Anticandidal activity and biocompatibility of a rechargeable antifungal denture material

Abstract

Objectives Candida‐associated denture stomatitis is a recurrent and debilitating oral mucosal disease. Development of anticandidal denture materials represents a promising strategy to manage this condition. We have previously shown that miconazole incorporated in methacrylic acid (MAA) copolymerized diurethane dimethacrylate (UDMA) denture materials has long‐term anticandidal activity. In this study, we examined the ability of culture medium conditioned with drug‐free‐ or miconazole‐MAA‐UDMA discs to prevent Candida infection in an in vitro oral epithelial cell/Candida albicans coculture system. Materials and Methods Candida albicans (C. albicans)‐induced OKF6/TERT‐2 cell damage was quantified by the release of lactate dehydrogenase from epithelial cells, cytokine production was quantified using protein cytokine arrays, and the expression of C. albicans genes was measured by RT‐qPCR. Results Candida albicans had limited growth with altered expression levels of secreted aspartyl proteinase‐2 and ‐5 in culture medium conditioned by miconazole‐MAA‐UDMA discs. Significantly, the ability of C. albicans to induce oral epithelial cell damage and trigger epithelial proinflammatory cytokine production was also inhibited by miconazole disc conditioned media. Conclusion Miconazole released from MAA‐UDMA denture materials effectively prevents the development of candidal infection in an in vitro oral epithelial system. Further characterization of this drug‐rechargeable denture material is warranted.

N‐trimethylchitosan/Alginate Layer‐by‐Layer Self Assembly Coatings Act as “Fungal Repellents” to Prevent Biofilm Formation on Healthcare Materials

“…In this study, a PMMA‐based polymer, Lucitone 199 (Dentsply Intl, York, PA), was used as a representative, clinically relevant material. PMMA is a versatile polymer with multiple dental and medical applications, including complete denture bases, bone cements, screws for bone fixation, fillers for bone cavities/skull defects, and vertebral stabilization in osteoporotic patients, where fungal biofilm formation has become a growing concern . To introduce anionic groups onto the PMMA surfaces for TMC binding and subsequent TMC/SA LBL coating, methacrylic acid (MAA) was grafted onto the PMMA polymer.…”

“…In our continuing effort to design and fabricate antifungal materials for clinical use, we discovered that the layer‐by‐layer (LBL) self‐assembly of trimethylchitosan/sodium alginate (TMC/SA) multilayer surface coatings on various clinically relevant biomaterials effectively prevented fungal biofilm formation. TMC is one of the most widely used water‐soluble cationic derivatives of chitosan.…”

Lectin-Conjugated Liposomes as Biocompatible, Bioadhesive Drug Carriers for the Management of Oral Ulcerative Lesions

“…The human keratinocyte cell line (OKF6/TERT-2) used to model oral epithelial cells was a gift from Dr. Cris Villar. 27 As mentioned above, this cell line consists of immortalized human oral epithelial cells. The cells were cultured at 37 °C in a humidified 5% CO 2 incubator in a keratinocyte-serum-free medium supplemented with human recombinant epidermal growth factor (EGF 1-53), bovine pituitary extract (BPE), fetal bovine serum, hydrocortisone, and antibiotics purchased from Gibco by Life Technologies, Thermo Fisher Scientific (Waltham, MA, USA).…”

Rechargeable anticandidal denture material with sustained release in saliva

“…Some of these new materials have shortcomings that limit their usefulness in the clinic. To address these deficiencies, we recently described the fabrication and characterization of a new acrylic-based denture material (PMMA-g-PNVP) that provides sustained antifungal release for 30 or more days and can be charged ('click on') and discharged ('click off') with different antifungal drugs so that treatment can be tailored to the clinical needs of the patient Villar et al 2013).…”