Antibody-functionalized polydiacetylene coatings on nanoporous membranes for microorganism detection

Abstract

The preparation and characterization of coatings made from polydiacetylene colloids on nano- and microporous membranes and their potential for the detection of microorganisms are presented.

The influence of amino acid sequence on structure and morphology of polydiacetylene containing peptide fibres

“…Furthermore, insight into important structural and functional features of proteins can be obtained using these small molecules, and their design can easily be varied in order to explore their structure-function relationship. 16,[24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40] Interestingly, the topochemical polymerisation diacetylenes can only take place if these moieties are closely packed, as may be the case in for example crystals, but also in self-assembled systems. A surprisingly large variety of PAs assemble into a bre-like shape, [6][7][8][9][10][11][12][13] and most are designed by using hydrophobicity as the main driving force, whereas the hydrophilic head group of the amphiphile contains structural or functional information.…”

Fabrication, structural characterization and sensing properties of polydiacetylene nanofibers templated from anodized aluminum oxide

“…Upon external perturbation, such as temperature change [2][3][4], pH [5,6] or mechanical stress [7,8], ligand-receptor interaction [9,10], the conjugated poly(ene-yne) backbone can undergo a drastic reversible or irreversible change in the relative intensity of these two phases, which is even visible to naked eyes. By making use of this sharp blue to red color change, PDAs have been extensively explored as chemical or biological sensors to monitor species ranging from small organic molecules, bio-macromolecules and even to virus and bacteria [11][12][13][14][15][16][17]. Furthermore, the red-phase PDA also has a strong fluorescence while the blue one does not, which can be used potentially in other fields including cell imaging and information storage [18][19][20][21][22][23][24].…”

Effect of phospholipid insertion on arrayed polydiacetylene biosensors

“…The vesicles show a bichromic property from blue to red upon external perturbations, such as heat, pH, and mechanical stresses, providing a cheap and convenient sensor [1][2][3]. Using the property, PDA-based sensors have been developed to detect biological samples, including influenza virus, cholera toxin, Escherichia coli, oligonucleotides, antibodies, antigens, and lipopolysaccharides [4][5][6][7][8][9][10][11][12][13]. The colorimetric change causes vesicles to turn fluorescent [3,14,15].…”