Antibody Responses to Individual Bordetella pertussis Fimbrial Antigen Fim2 or Fim3 following Immunization with the Five-Component Acellular Pertussis Vaccine or to Pertussis Disease

Antibody Responses to Bordetella pertussis Fim2 or Fim3 following Immunization with a Whole-Cell, Two-Component, or Five-Component Acellular Pertussis Vaccine and following Pertussis Disease in Children in Sweden in 1997 and 2007

“…The ELISA IgG concentration values assigned to standard serum 89/530 in the previous study (21) were determined by aligning the ELISA dose-response curve for a 1:1 mixture of Fim2 and Fim3 with the dose-response curves obtained with this serum and separate Fim2 or Fim3 prep- A unique serum collection from a well-controlled group of small children, vaccinated with three doses of either DTaP5, DTaP2, DTwP, or placebo DT and assessed previously for antiFim2/3 IgG by ELISAs (13), has allowed us to determine antiFim2 and anti-Fim3 IgG responses. Anti-Fim2 and anti-Fim3 IgG concentrations were determined in 7-month-old children in the DTaP5 and DTwP groups only.…”

“…To understand the role of antibodies to Fim2 and Fim3, we purified individual Fim2 and Fim3 from B. pertussis and used these to quantify anti-Fim2-and anti-Fim3-specific IgG following vaccination with DTaP5 in individuals with clear evidence of ongoing and recent pertussis disease (21). The ELISA IgG concentration values assigned to standard serum 89/530 in the previous study (21) were determined by aligning the ELISA dose-response curve for a 1:1 mixture of Fim2 and Fim3 with the dose-response curves obtained with this serum and separate Fim2 or Fim3 prep- A unique serum collection from a well-controlled group of small children, vaccinated with three doses of either DTaP5, DTaP2, DTwP, or placebo DT and assessed previously for antiFim2/3 IgG by ELISAs (13), has allowed us to determine antiFim2 and anti-Fim3 IgG responses.…”

“…Recently, we described the antibody response to Fim2 or Fim3 separately following immunization with DTaP5 (containing 5 g per dose Fim2/3) or pertussis disease (21). It was found that all individuals showed increases in anti-Fim2 and anti-Fim3 IgG concentrations following vaccination with DTaP5, with 3-fold greater anti-Fim2 than anti-Fim3 IgG concentrations seen in 15-month-old and 4-to 6-year-old children.…”

“…The antigens were diluted to 1.0 mg/liter in phosphate-buffered saline (PBS) (pH 7.4) and were used for to coat plates, which were stored overnight at room temperature. A National Institute for Biological Standards and Control (NIBSC) reference human antiserum (89/530), previously determined to contain 74.1 EU/ml IgG anti-Fim2 and 14.8 EU/ml IgG anti-Fim3, was used as the primary calibrator (21). A pool of fractionated human plasma (Ig42) was used as the in-house secondary calibrator.…”

Plasticity of fimbrial genotype and serotype within populations of Bordetella pertussis: analysis by paired flow cytometry and genome sequencing

“…The distribution of B. pertussis fimbrial serotypes, determined via agglutination, varies between countries and over time, and may be influenced by differing vaccination policies (reviewed by Poolman & Hallander, 2007 (Litt et al, 2009), but increased in 2011-2012(Alexander et al, 2012 to levels comparable with the pre-vaccine and whole-cell vaccine eras. It is possible that population immunity to the predominant serotype builds up until a tipping point is reached and expression of the alternative serotype is favoured.…”