Antibody to soluble nuclear antigens in dogs (German shepherd) with a lupus like syndrome

Monier, J. C.; Schmitt, Daniel; Perraud, M.; Fleury, C.; Gioud, M; Lapras, M.

doi:10.1016/s0145-305x(78)80034-2

Cited by 14 publications

(3 citation statements)

References 12 publications

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“…However, if human criterium are strictly adhered to, most dogs with SLE' would not qualify. Dogs tend to have one or two systemic signs, often polyarthritis, while skin, hematologic, CNS, and renal manifestations are less important (Pedersen et al, 1976a;Krum et al, 1977;Monier et al, 1978;Slappendel et al, 1972).…”

Section: Systemic Lupus Erythematosus (Sle)mentioning

confidence: 99%

A review of immunologic diseases of the dog

Pedersen

1999

Veterinary Immunology and Immunopathology

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The following review is based on notes used in the teaching of clinical immunology to veterinary students. Immune diseases of the dog are placed into six different categories: (1) type I or allergic conditions; (2) type II or auto- and allo-antibody diseases; (3) type III or immune complex disorders; (4) type IV or cell-mediated immune diseases; (5) type V conditions or gammopathies; and (6) type VI or immunodeficiency disorders. Separate discussions of transplantation immunology and the use of drugs to regulate unwanted immune responses are also included.

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Section: Systemic Lupus Erythematosus (Sle)mentioning

confidence: 99%

A review of immunologic diseases of the dog

Pedersen

1999

Veterinary Immunology and Immunopathology

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“…Antibodies to Sm are found in certain SLE patients (1), dogs with a lupus-like syndrome (8), and the MRL/Mp-lpr/lpr (MRL/1) and MRL/Mp-+/+ mouse strains (9). In this paper, we report the production of monoclonal antibodies, derived from an MRL/l mouse, specific for Sm, DNA, and rRNA.…”

mentioning

confidence: 99%

Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.

Lerner¹,

Lerner²,

Janeway³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

660

427

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Mice of the strain MRL/Mp-lpr/lpr develop a lupus erythematosus-like syndrome that includes the production of autoantibodies specific for nucleic acid-containing cellular components. We have fused spleen cells from such a mouse with the myeloma SP 2/0 and examined the antibodies produced by the resultant cloned hybrid cell lines by using immunoprecipitation and immunofluorescence techniques. Three types of monoclonal antibodies, specific for Sm, DNA, or rRNA, all antigens to which patients who have lupus make antibodies, have been identified. Patient anti-Sm antibody had previously been reported to precipitate five small nuclear ribonucleoproteins that contain U-1, U-2, U-4, U-5, and U-6 RNAs. The monoclonal anti-Sm antibody gives the same immunoprecipitation pattern, providing direct evidence that the Sm antigen resides on all these RNA-protein complexes. Monoclonal anti-Sm antibody will be valuable in deciphering the biological function of these ubiquitous small nuclear RNPs. A simple competition radioimmunoassay using the monoclonal anti-Sm antibody to titer patient sera is also presented. Uses of monoclonal antibodies for the study of autoimmune disease are discussed.The sera of patients and mice that have autoimmune disorders contain antibodies whose presence often results in tissue injury. The mechanisms leading to the development of autoantibodies are not well understood. However, the appearance of specific antibodies in patient sera has been reported to be associated with particular clinical signs of disease (1). Studies with monoclonal antibodies having the same specificity as such autoantibodies could result in a clearer understanding ofboth the mechanisms leading to the formation of autoantibodies and the role of such antibodies in producing disease. Mouse monoclonal antibodies to DNA and ribosomal RNA have been described (2, 3).Autoantibodies also provide potent tools to probe the structure and function ofthe cellular constituents against which they are directed. We have previously used antibodies from the sera of patients who have systemic lupus erythematosus (SLE) to examine the antigens designated Sm, RNP, La, and Ro (4-6). Each of these antigens resides on discrete particles composed of RNA and protein and called either small nuclear ribonucleoproteins (snRNPs) or small cytoplasmic ribonucleoproteins.Anti-Sm has been found to precipitate five different snRNPs, containing the small RNAs U-1, U-2, U-4, U-5, and U-6, from mammalian cell extracts. One snRNP that bears the RNP as well as the Sm determinant appears to be involved in the nuclear splicing of mRNA precursors (7), while cellular roles for the Laand remaining Sm-bearing snRNPs and for the Ro-containing small cytoplasmic RNPs are unknown. The availability of monoclonal antibodies directed against snRNPs would eliminate many uncertainties in experimental interpretation raised by the use of patient sera.Antibodies to Sm are found in certain SLE patients (1), dogs with a lupus-like syndrome (8), and the MRL/Mp-lpr/lpr (MRL/1) and MRL/M...

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“…Several autoantigens have been previously identified in dogs by different approaches 9 , 21 , 22 , 38 , 46 – 50 . Here, we report the use of human protein arrays for the screening of canine autoantigens.…”

Section: Discussionmentioning

confidence: 99%

ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease

Bremer

Landegren

Sjöberg

et al. 2018

Sci Rep

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Dogs can spontaneously develop complex systemic autoimmune disorders, with similarities to human autoimmune disease. Autoantibodies directed at self-antigens are a key feature of these autoimmune diseases. Here we report the identification of interleukin enhancer-binding factors 2 and 3 (ILF2 and ILF3) as autoantigens in canine immune-mediated rheumatic disease. The ILF2 autoantibodies were discovered in a small, selected canine cohort through the use of human protein arrays; a method not previously described in dogs. Subsequently, ILF3 autoantibodies were also identified in the same cohort. The results were validated with an independent method in a larger cohort of dogs. ILF2 and ILF3 autoantibodies were found exclusively, and at a high frequency, in dogs that showed a speckled pattern of antinuclear antibodies on immunofluorescence. ILF2 and ILF3 autoantibodies were also found at low frequency in human patients with SLE and Sjögren’s syndrome. These autoantibodies have the potential to be used as diagnostic biomarkers for canine, and possibly also human, autoimmune disease.

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Antibody to soluble nuclear antigens in dogs (German shepherd) with a lupus like syndrome

Cited by 14 publications

References 12 publications

A review of immunologic diseases of the dog

A review of immunologic diseases of the dog

Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.

ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease

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