Antibody immobilization using heterobifunctional crosslinkers

Shriver‐Lake, Lisa C.; Donner, Brian L.; Edelstein, Rebecca L.; Breslin, Kristen; Bhatia, Suresh K.; Ligler, Frances S.

doi:10.1016/s0956-5663(97)00070-5

Cited by 129 publications

(73 citation statements)

References 9 publications

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“…The hydrophobic silicon surface modified with dichlordimethylsilane is often used to adsorb proteins [4], but it involves some limitation. The adsorbed proteins suffer partial denaturation and tend to leach or wash off the surface [5,6]. The silicon surface was modified with 3-aminopropyltriethoxysilane (APTES), then the amine group of APTES was reacted with glutaraldehyde to yield aldehyde group that could form an imine linkage with the primary amine group on proteins.…”

Section: Introductionmentioning

confidence: 99%

Silicon surface modification with a mixed silanes layer to immobilize proteins for biosensor with imaging ellipsometry

Wang

Jin

2004

Colloids and Surfaces B: Biointerfaces

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One kind of surface modification method on silicon wafer was presented in this paper. A mixed silanes layer was used to modify silicon surface and rendered the surface medium hydrophobic. The mixed silanes layer contained two kinds of compounds, aminopropyltriethoxysilane (APTES) and methyltriethoxysilane (MTES). A few of APTES molecules in the layer was used to immobilize covalently human immunoglobulin G (IgG) on the silicon surface. The human IgG molecules immobilized covalently on the modified surface could retain their structures well and bind more antibody molecules than that on silicon surface modified with only APTES. This kind of surface modification method effectively improved the sensitivity of the biosensor with imaging ellipsometry.

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Section: Introductionmentioning

confidence: 99%

Silicon surface modification with a mixed silanes layer to immobilize proteins for biosensor with imaging ellipsometry

Wang

Jin

2004

Colloids and Surfaces B: Biointerfaces

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“…Standard methods use protein surface groups, such as lysine, cysteins and (reduced) cystines [88], and (oxidized) carbohydrates [89,90] for either a directed immobilization at the surface or to attach a linker group including heterobifunctional linker [91], acrylic acid derivatives [67], biotin [74], and phenylboronic acid [92]. These reactive groups bind to surfaces activated with electrophiles such as NHS ester and epoxy groups (lysine, cysteine and reduced cystine), aldehydes (lysine), maleimide (cysteine and reduced cystine), amino groups (oxidized carbohydrates), and salicylhydroxamic acid (phenylboronic acid).…”

Section: Immobilization Strategies For Different Probesmentioning

confidence: 99%

Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

Sobek¹,

Bartscherer²,

Jacob³

et al. 2006

CCHTS

110

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Over the last years microarray technology has become one of the principal platform technologies for the highthroughput analysis of biological systems. Starting with the construction of first DNA microarrays in the 1990s, microarray technology has flourished in the last years and many different new formats have been developed. Peptide and protein microarrays are now applied for the elucidation of interaction partners, modification sites and enzyme substrates. Antibody microarrays are envisaged to be of high importance for the high-throughput determination of protein abundances in translational profiling approaches. First cell microarrays have been constructed to transform microarray technology from an in vitro technology to an in vivo functional analysis tool. All of these approaches share a common prerequisite: the solid support on which they are generated. The demands on this solid support are thereby as manifold as the applications themselves. This review is aimed to display the recent developments in surface chemistry and derivatization, and to summarize the latest developments in the different application areas of microarray technology.

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“…In order to covalently bind the PRMs, metal or silica surfaces are photolithographically etched and proteins are attached via a chemical linker such as aminosilane capable of forming covalent bonds with both array surface and PRM [165]. Alternatively, substances such as glutaraldehyde and N-succinimidyl-4-maleimidobutyrate may be used as cross-linkers [165][166][167].…”

Section: Arraying Of Proteinaceous Prmsmentioning

confidence: 99%

Arrays for protein expression profiling: Towards a viable alternative to two-dimensional gel electrophoresis?

2001

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Two-dimensional gel electrophoresis (2-DE) is used as a platform method for the measurement of protein expression patterns within cells, tissues or organisms. This approach can support expression profiling of several thousand proteins in multiple samples and as such it is currently unrivaled as a tool for the analysis of protein expression, which is a key component of the rapidly expanding field of proteomics. However, 2-DE has a number of significant limitations and as a consequence, alternative approaches for the measurement of expression of proteins within complex samples are actively being explored. Here we review some existing and emerging methods for protein expression analysis. In particular, we review a range of technologies that might be integrated to support the development of 'arrays' or 'chips' for rapid, high-throughput analysis of protein expression in a manner analogous to the current use of DNA arrays for mRNA expression analysis. We conclude that such separation-independent platforms may ultimately supersede two-dimensional (2-D) gel-based analyses for global protein expression analysis but that before this the technologies might provide important new platforms for diagnostic and prognostic monitoring of diseases.

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Antibody immobilization using heterobifunctional crosslinkers

Cited by 129 publications

References 9 publications

Silicon surface modification with a mixed silanes layer to immobilize proteins for biosensor with imaging ellipsometry

Silicon surface modification with a mixed silanes layer to immobilize proteins for biosensor with imaging ellipsometry

Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

Arrays for protein expression profiling: Towards a viable alternative to two-dimensional gel electrophoresis?

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