Utility of<i>Schistosoma bovis</i>Adult Worm Antigens for Diagnosis of Human Schistosomiasis by Enzyme-Linked Immunosorbent Assay and Electroimmunotransfer Blot Techniques

Pardo, Jesús Espeja; Carranza, Cristina; Turrientes, María-Carmen; Arellano, Jose Luis Pérez; Velez, Rita; Ramajo, V.; Muro, Antonio

doi:10.1128/cdli.11.6.1165-1170.2004

Cited by 36 publications

(17 citation statements)

References 30 publications

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“…Most likely, species-specific heterogeneities of the utilized antigens were responsible for the observed differences. Although it has been shown that S. mansoni proteins used for serological testing have sufficient similarity to allow serodiagnosis of S. haematobium infections (32), some species-specific differences remain, favoring test systems using homologous antigens (2,19,22,30,36,39,42). Nevertheless, species-specific performance differences were not determined only by the utilized antigens.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Eight Serological Tests for Diagnosis of Imported Schistosomiasis

Kinkel

Dittrich

Baümer

et al. 2012

Clin Vaccine Immunol

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ABSTRACTThe diagnosis of schistosomiasis in individuals from countries where the disease is not endemic is challenging, and few data are available on the accuracy of serological diagnosis in those patients. We evaluated the performance of eight serological assays, including four commercial kits, in the diagnosis of imported schistosomiasis in individuals from areas where the disease is not endemic, including six enzyme-linked immunosorbent assays using three different antigens, an indirect hemagglutination assay, and an indirect immunofluorescent-antibody test. To analyze the assays, we used a total of 141 serum samples, with 121 derived from patients with various parasitic infections (among which were 37 cases of schistosomiasis) and 20 taken from healthy volunteers. The sensitivity values for detection of schistosomiasis cases ranged from 41% to 78% and were higher forSchistosoma mansonithan forS. haematobiuminfections. Specificity values ranged from 76% to 100%; false-positive results were most frequent for samples from patients with cestode infections. By combining two or more tests, sensitivity improved markedly and specificity decreased only moderately. Serological tests are useful instruments for diagnosing imported schistosomiasis in countries where the disease is not endemic, but due to limitations in test sensitivities, we recommend the use of two or more assays in parallel.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Eight Serological Tests for Diagnosis of Imported Schistosomiasis

Kinkel

Dittrich

Baümer

et al. 2012

Clin Vaccine Immunol

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“…The Specific Worm Antigen Product (SWAP) was obtained as previously described (Pardo et al 2004). ELISAs were performed by coating polystyrene plates (Costar) with 0.1 ml of 10 mg/ml SWAP in Phosphate Buffer Saline (PBS) overnight at 4°C.…”

Section: Quantification Of Specific Antibodies Against S Mansonimentioning

confidence: 99%

Identifying phenotypes involved in susceptibility to Schistosoma mansoni infection in F1B6CBA mice

Villar

Vicente

Blanco-Gómez

et al. 2014

Acta Parasitologica

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Schistosomiasis is a disease with a strong genetic component influenced by socioeconomic and ecological factors. Epidemiological studies have identified several genetic regions involved in the schistosomiasis susceptibility. However, it is not well known what physiological traits are predisposing to the disease. The study of experimental infections in inbred mouse strains with variable genetic susceptibility to the disease offers a good opportunity to tackle this question. F1B6CBA hybrid between the most divergent strains was infected in order to characterize the immunophenotypes that correlate with the susceptibility of schistosomiasis disease in mice. Complete blood counts and immunophenotype were determined at 0, 3, 6, and 9 weeks post infection. Nine weeks after cercariae exposure, animals were perfused and worm recovery was assessed. A large number of hepatic lesions, a reduction in the eosinophil and basophil count in the acute phase of infection and the decreased number of monocytes, neutrophils and B-lymphocytes are phenotypes associated with increased susceptibility to S. mansoni infection.

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“…This assay employed the qualitative enzyme immunoassay technique. 20 The crude eggsoluble antigen (50 μL) of 3 μg/mL diluted 1:200 in carbonate buffer (pH 9.6) was used to coat the microtiter well plates and then incubated overnight at 4 °C. This was followed by washing of plates with 0.05% Tween 20 in PBS-Tween 20, thrice.…”

Section: Serological Assaysmentioning

confidence: 99%

Anti-SchistosomaIgG responses inSchistosoma haematobiumsingle and concomitant infection with malaria parasites

Morenikeji

Adeleye

Omoruyi

et al. 2016

Pathogens and Global Health

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Areas prone to schistosomiasis are also at risk of malaria transmission. The interaction between the causal agents of the two diseases could modulate immune responses tailored toward protecting or aggravating morbidity dynamics and impair Schistosoma diagnostic precision. This study aimed at assessing the effect of Plasmodium spp. in concomitant infection with Schistosoma haematobium in modulation of anti-Schistosoma IgG antibodies. The school-based cross-sectional study recruited a total of 322 children screened for S. haematobium and Plasmodium spp. Levels of IgG against S. haematobium-soluble egg antigen (SEA) in single S. haematobium/ malaria parasites infection and co-infection of the two parasites in schoolchildren were determined. Data were analyzed using χ 2 , Fisher's exact test, and Tukey's multiple comparison test analyses. The prevalence of single infection by S. haematobium, Plasmodium spp., and concurrent infection due to the two pathogens was 27.7, 41.0, and 9.3%, respectively (p < 0.0001). Anti-Schistosoma IgG production during co-infection of the two pathogens (1.950 ± 0.742 AU) was significantly higher than the value recorded for single malaria parasites' infection (1.402 ± 0.670 AU) (p < 0.01) but not in S. haematobium infection (1.591 ± 0.604 AU) (p > 0.05). The anti-Schistosoma IgG production in co-infection status was however dependent on the intensity of Plasmodium spp. with individuals having high intensity of malaria parasites recording lower anti-Schistosoma IgG. This study has implication for diagnosis of schistosomiasis where anti-Schistosoma IgG is used as an indicator of infection. Efforts should be made to control the two infections simultaneously in order not to undermine the efforts targeted toward the control of one.

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Utility ofSchistosoma bovisAdult Worm Antigens for Diagnosis of Human Schistosomiasis by Enzyme-Linked Immunosorbent Assay and Electroimmunotransfer Blot Techniques

Cited by 36 publications

References 30 publications

Evaluation of Eight Serological Tests for Diagnosis of Imported Schistosomiasis

Evaluation of Eight Serological Tests for Diagnosis of Imported Schistosomiasis

Identifying phenotypes involved in susceptibility to Schistosoma mansoni infection in F1B6CBA mice

Anti-SchistosomaIgG responses inSchistosoma haematobiumsingle and concomitant infection with malaria parasites

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