Antibody to Rhinovirus in Human Sera. I. Standardization of a Neutralization Test

Rg, Douglas; Wf, Fleet; Tr, Cate; Rb, Couch

doi:10.3181/00379727-127-32724

Cited by 25 publications

(3 citation statements)

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“…However, complement ®xation [Chapple et al, 1967], hemagglutination inhibition [Reed and Hall, 1973], indirect immuno¯uorescence [Hruskova et al, 1981], macro-and microneutralization [Gwaltney, 1966;Douglas et al, 1968;Monto and Bryan, 1974], and enzyme-linked immunoassay [Barclay and Al-Nakib, 1987] have been described for measurement of rhinoviral antibodies.…”

Section: Introductionmentioning

confidence: 99%

Virological and serological analysis of rhinovirus infections during the first two years of life in a cohort of children

Blomqvist

Roivainen

Puhakka

et al. 2001

Journal of Medical Virology

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The occurrence of rhinovirus infections in a cohort of 329 children during the first 2 years of life was determined by virus detection and serological methods. Rhinovirus detection on nasopharyngeal aspirates and middle ear fluids comprised a combination of virus isolation in HeLa Ohio cells and a reverse transcription-polymerase chain reaction (RT-PCR)-hybridization assay on the inoculated cell cultures. Nasopharyngeal aspirates were collected when the child was referred to the study clinic because of respiratory symptoms. Nasopharyngeal aspirates and middle ear fluids were collected after clinical diagnosis of an acute otitis media. Complement-fixing antibodies to rhinovirus were determined from scheduled serum specimens collected at 6, 12, 18, and 24 months of age and from paired sera taken in the cases of acute otitis media. Rhinovirus infections were shown to be common in infants, 24% of the children had complement-fixing antibodies at the age of 6 months and 22% had had at least one rhinovirus episode indicated by virus detection. At the age of 2 years, 91.3% of the children had rhinovirus-specific antibodies, while 79% of the children had experienced rhinovirus infection as judged by the virus detection tests. However, the complement-fixation assay was poor as a diagnostic test. Of 458 acute otitis media episodes studied, 41% were shown to be associated with a rhinovirus by RT-PCR-hybridization, while significant fourfold rise in rhinoviral antibodies was detected only in 7% of the cases.

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Section: Introductionmentioning

confidence: 99%

Virological and serological analysis of rhinovirus infections during the first two years of life in a cohort of children

Blomqvist

Roivainen

Puhakka

et al. 2001

Journal of Medical Virology

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“…The presence of seroimmunity to a respiratory virus is generally indicative of resistance to infection by that virus (33,41). However, the protection is only relative.…”

Section: Clinicalmentioning

confidence: 99%

The Common Cold

Levandowski¹

1987

Infections of the Head and Neck

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“…Infections with RV induce typespecific antibody responses that reduce the risk of reinfections. Natural infections with RV-A and RV-B induce neutralizing antibody (nAb) responses that protect against reinfection and persist for at least 2-4 years (20)(21)(22)(23)(24). NAb responses to RV-C have not previously been analyzed because of the lack of a suitable in vitro system to assay infectivity.…”

mentioning

confidence: 99%

Enhanced Neutralizing Antibody Responses to Rhinovirus C and Age-Dependent Patterns of Infection

Choi

Devries

Bacharier

et al. 2021

Am J Respir Crit Care Med

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Rationale: Rhinovirus (RV) C can cause asymptomatic infection and respiratory illnesses ranging from the common cold to severe wheezing.Objectives: To identify how age and other individual-level factors are associated with susceptibility to RV-C illnesses.Methods: Longitudinal data from the COAST (Childhood Origins of Asthma) birth cohort study were analyzed to determine relationships between age and RV-C infections. Neutralizing antibodies specific for RV-A and RV-C (three types each) were determined using a novel PCR-based assay. Data were pooled from 14 study cohorts in the United States, Finland, and Australia, and mixed-effects logistic regression was used to identify factors related to the proportion of RV-C versus RV-A detection.Measurements and Main Results: In COAST, RV-A and RV-C infections were similarly common in infancy, whereas RV-C was detected much less often than RV-A during both respiratory illnesses and scheduled surveillance visits (P , 0.001, x 2 ) in older children. The prevalence of neutralizing antibodies to RV-A or RV-C types was low (5-27%) at the age of 2 years, but by the age of 16 years, RV-C seropositivity was more prevalent (78% vs. 18% for RV-A; P , 0.0001). In the pooled analysis, the RV-C to RV-A detection ratio during illnesses was significantly related to age (P , 0.0001), CDHR3 genotype (P , 0.05), and wheezing illnesses (P , 0.05). Furthermore, certain RV types (e.g., C2, C11, A78, and A12) were consistently more virulent and prevalent over time.Conclusions: Knowledge of prevalent RV types, antibody responses, and populations at risk based on age and genetics may guide the development of vaccines or other novel therapies against this important respiratory pathogen.

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Antibody to Rhinovirus in Human Sera. I. Standardization of a Neutralization Test

Cited by 25 publications

References 0 publications

Virological and serological analysis of rhinovirus infections during the first two years of life in a cohort of children

Virological and serological analysis of rhinovirus infections during the first two years of life in a cohort of children

The Common Cold

Enhanced Neutralizing Antibody Responses to Rhinovirus C and Age-Dependent Patterns of Infection

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