Antibody responses induced by recombinant ALV-A gp85 protein vaccine combining with CpG-ODN adjuvant in breeder hens and the protection for their offspring against early infection

Zhang, Dandan; Li, Hongmei; Zhang, Zhongsheng; Sun, Shuhong; Cheng, Ziqiang; Liu, Jianzhu; Zhao, Peng; QingYa, Ren; Guo, Huijun

doi:10.1016/j.antiviral.2015.01.007

Cited by 13 publications

(10 citation statements)

References 21 publications

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“…In this study, DF1 cells were inoculated with the ALV-A-SDAU09C1 strain, which was isolated from imported ancestral breeding broiler chickens in 2009 [19] and was reported to replicate in DF1 cells and to be pathogenic in chickens [19,20,37]. The expression of chicken TRIM25 at both the mRNA and protein levels was significantly increased, which suggests that the antiviral responses mediated by TRIM25 were induced.…”

Section: Discussionmentioning

confidence: 93%

Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response

Zhou

Liu

Hong-mei

et al. 2020

Vet Res

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This study focuses on the immunoregulatory effects of chicken TRIM25 on the replication of subgroup A of avian leukosis virus (ALV-A) and the MDA5-mediated type I interferon response. The ALV-A-SDAU09C1 strain was inoculated into DF1 cells and 1-day-old SPF chickens, and the expression of TRIM25 was detected at different time points after inoculation. A recombinant overexpression plasmid containing the chicken TRIM25 gene (TRIM25-GFP) was constructed and transfected into DF1 cells to analyse the effects of the overexpression of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN-β. A small interfering RNA targeting chicken TRIM25 (TRIM25-siRNA) was prepared and transfected into DF1 cells to assess the effects of the knockdown of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN-β. The results showed that chicken TRIM25 was significantly upregulated at all time points both in ALV-A-infected cells and in ALV-A-infected chickens. Overexpression of chicken TRIM25 in DF1 cells dramatically decreased the antigenic titres of ALV-A in the cell supernatant and upregulated the relative expression of MDA5, MAVS and IFN-β induced by ALV-A or by poly(I:C); in contrast, knockdown of chicken TRIM25 significantly increased the antigenic titres of ALV-A and downregulated the relative expression of MDA5, MAVS and IFN-β. It can be concluded that chicken TRIM25 can inhibit the replication of ALV-A and upregulate the MDA5 receptor-mediated type I interferon response in chickens. This study can help improve the understanding of the antiviral activities of chicken TRIM25 and enrich the knowledge of antiviral responses in chickens.

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Section: Discussionmentioning

confidence: 93%

Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response

Zhou

Liu

Hong-mei

et al. 2020

Vet Res

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“…ALV-A in the liver, spleen, and heart of each bird at 21 dpi was detected by IFA using a MAb specific for the ALV-A gp85 protein 20 . Briefly, frozen sections of the tissues were prepared and fixed with acetone at 4 °C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…ALV-A antibodies in the collected serum samples from all of the birds were detected using an IDEXX ALV-A/B antibody test kit according to the published method 20 . The relative antibody titers in the serum were determined by calculating the S/P ratio [(mean of sample optical density) − (mean of negative control optical density)]/[(mean of positive control optical density) − (mean of negative control optical density)].…”

Section: Methodsmentioning

confidence: 99%

“…The combination of serum antibodies from the infected birds with the gp85 protein of ALV-A-SDAU09C1 was detected with an immune diffusion test 21 . Agar Petri dishes and sample wells were prepared, and the purified recombinant gp85 protein (rgp85) of ALV-A-SDAU09C1 20 was placed in the center sample well. Standard positive serum and dilute serum samples were alternately placed in the surrounding sample wells.…”

Section: Methodsmentioning

confidence: 99%

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Comparison of Viremia, Cloacal Virus Shedding, Antibody Responses and Pathological Lesions in Adult Chickens, Quails, and Pigeons Infected with ALV-A

Zhang

et al. 2019

Sci Rep

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Subgroup A of the avian leukosis virus (ALV-A) can cause severe pathological lesions and death in infected chickens, and its reported hosts have increased recently. To assess the susceptibility of adult chickens, quails, and pigeons to ALV-A, three sets of 250-day-old birds were intraperitoneally inoculated with ALV-A. Viremia and cloacal virus shedding were dynamically detected using an immunofluorescence assay (IFA), ALV-P27 antigen ELISA or RT-PCR; pathological lesions were assessed using tissue sections; ALV-A in tissues was detected by IFA; and ALV-A antibody responses were detected using antibody ELISA kits and an immune diffusion test. The results indicated that persistent viremia occurred in 80% (8/10) of infected chickens, and transient viremia occurred in 17% (2/12) of infected quails, but no viremia occurred in infected pigeons. Cloacal virus shedding occurred intermittently in 80% (8/10) of infected chickens and in 8% (1/12) of infected quails but did not occur in infected pigeons. Severe inflammatory pathological lesions occurred in the visceral tissues of most infected chickens, and mild lesions occurred in a few of the infected quails, but no pathological lesions occurred in the infected pigeons. The ALV-A virus was detected in the visceral tissues of most infected chickens but not in the infected quails and pigeons. Obviously different ALV-A antibody responses occurred in the infected chickens, quails and pigeons. It can be concluded that adult chickens, quails and pigeons have dramatically different susceptibilities to ALV-A. This is the first report on artificial infection by ALV-A in different birds.

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“…An ALV-A gp85 protein subunit vaccine could induce neutralizing antibody when given with a CpG-ODN adjuvant to breeder hens. This protected 80% of their offspring chickens against early infections (69). …”

Section: Immunity Against Alvmentioning

confidence: 99%

Immunity to Avian Leukosis Virus: Where Are We Now and What Should We Do?

Feng

Zhang

2016

Front. Immunol.

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Avian leukosis virus (ALV) is an avian oncogenic retrovirus causing enormous economic losses in the global poultry industry. Although ALV-related research has lasted for more than a century, there are no vaccines to protect chickens from ALV infection. The interaction between chickens and ALV remains not fully understood especially with regard to the host immunity. The current review provides an overview of our current knowledge of innate and adaptive immunity induced by ALV infection. More importantly, we have pointed out the unknown area involved in ALV-related studies, which is worthy of our serious exploring in future.

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Antibody responses induced by recombinant ALV-A gp85 protein vaccine combining with CpG-ODN adjuvant in breeder hens and the protection for their offspring against early infection

Cited by 13 publications

References 21 publications

Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response

Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response

Comparison of Viremia, Cloacal Virus Shedding, Antibody Responses and Pathological Lesions in Adult Chickens, Quails, and Pigeons Infected with ALV-A

Immunity to Avian Leukosis Virus: Where Are We Now and What Should We Do?

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