Anticancer Activities of Oldenlandia diffusa

Gupta, Salin; Zhang, David; Yi, Jizu; Shao, Jun

doi:10.1300/j157v04n01_03

Cited by 36 publications

(42 citation statements)

References 7 publications

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“…VEGF binds to VEGF receptor, which leads to the activation of phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway. PI3K/AKT signaling regulates angiogenesis through affecting the expression of VEGF (9)(10)(11)(12)14). In addition, forced expression of PI3K alone is sufficient to increase angiogenesis via increased VEGF expression.…”

Section: Discussionmentioning

confidence: 99%

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Bufalin enhances anti-angiogenic effect of sorafenib via AKT/VEGF signaling

Wang

Zhang

Ning

et al. 2016

International Journal of Oncology

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Abstract. Sorafenib mainly exerts its anti-hepatoma effect by inhibiting tumor angiogenesis. However, its curative effect is limited. Thus, application of drugs which could augment its anti-angiogenic effect is necessary. Bufalin has been reported to possess anticancer properties. In the present study, we investigated the synergistic anti-angiogenic effect of sorafenib combined with bufalin. The enhanced anti-angiogenic effect of the combination treatment was firstly assessed in nude mice bearing human HCC intradermal tumors. In addition, we found that proliferation was significantly inhibited and the morphology was obviously changed in the combinationtreated human umbilical vein endothelial cells (HUVEC) at 48 h of treatment. In addition, the combination treatment was found to suppress vessel formation potently as proved in the tube formation, chick chorioallantoic membrane and rat aortic rings. Mechanistically, HUVEC incubated with the combination treatment showed increased apoptosis, decreased migration, which might account for its capacity against angiogenesis. Vascular endothelial cells have been reported to secrete cytokines to affect angiogenesis. Therefore, suspensions from HUVECs with different treatments were collected as conditioned medium (CM). The combinationtreated CM significantly inhibited the migration of HUVEC and blood vessel formation in vitro. Importantly, multiple cytokines associated with angiogenesis were downregulated in the combination-treated CM. Furthermore, we verified that the secretion of VEGF was downregulated and revealed that the reduction might be regulated through the inhibition of the PI3K/AKT pathway. Taken together, our findings demonstrated for the first time that bufalin can enhance anti-angiogenic effect of sorafenib via modulating the AKT/ VEGF signaling pathway.

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Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that PI3K/AKT signaling regulates angiogenesis through affecting the expression of VEGF (9)(10)(11)(12). It may contribute to tumor angiogenesis via a paracrine pathway to the surrounding microvessels by targeting endothelial cells (13,14).…”

Section: Introductionmentioning

confidence: 99%

Bufalin enhances anti-angiogenic effect of sorafenib via AKT/VEGF signaling

Wang

Zhang

Ning

et al. 2016

International Journal of Oncology

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“…[7] Pharmacological studies also report that H. diffusa has anticancer activities. [8][9][10] However, the mechanisms of its anticancer activities are complicated and unclear, which may be related to immunostimulating activity on the immune system, [11] superoxide burst, and caspase activation according to the present studies. [12] Metabolomics, as a multicomponent analysis of tissues or biofluids for organic low-molecular metabolite, provides a novel insight on pharmacology, pathology, toxicology, and genetics, [13][14][15] which enabled its rapid development in disease diagnosis, toxicity screening, drug safety, and efficacy assessment.…”

Section: Introductionmentioning

confidence: 92%

“…Samples of 24-hr period urine from each animal were collected on Day 0 (the day before inoculation) and on Days 2,4,6,8,10,12, and 14 (the days after inoculation), during which the rats were deprived of food for 12 hr to avoid solid debris pollution, but were allowed tap water ad libitum. Fourteen days after tumor inoculation, the animals were sacrificed without an overnight fast, their blood was collected from the ophthalmic vein into tubes containing ethylenediaminetetraacetic acid (EDTA), and tumors were resected and weighed.…”

Section: Animal Handling and Sample Collectionmentioning

confidence: 99%

Metabolic effects of Hedyotis diffusa on rats bearing Walker 256 tumor revealed by NMR‐based metabolomics

Wang

Gao

et al. 2017

Magnetic Reson in Chemistry

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Hedyotis diffusa, a traditional Chinese herbal medicine, is widely used for oncotherapy and shows a positive effect in the clinical treatment. But its mechanism of anticancer activities is complicated and unclear. This study was undertaken to assess the therapeutic effects and reveal detailed mechanisms of H. diffusa for oncotherapy. A Walker 256 tumor-bearing rat model was established, and metabolomic profiles of plasma and urine were obtained from 1 H NMR technique. Multivariate statistical analysis methods were used to characterize the discriminating metabolites between control (C), Walker 256 tumorbearing rats model (M), and H. diffusa treatment (H) groups. Finally, 13 and 10 metabolomic biomarkers in urine and plasma samples were further identified as characteristic metabolites in M group, whereas H group showed a partial metabolic balance recovered, such as ornithine, N-acetyl-L-aspartate, L-aspartate, and creatinine in urine samples, and acetate, lactate, choline, L-glutamine, and 3-hydroxybutyrate in plasma samples. On the basis of the methods above, we hypothesized H. diffusa treatment reduced the injury caused by Walker 256 tumor and maintained a metabolic balance. Our study demonstrated that this method provided new insights into metabolic alterations in tumor-bearing biosystems and researching on the effects of H. diffusa on the endogenous metabolism in tumor-bearing rats.

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“…It has been commonly consumed for health maintenance and also as a dietary medicine for the treatment of prostate cancer [2]. Scientific studies also showed that Hedyotis diffusa WILLD possessed anti-cancer property, and its effects were related to the stimulation of the immune system, activation of caspase and burst of superoxide [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%