Anticancer Properties of Highly Purified l-Asparaginase from Withania somnifera L. against Acute Lymphoblastic Leukemia

Oza, Vishal P.; Parmar, Pritesh; Kumar, S. Ashok; Subramanian, R. B.

doi:10.1007/s12010-009-8667-z

Cited by 64 publications

(34 citation statements)

References 27 publications

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“…Moreover, alkaline pH (8.0-10) showed optimum pH for most bacterial L-asparaginases activity [60,61]. It has been reported that most plant L-asparaginases have its maximum activity in alkaline pH, and was determined in pH range of 7.5, 8.0 and 8.5 due to the balance between L-aspartic acid and L-aspartate [11,58]. In general most plants show maximum enzyme activity at or near neutral pH [60].…”

Section: Effect Of Phmentioning

confidence: 99%

“…L-asparaginase was detected in different plant species used as a source of protein for human food [4,9]. Recently, several investigators search some plant sources for L-asparaginase production to meet the increase continuous need for L-asparaginases [10][11][12][13]. L-asparagine is the most abundant metabolite for the storage and transport of nitrogen that is utilized in protein biosynthesis [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…L-asparaginase used as chemotherapeutic agent for treatment of human cancer and acts as a catalyst in the breakdown of asparagine, as nutritional requirement for both normal and cancer cells, to aspartic acid in vitro and in vivo [21,24,25]. L-asparaginase from plants origin leads to the death of leukaemic and tumor cells due to catalyses the hydrolysis of asparagine to aspartic acid [6,11,23]. L-asparaginase was used to treat hepatocellular cell lines in vitro and in vivo to examine the therapeutic efficacy [22,26,27].…”

Section: Introductionmentioning

confidence: 99%

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Anticancer Activity of L-Asparaginase Produced from Vigna Unguiculata

Moharib

2018

World Scientific Research

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The present study aimed to production of purified L-asparaginase from Vignaunguiculata. Different physiological parameters, such as pH, temperature and incubation period, were optimized for growth and maximum L-asparaginase production. The optimum parameters were 37°C, 30 min and pH 8.5. Maximum L-asparaginase was 886.4U/ml with a specific activity of 1140.7 U/ml (31 fold purification with 28 %yield) were obtained at optimum conditions. The purified L-asparaginase produced from Vignaunguiculata was used for characterization and general properties. The effect of pH and temperature on L-asparaginase activity as well as stability at different pH and temperature were determined. The optimum pH 8.5 and 37ºC temperature on L-asparaginase showed 100% residual activity. Stability of pH around 8.5 and temperature 70ºC showed 90 and 78 % residual activity at 30 and 60 min respectively. The Lasparaginase showed high stability at alkaline pH (pH 8.5) when incubated for up to 60h.The molecular weight of the produced L-asparaginase was close to 68.5 kDa. Cytotoxic activity of Lasparaginase was examined in vitro using four carcinoma cell lines. L-aspargenase has higher effective in growth inhibition against HEPG2 and HCT-116 but lower against HELLA and MCF7 carcinoma cell lines. The data show that L-aspargenase has a higher cytotoxic activity against HEPG2 and HCT116, revealed higher percentage of cell death, indicating antitumor properties, and demonstrate direct effect on cancer cell proliferation of HEPG2 and HCT116. Therefore, Vignaunguiculata was considered to be a suitable source for production of Lasparaginase has higher activity and good stability. Purified L-asparaginase obtained from Vignaunguiculata could be employed in drug chemotherapy and treatment of cancer.

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Section: Effect Of Phmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anticancer Activity of L-Asparaginase Produced from Vigna Unguiculata

Moharib

2018

World Scientific Research

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“…Although the amino acid sequence of plant L-asparaginase is not similar to bacterial enzyme but it is 66% similar and 23% identical to human glycosylasparaginase 11 . The plant L-asparaginase is less toxic as compared to bacterial L-asparaginase 12 .…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of L-Asparaginase Extracted From Plant and Microbial Sources

Kaur¹,

Kumar²,

Kumar³

2017

Int. J. Res. Ayurveda Pharm.

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L-asparaginase has been used for the treatment of different leukemia such as Acute Lymphoblastic Leukemia. Unlike normal cells leukemic cells cannot synthesize asparagine on their own. Therefore, when L-asparaginase is injected into blood it breaks down asparagine to aspartic acid and ammonia and hence tumor cells die due to asparagine starvation. It is produced by both microbes as well as plants. Therefore, in the present study we compared the L-asparaginase extracted from plants as well as microbes. Among the plants S. nigrum showed the highest activity (52 IU/ml) whereas among microbes B. subtilis (MTCC-121) had the highest activity (43.11 IU/ml). Both the enzymes were kinetically characterized and it was found that plant enzyme was stable over pH and temperature variations. The effect of metal ions was same on both the enzymes whereas chelators enhanced the plant enzyme whereas had no effect on microbial enzyme. Plant enzyme showed the highest activity and was more stable to the environmental variations as compared to microbial enzyme.

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“…Among these, bacteria-produced Lasparaginase is the most extensively explored, due to their cost-effective nature (Theantana et al, 2009). Many of the commercially-produce L-asparaginase is derived from Escherichia coli, Erwinia carotovora and Serratia marcescens (Oza et al, 2010). L-asparaginase from these prokaryotic sources was however, discovered to cause allergic reactions and anaphylaxis.…”

Section: Introductionmentioning

confidence: 99%

Influence of Glucose and L-Asparagine Concentrations on L-Asparaginase Production by Endophytic Fungi

Chow¹,

Ting²

2017

J microb biotech food sci

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In this study, five L-asparaginase–producing endophytes (ODL4, PBS1, PBL13, CCL1, MKS1) were evaluated for their L-asparaginase production when cultivated in various glucose (0, 0.5, 1, 2, 3, 4, 5 %) and L-asparagine (0, 5, 10, 20, 30, 40, 50 mM) concentrations over a period of 20 days. L-asparaginase production was quantified via Nesslerization and expressed as L-asparaginase activities. Results revealed supplementation with nitrogen-source (L-asparagine) induced higher L-asparaginase activities compared to carbon-source (glucose), with means of 0.106 compared to 0.062 µM mL-1 min-1, respectively. The optimum concentration of L-asparagine was 30 mM. On the contrary, optimum levels for glucose was inconclusive. Among the isolates, isolate MKS1 produced the highest L-asparaginase activities when supplemented with 5% glucose and 10 mM asparagine. This study revealed that nitrogen-based source (L-asparagine) is more effective than carbon-source (glucose) in inducing L-asparaginase activities.

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Anticancer Properties of Highly Purified l-Asparaginase from Withania somnifera L. against Acute Lymphoblastic Leukemia

Cited by 64 publications

References 27 publications

Anticancer Activity of L-Asparaginase Produced from Vigna Unguiculata

Anticancer Activity of L-Asparaginase Produced from Vigna Unguiculata

Comparative Characterization of L-Asparaginase Extracted From Plant and Microbial Sources

Influence of Glucose and L-Asparagine Concentrations on L-Asparaginase Production by Endophytic Fungi

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