Antibody isotypes profiles against Trypanosoma cruzi acidic antigens in two Amerindian populations from a Chagas' disease endemic area

Motrán, Claudia Cristina; Serra, Horacio M.; Gea, Susana; Vullo, Carlos; Vottero-Cima, E

doi:10.1016/0001-706x(94)90050-7

Cited by 8 publications

(10 citation statements)

References 25 publications

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“…This finding is discrepant to those previously reported by several other authors [7][8][9][10]13,[26][27] . Only at serum dilution 1:10 was a significant difference in the OD of IgG1 observed between the IND and CARD groups.…”

Section: Discussioncontrasting

confidence: 99%

In-house ELISA method to analyze anti-Trypanosoma cruzi IgG reactivity for differential diagnosis and evaluation of Chagas disease morbidity

Santos

Torres

Machado-de-Assis

et al. 2012

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: The goal was to develop an in-house serological method with high specificity and sensitivity for diagnosis and monitoring of Chagas disease morbidity. METHODS: With this purpose, the reactivities of anti-T. cruzi IgG and subclasses were tested in successive serum dilutions of patients from Berilo municipality, Jequitinhonha Valley, Minas Gerais, Brazil. The performance of the in-house ELISA was also evaluated in samples from other relevant infectious diseases, including HIV, hepatitis C (HCV), syphilis (SYP), visceral leishmaniasis (VL), and American tegumentary leishmaniasis (ATL), and noninfected controls (NI). Further analysis was performed to evaluate the applicability of this in-house methodology for monitoring Chagas disease morbidity into three groups of patients: indeterminate (IND), cardiac (CARD), and digestive/mixed (DIG/Mix), based on their clinical status. RESULTS: The analysis of total IgG reactivity at serum dilution 1:40 was an excellent approach to Chagas disease diagnosis (100% sensitivity and specificity). The analysis of IgG subclasses showed cross-reactivity, mainly with NI, VL, and ATL, at all selected serum dilutions. Based on the data analysis, the IND group displayed higher IgG3 levels and the DIG/Mix group presented higher levels of total IgG as compared with the IND and CARD groups. CONCLUSIONS: These findings demonstrated that methodology presents promising applicability in the analysis of anti-T. cruzi IgG reactivity for the differential diagnosis and evaluation of Chagas disease morbidity.

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Section: Discussioncontrasting

confidence: 99%

In-house ELISA method to analyze anti-Trypanosoma cruzi IgG reactivity for differential diagnosis and evaluation of Chagas disease morbidity

Santos

Torres

Machado-de-Assis

et al. 2012

Rev. Soc. Bras. Med. Trop.

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“…Investigation into specific immunoglobulins in the serum of individuals with Chagas' disease, together with the use of pure and specific antigens, may be a key combination in elucidating the factors that bring about and maintain the clinical manifestations of the disease (3,8).…”

Section: Discussionmentioning

confidence: 99%

“…The identification of target molecules using pure proteins, chemically defined and specific to the parasite has been suggested by various authors as a way of finding out more about the immunopathology of Chagas' disease (3,8). Two antigens with these characteristics were used in this study: cytoplasmatic repetitive antigen (CRA), present in evolved forms of the epimastigote and amastigote of T. cruzi, and flagellar repetitive antigen (FRA), present in the epimastigote and trypomastigote forms of the parasite (9,10).…”

Section: Introductionmentioning

confidence: 99%

Chagas' disease: IgG isotypes against cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens of Trypanosoma cruzi in chronic Chagasic patients

Verçosa

Lorena

Carvalho

et al. 2007

Clinical Laboratory Analysis

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The wide range of clinical Chagas' disease manifestations, of which heart involvement is the most significant, because of its characteristics, frequency and consequences, and lack of treatment and cure, justify research in this area. Specific immunoglobulin G (IgG) antibody subclasses have been associated with human Chagas' disease. Thus, in this study, the profile of IgG subclasses against cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive T. cruzi-specific antigens was correlated with cardiac (CARD, n=33), cardiodigestive (CD, n=7), and indeterminate (IND, n=20) forms of Chagas' disease by indirect enzyme-linked immunosorbent assay (ELISA). IgG subclasses were detected in almost all Chagas patients studied. Nevertheless, only specific IgG2 isotype FRA was found with a significant statistical difference in CARD patients when compared to IND patients. This result suggests the potential use of this isotype for prognostic purposes, for monitoring the progression of chronic Chagas' disease, and for predicting the risk of CARD damage. This is important information, as it could help physicians to evaluate and manage the treatment of their patients. However, a follow-up study is necessary to confirm our result.

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“…However, no correlation with clinical forms of chronic infection has been observed. Still, it is important to emphasize that most of these studies of the IgG subclasses have been performed by immunoassays using soluble or fixed whole T. cruzi antigenic preparations (19,20,21).…”

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confidence: 99%

Anti-Trypanosoma cruziImmunoglobulin G1 Can Be a Useful Tool for Diagnosis and Prognosis of Human Chagas' Disease

Cordeiro

Martins‐Filho

Rocha

et al. 2001

Clin Diagn Lab Immunol

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Two functionally distinct antibodies, categorized as conventional serology antibodies (CSA) and lytic antibodies (LA) have been described in Chagas' disease, based on their ability to bind to fixed epimastigotes (EPI) or live trypomastigotes (TRYPO), respectively. In this study, the profile of immunoglobulin G (IgG) subclasses of CSA and LA were analyzed by flow cytometry using serum samples from chronic chagasic patients with the indeterminate (

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Antibody isotypes profiles against Trypanosoma cruzi acidic antigens in two Amerindian populations from a Chagas' disease endemic area

Cited by 8 publications

References 25 publications

In-house ELISA method to analyze anti-Trypanosoma cruzi IgG reactivity for differential diagnosis and evaluation of Chagas disease morbidity

In-house ELISA method to analyze anti-Trypanosoma cruzi IgG reactivity for differential diagnosis and evaluation of Chagas disease morbidity

Chagas' disease: IgG isotypes against cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens of Trypanosoma cruzi in chronic Chagasic patients

Anti-Trypanosoma cruziImmunoglobulin G1 Can Be a Useful Tool for Diagnosis and Prognosis of Human Chagas' Disease

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