Anticancer activity of cissampelos pareira against dalton′s lymphoma ascites bearing mice

Abstract

Background:Cissampelos pareira (Menispermaceae) is used in folk Indian system of alternative medicine, for its analgesic, antipyretic, diuretic, antilithic, and emmenagogue properties.Objective:To evaluate Cissampelos pareira (C. pareira) for in vitro cytotoxicity and in vivo antitumor activity against Dalton's Lymphoma Ascites (DLA) cells in Swiss mice.Materials and Methods:Cissampelos pareira was successively extracted using different solvents. In vitro cytotoxicity was assessed by the MTT assay. An in vivo study was carried out in methanol extract. Twenty-four hours after intraperitoneal inoculation of the DLA cells in mice, the methanol extract of C. pariera (MECP) was administered at 200 and 400 mg/kg body weight for 14 consecutive days. On day 14, six mice were sacrificed and the rest were kept alive for assessment of increase in life-span. The antitumor effect was assessed by evaluating the packed cell volume, viable tumor cell count, increase in body weight, and increase in life-span. The hematological and serum biochemical parameters and anti-oxidant properties were assessed by estimating the superoxide dismutase (SOD), catalase (CAT), and lipid peroxidation.Results:Methanol Extract of Cissampelos pariera (MECP) showed a potent cytotoxic activity, with an IC50 value of 95.5 μg/ml and a significant (P < 0.001) decrease in packed cell volume, viable cell count, and an increased lifespan (54 and 72%). The hematological and serum biochemical profiles were restored to normal levels in MECP-treated mice. The MECP-treated group significantly (P < 0.001) decreased SOD, lipid peroxidation, and CAT to normal.Conclusion:This study demonstrated that C. pariera exhibited significant in vitro and in vivo anti-tumor activities and that it was reasonably imputable to its increasing endogenous mechanism of antioxidant property.

Metabolite Profiling and Quantitation of Cucurbitacins in Cucurbitaceae Plants by Liquid Chromatography coupled to Tandem Mass Spectrometry

“…Cucurbitacins E, I and Q have been shown to possess antitumor and antidiabetic activities 35–40 . C. pareira contains a substantial amount of cucurbitacin Q as found in our study and this concurs with the antiproliferative potential of the plant 30,41 . This does not signify that the anticancer potential of this plant is only due to the presence of a large amount of cucurbitacin Q as it requires further studies.…”

“…This plant is rich in alkaloids and finds some uses in the Indian and Chinese medicine. Many alkaloids from this plant exhibit cytotoxic 23,30 , anti-inflammatory 20 , antiplasmodic activities and this is why it has gained some attention as a natural remedy for malaria in Kenya due to its antimalarial properties 31 . Although this plant is well-known for its alkaloidal content, our research group recently isolated cucurbitacins F and Q from this plant.…”

Cissampelos pareira ethanolic extract modulates hormonal indices, lipid profile, and oxidative parameters in transient infertility-induced female albino rats

“…They found that at 200 and 400 mg/kg dose levels of C. pareira, cholesterol level declined and the triglyceride level was elevated (Surendran et al, 2011). Meanwhile, Thavamani et al (2014) reported that the methanolic extract of C. pareira decreased both cholesterol and triglyceride concentrations in Dalton's lymphoma ascites bearing mice.…”

In Vitro Anticancer Activity of Staphyloxanthin Pigment Extracted from Staphylococcus gallinarum KX912244, a Gut Microbe of Bombyx mori

“…Cancer is a major health issue in almost every part of the world. There is a need for development of new effective anticancer agents as the cancer treatment methods like chemotherapy and radiation therapy pose several side effects [1]. Hence, presently, natural products have been explored and investigated for the development of potent anticancer agents bearing potent antioxidant activity and minimum toxicity to the host cell [2].…”