Anticancer Activity of Jatrophone an Isolated Compound from jatropha gossypifolia Plant Against Hepatocellular Cancer Cell HEP G2 1886

Sukohar, Asep; Herawati, Hening; Gema, Pulido-Cortijo; Setiawan, Gigih; Widya, Morfi Chicy; Sahidin, _

doi:10.13005/bpj/1154

Cited by 12 publications

(5 citation statements)

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“…Despite the few studies that reported the in vitro antiproliferative activity of jatrophone, its cytotoxicity was potent, and in a concentration-dependent way, displaying IC 50 values < 10 µM [ 10 , 13 ]. Herein, we demonstrated for the first time that jatrophone showed potent cytotoxicity against MCF-7 ADR resistant breast cancer cells with an IC 50 value of 1.8 ± 0.2 µM.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, a panel of cancer cell lines representing a range of cancers, including lymphocytic leukaemia, cervical, prostate, and colon cancer, were tested against various fractions of Jatropha species [ 9 – 12 ]. It should be noted that only a small number of studies on JA itself have demonstrated its anticancer activity in vitro against hepatocellular cancerous cells [ 13 ], glioma [ 14 ] and triple-negative breast cancer [ 15 ], and the mechanism of this activity is still elusive and undefined.…”

Section: Introductionmentioning

confidence: 99%

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Jatrophone: a cytotoxic macrocylic diterpene targeting PI3K/AKT/NF-κB pathway, inducing apoptosis and autophagy in resistant breast cancer cells

Shari,

El Gedaily,

Allam

et al. 2023

BMC Complement Med Ther

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Background Breast cancer is a prevalent malignant tumor that affects women worldwide. The primary challenge in treating breast cancer is combating drug resistance, which contributes to relapse and metastasis. Jatrophone is a unique macrocyclic jatrophane diterpene found in various Jatropha and Euphorbia species. It possesses diverse biological and pharmacological activities, including anticancer activity. However, it is unclear whether jatrophone can overcome drug resistance in breast cancer. Methods This study includes the investigation of the cytotoxicity of jatrophone on doxorubicin-resistant breast cancer cells (MCF-7ADR) and the underlying molecular mechanisms. The effects of jatrophone on cell viability were determined using the sulforhodamine B (SRB) assay, while flow cytometry was used to evaluate cell cycle progression, apoptosis, and autophagy. A scratch assay was conducted to observe cell migration, and western blotting was used to measure downstream protein levels (PI3K, AKT, and NF-κB). Unpaired Student’s t-tests were used for comparison between the two groups and the results were analyzed by one-way ANOVA with Tukey- Kremer post hoc test. Results It was shown that jatrophone exhibited potent cytotoxic activity on MCF-7ADR cells in a dose-dependent manner, with an IC50 value of 1.8 µM. It also significantly induced cell cycle S and G/M phase arrest. Interestingly, jatrophone induced both early and late apoptotic cell death, as well as autophagic cell death, with negligible necrosis. Furthermore, jatrophone treatment diminished the migration of MCF-7ADR cells. At the molecular level, jatrophone treatment significantly down-regulated the expression levels of PI3K, AKT, and NF-κB. β. Conclusions The results of the study suggest that jatrophone decreases the proliferation of MCF-7/ADR cells at a low micromolar concentration; induces cell cycle arrest; promotes apoptotic, and autophagic cell death; inhibits migration and EMT; and works on resistance by a mechanism involving the inhibition of the PI3K/Akt/ NF-κB pathway. These findings provide evidence of the potential of jatrophone to be a promising lead compound for targeting doxorubicin-resistant breast cancer cells and could be further investigated for its clinical application as a chemotherapy adjuvant.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Jatrophone: a cytotoxic macrocylic diterpene targeting PI3K/AKT/NF-κB pathway, inducing apoptosis and autophagy in resistant breast cancer cells

Shari,

El Gedaily,

Allam

et al. 2023

BMC Complement Med Ther

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“…Alkaloids in jatropha also called jatrophine could also kill cancer cells. 18 The morphological characteristics of cell necrosis are characterized by cell swelling, rupture of the plasma membrane, the disintegration of the cell membrane structure which causes loss of the cell's ability to survive. A change in the cell nucleus by loss of cell integrity due to damage to the cell membrane is characterized by shrinking, dense, nuclei that have irregular boundaries and become very basophilic (dark in colour).…”

Section: Discussionmentioning

confidence: 99%

Effect of pup out® on caspase 3 and interleukin-1B expression in pulp teeth: a paste contained jatropha, sidaguri, and melittin

Tanumiharja¹,

Hamid²,

Trilaksana³

et al. 2021

J Dentomaxillofac Sci

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Objective: To examine the expression of caspase-3 and IL-1β following application of Pulp Out® on rabbit's pulp teeth as well as histopathology. Material and Methods:The study was conducted on the maxillary incisors rabbits. The teeth were prepared and Pulp Out® was inserted at the base of prepared cavity, then restored with RM-GIC. After 24 hours, the animals were then euthanized and processed for histopathology and immunohistochemistry evaluation. Results:Our results indicated that Pulp Out®, when administered to the pulp teeth, increased the number and diameter of blood vessels. The expression of caspase-3 and IL-1β showed similarities to the expression of commercial devitalizing agent. Conclusion: Our results revealed that Pulp Out® is a valuable devitalization agent that should be further explored for its safety before clinical application.

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“…Utmost notable pharmacological exercises of J. gossypiifolia includes anti-neoplasic activity that often times connected with substance of lignose and terpenoids. The primary reports was delivered by one of the researchers, when creators ethanolic extricate from roots, just to confined diterpene jatrophone, displayed huge restrictory movement in-vitro against cells got from human cancer cells from naso-pharynx and lymphocytic leukemia and in vivo against quaternary standard creature voids frameworks, for example, sarcoma 180 and Walker 259 intramuscular carcinosarcoma [32]. Afterward, triple naive antitumor subordinates of jatrophone secluded via oil ether removes through underlying foundations of J. gossypiifolia.…”

Section: Antineoplastic Properties and Actionmentioning

confidence: 99%

Review on Medicinal Properties of Jatropha gossypiifolia L.

Pande

Trivedi

Kumar

2021

JPRI

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Jatropha gossypiifolia L. broadly addressed as bellyache bush, refers to therapeutic plant utilized all through Asian countries. A few human and veterinary utilizations in conventional medication are portrayed for various specifics and arrangements dependent on aforementioned plant. This paper give a cutting-edge review of conventional uses, pharmacology, and toxicology of J. gossypiifolia species, taking into account examination of its therapeutic worth and potential application in integral and elective medication. Pharmacological examinations have exhibited noteworthy activity of various concentrates elements as anti-microbial, mitigating, anti-diarrheal, anti-hypertensive, and anti-cancerous specialists, among different, supporting a portion of famous employments. No clinical preliminary was distinguished to date. Moreover investigations are important to test significant society utilizations, just as to discover naive bioactive atoms with pharmacological pertinence dependent on the well-known cases. Toxicological investigations related with phytochemical examination are critical to comprehend the possible lethal impacts that could lessen its restorative worth. The current review gives bits of knowledge to future research focusing on both ethnopharmacological approval of its famous use and its investigation as another wellspring of home grown medications and additionally bioactive characteristic items.

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Anticancer Activity of Jatrophone an Isolated Compound from jatropha gossypifolia Plant Against Hepatocellular Cancer Cell HEP G2 1886

Cited by 12 publications

References 0 publications

Jatrophone: a cytotoxic macrocylic diterpene targeting PI3K/AKT/NF-κB pathway, inducing apoptosis and autophagy in resistant breast cancer cells

Jatrophone: a cytotoxic macrocylic diterpene targeting PI3K/AKT/NF-κB pathway, inducing apoptosis and autophagy in resistant breast cancer cells

Effect of pup out® on caspase 3 and interleukin-1B expression in pulp teeth: a paste contained jatropha, sidaguri, and melittin

Review on Medicinal Properties of Jatropha gossypiifolia L.

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