Antibody isotype responses, infection and re-infection for Schistosoma japonicum in a marshland area of China

Li, Yue Sheng; Ross, Allen G.; Sleigh, Adrian; Yi, Li; Waine, G.J.; Williams, Gail M.; Tanner, Marcel; McManus, Donald P.

doi:10.1016/s0001-706x(99)00019-4

Cited by 28 publications

(32 citation statements)

References 31 publications

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“…S. japonicum SWA were prepared by harvesting the soluble fraction obtained from sonicated S. japonicum adult worms as previously described [42]. S. japonicum eggs were extracted from the livers of infected rabbits and enriched.…”

Section: Methodsmentioning

confidence: 99%

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Dynamics of Th17 Cells and Their Role in Schistosoma japonicum Infection in C57BL/6 Mice

Wen

Cheng

et al. 2011

PLoS Negl Trop Dis

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BackgroundThe current knowledge of immunological responses to schistosomiasis, a major tropical helminthic disease, is insufficient, and a better understanding of these responses would support vaccine development or therapies to control granuloma-associated immunopathology. CD4+ T cells play critical roles in both host immune responses against parasitic infection and immunopathology in schistosomiasis. The induction of T helper (Th)1, Th2 and T regulatory (Treg) cells and their roles in schistosome infections are well-illustrated. However, little in vivo data are available on the dynamics of Th17 cells, another important CD4+ T cell subset, after Schistosoma japonicum infection or whether these cells and their defining IL-17 cytokine mediate host protective responses early in infection.MethodologyLevels of Th17 and the other three CD4+ T cell subpopulations and the cytokines related to induction or repression of Th17 cell generation in different stages of S. japonicum infection were observed. Contrary to reported in vitro studies, our results showed that the Th17 cells were induced along with the Th1, Th2, Treg cells and the IFN-γ and IL-4 cytokines in S. japonicum infected mice. The results also suggested that S. japonicum egg antigens but not adult worm antigens preferentially induced Th17 cell generation. Furthermore, decreasing IL-17 with a neutralizing anti-IL-17 monoclonal antibody (mAb) increased schistosome-specific antibody levels and partial protection against S. japonicum infection in mice.ConclusionsOur study is the first to report the dynamics of Th17 cells during S. japonicum infection and indicate that Th17 cell differentiation results from the integrated impact of inducing and suppressive factors promoted by the parasite. Importantly, our findings suggest that lower IL-17 levels may result in favorable host protective responses. This study significantly contributes to the understanding of immunity to schistosomiasis and may aid in developing interventions to protect hosts from infection or restrain immunopathology.

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Section: Methodsmentioning

confidence: 99%

“…The SWA and SEA specific IgG, IgG1 and IgG2a antibodies in mouse serum samples were detected by standard ELISA using the SWA and SEA as the coated antigen [42], [43]. HRP-conjugated rat anti-mouse IgG (Calbiochem, Darmstadt, Germany), IgG1 and IgG2a monoclonal antibodies (mAbs) (BD Pharmingen) were used.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of Th17 Cells and Their Role in Schistosoma japonicum Infection in C57BL/6 Mice

Wen

Cheng

et al. 2011

PLoS Negl Trop Dis

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“…26 Several studies have also shown a decrease in IgG4 levels against adult worm antigens months after treatment, despite the fact that other studies did not. 24,27,48 Overall, the data seem to support the use of IgG4 levels against Schistosoma antigens as representative of active, untreated infection, regardless of subject age, as long as there has been an~6-to 12-month window since treatment.…”

Section: Discussionmentioning

confidence: 69%

“…In summary, although the level of antiparasite IgG4 tends to peak in younger age groups, it is also found to be elevated in active Schistosoma infections among adults. 40,48 In our evaluation, although our sampling strategy and small sample size of the triple test group did not allow for a breakdown of the test characteristic analysis by age, it does not seem that IgG4 positivity differed greatly depending on age in those participants positive for S. haematobium infection by egg counting (Table 4). Furthermore, age distribution was fairly uniform across both the ELISA subset and the group as a whole.…”

Section: Discussionmentioning

confidence: 91%

Development of a Specimen-Sparing Multichannel Bead Assay to Detect Antiparasite IgG4 for the Diagnosis of Schistosoma and Wuchereria Infections on the Coast of Kenya

DuVall¹,

Fairley²,

Sutherland³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. To better delineate the impact of parasitic coinfection in coastal Kenya, we developed a novel specimensparing bead assay using multiplex flow immunoassay (MFI) technology to simultaneously measure serum or plasma immunoglobulin G4 (IgG4) against Brugia malayi antigen (BMA) and Schistosoma haematobium soluble worm antigen (SWAP). Properties of the bead assay were estimated by latent class analysis using data from S. haematobium egg counts/ filarial rapid diagnostic cards (RDTs), parasite-specific enzyme-linked immunosorbent assays (ELISAs), and the multichannel IgG4 assay. For schistosomiasis, the bead assay had an estimated sensitivity of 81% and a specificity of 45%, and it was more sensitive than ELISA or urine egg counts for diagnosing infection. For filariasis, it had a sensitivity of 86% and a specificity of 39%, and it was more sensitive than ELISA or RDT. Measuring antibody by MFI is feasible and may provide more accurate epidemiological information than current parasitological tests, especially in the setting of low-intensity infections.

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“…Numbers in parentheses indicate the number of sera in each group. gens, such as CEF6 (11), cathodic circulating antigen (24), and the recombinant antigens rTEG of Schistosoma japonicum (17) and r22 kDa of S. mansoni, have demonstrated acceptable sensitivities, although they are less than those of tests using the crude antigens from adult worms and eggs. Heterologous antigens have been used in the immunodiagnosis of different parasite infections.…”

Section: Figmentioning

confidence: 99%

Utility ofSchistosoma bovisAdult Worm Antigens for Diagnosis of Human Schistosomiasis by Enzyme-Linked Immunosorbent Assay and Electroimmunotransfer Blot Techniques

Pardo

Carranza

Turrientes

et al. 2004

Clin Vaccine Immunol

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Immunodiagnostic methods based on the detection of antibodies continue to be the most effective and practical methods for the diagnosis of imported schistosomiasis. Schistosoma bovis is a species whose final natural hosts are bovines, ovines, caprines, and small wild ruminants. Different studies have demonstrated the analogies existing between S. bovis and other Schistosoma species which affect humans. The objective of this work was to evaluate the utility of S. bovis adult worm antigens (AWA) for the diagnosis of imported human schistosomiasis by enzyme-linked immunosorbent assay (ELISA) and electroimmunotransfer blotting (EITB) techniques. By detecting eggs, the ELISA for S. bovis AWA was able to definitively detect imported cases with a sensitivity of 94%. The specificity of the ELISA for S. bovis AWA was 97%. There were no differences between the results of the S. bovis AWA ELISA for patients infected with Schistosoma mansoni and those infected with Schistosoma haematobium. The EITB technique showed bands of 85, 37, and 20 kDa, which are characteristic of infections with Schistosoma spp. Specific bands to indicate infection by different species of Schistosoma have not been detected. The combined use of the ELISA for S. bovis AWA and EITB increased the global sensitivity of the study to 97%. Our findings suggest that the ELISA for S. bovis AWA is a useful test for the immunodiagnosis of imported schistosomiasis and that EITB for detecting S. bovis AWA permits the confirmation of diagnosis when the ELISA for S. bovis AWA is positive.

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Antibody isotype responses, infection and re-infection for Schistosoma japonicum in a marshland area of China

Cited by 28 publications

References 31 publications

Dynamics of Th17 Cells and Their Role in Schistosoma japonicum Infection in C57BL/6 Mice

Dynamics of Th17 Cells and Their Role in Schistosoma japonicum Infection in C57BL/6 Mice

Development of a Specimen-Sparing Multichannel Bead Assay to Detect Antiparasite IgG4 for the Diagnosis of Schistosoma and Wuchereria Infections on the Coast of Kenya

Utility ofSchistosoma bovisAdult Worm Antigens for Diagnosis of Human Schistosomiasis by Enzyme-Linked Immunosorbent Assay and Electroimmunotransfer Blot Techniques

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