. Dose-response curves and analysis of the results lead us to conclude that the obtained currents are directly related to the quantity of antigen.

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Antibody-coated electrodes for detecting somatic exocytosis of somatostatin-like material in Helix neurones

Cited by 2 publications

References 15 publications

Model for Olfactory Discrimination and Learning inLimaxProcerebrum Incorporating Oscillatory Dynamics and Wave Propagation

Model for Olfactory Discrimination and Learning inLimaxProcerebrum Incorporating Oscillatory Dynamics and Wave Propagation

Immunoelectrodes in Protein Detection: Comparison between Glassy Carbon and a Semimetallic Ni/P Thin Film as Binding Support. Biological Applications

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