Anticandidal and synergistic effect of essential oil fractions from three aromatic plants used in Cameroon

Ngo-Mback, M.N.L.; Babii, Cornelia; Dongmo, Pierre Michel Jazet; Toghueo, Rufin Marie Kouipou; Ștefan, Marius; Boyom, Fabrice Fekam

doi:10.1016/j.mycmed.2020.100940

Cited by 13 publications

(15 citation statements)

References 26 publications

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“…The interference of BrCl-flav with the cell wall was assessed using the microdilution method presented above for determination of MIC. The MIC was recorded in the absence and presence of 0.8 M sorbitol after 48 h and it was considered as the lowest concentration at which no visible growth was observed [6].…”

Section: Sorbitol Binding Affinity Assaymentioning

confidence: 99%

“…The effect of BrCl-flav on C. albicans, C. krusei and C. glabrata biofilm-forming ability was determined as previously described [6]. Briefly, 250 µL of a cell suspension (1 × 10 3 CFU/mL) in SDB was added into each well of a microtiter plate.…”

Section: In Vitro Anti-biofilm Activity Assaymentioning

confidence: 99%

“…to form biofilms on different surfaces [5]. Due to their resistance to multiple drugs, biofilm cells survive to antifungals concentrations 1000-fold higher than those which inhibit planktonic cells [6]. Therapeutical limitations caused by multidrug resistance phenomenon make the control of Candida infections a major challenge for modern medicine.…”

Section: Introductionmentioning

confidence: 99%

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The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-Candida Activity by Damaging Cell Membrane Integrity

et al. 2021

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Infections caused by Candida are very difficult to treat due to increasing antifungal resistance. Recent studies showed that patients with Candida infections resistant to fluconazole have very few treatment options. Therefore, finding new efficient antifungal agents is a matter of medical high priority. The aim of this study was to explore the antifungal potential of BrCl-flav-a representative of a new class of synthetic flavonoids with bromine as halogen substituent at the benzopyran core against four Candida clinical strains. Determination of minimum inhibitory concentration and minimum fungicidal concentration along with the time kill assay indicated a strong antifungal effect of BrCl-flav against C. albicans, C. parapsilosis, C. krusei and C. glabrata. The investigation of anti-Candida mechanism of action using fluorescence microscopy and scanning electron microscopy revealed that Br-Cl flav could inhibit fungal growth by impairing the membrane integrity, the resulting structural damages leading to cell lysis. BrCl-flav also showed important anti-virulence properties against Candida spp., inhibiting biofilm formation and yeast to hyphal transition. A strong synergistic antifungal effect against C. albicans strain was observed when BrCl-flav was used in combination with fluconazole. BrCl-flav has a good potential to develop new effective antifungal agents in the context of Candida spp. multidrug resistance phenomenon.

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Section: Sorbitol Binding Affinity Assaymentioning

confidence: 99%

Section: In Vitro Anti-biofilm Activity Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-Candida Activity by Damaging Cell Membrane Integrity

et al. 2021

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“…A series of sub-inhibitory concentrations of plant extracts were tested against bacterial biofilms formation. A microtitre plate biofilm assay as described in previous works 19,26 with some modifications was used. An amount of 100 μL from overnight cultures of bacteria was added into 100 μL of fresh MHB supplemented with 6% glucose and cultivated in the presence or absence of plant extracts.…”

Section: Inhibition Of Bacterial Biofilms Formationmentioning

confidence: 99%

“…Its greater diversity could explain the highest activity of flower extracts in secondary metabolites than the other extracts. These secondary metabolites could act synergistically and exhibit a potent antibacterial action of the extract 19 . The essential oil has also shown better antibacterial activity on E. coli, S. aureus, K. pneumoniae, and Salmonella than the one obtained by some researchers.…”

Section: Antimicrobial Activity 331 Hydroethanolic Extracts and Essential Oil Inhibitory Concentrationsmentioning

confidence: 99%

Antibacterial activities of the essential oil and hydroethanolic extract from Aeollanthus heliotropioides Oliv

et al. 2021

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To contribute to control bacterial infections, we proposed evaluating the antibacterial activities and modes of action of Aeollanthus heliotropioides essential oil and its hydroethanolic extract. Solvent extracts and essential oil were obtained from the aromatic plant's aerial parts by hydroethanolic maceration and hydro-distillation. The analyses of the chemical composition were performed using gas chromatography coupled with mass spectrometry. The microdilution method evaluated the in vitro antibacterial potential of the essential oil and solvent extracts. The Inhibition of biofilms formation was carried out using a colorimetric biofilm microdilution assay with crystal violet as a dye. The effect of extract and essential oil on the release of nucleic acids was performed using a spectrophotometric method. The time-kill kinetic assay was assessed for hydroethanolic extracts and essential oil. The extraction yield was 0.1%, and the major compounds identified in the essential oil were linalool (43.47%) and cis-α-farnesene (42.67%). The phytochemical screening revealed flavonoids, saponins, phenols, triterpenes, catechin tannins, and quinones. Minimum Inhibitory Concentrations (MICs) ranged from 2.08 mg/mL to 10 mg/mL. Concerning the modes of action, the essential oil showed its bactericidal effect at 2 hours. The reduction of Escherichia coli biofilms formation was found at 0.21 mg/mL. The essential oil treatments resulted in a release of nucleic acids at a concentration of 2.1 mg/mL. These results justify using the essential oil and hydroethanolic extracts of Aeollanthus heliotropioides as a potential source of molecules with antibacterial activity.

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Antifungal Properties of Essential Oils Derived from the Genus Cymbopogon: A Systematic Review

Butzge,

Pivotto,

Mezzomo

et al. 2023

Chemistry & Biodiversity

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Essential oils (EOs) are products of secondary metabolism with recognized organoleptic characteristics and biological properties. Recently, there has been a growing demand for EOs in the national and international market, mainly due to the recognition of their use as complementary medicine practices, and the increased use in the industries of pharmaceutics, cosmetics, well‐being, veterinary and agroecology, boosting the productive sector. In this context, EOs from grasses of the Cymbopogon (Poaceae) are promising sources of bioactive compounds, due to their recognized biological properties, such as anti‐inflammatory, antibacterial, antifungal, antidiabetic, repellent, and larvicide. Thus, the present study aims to carry out a review of the scientific literature of the main works related to the evaluation of the antifungal action of essential oils extracted from plants of the Cymbopogon genus, compiling the species that showed the best results and relating them to their main chemical constituents. This review covers the following species: C. citratus, C. flexuosus, C. winterianus, C. martinii, C. nardus, C. giganteus, C. schoenanthus, C. khasans, and C. proximus. Among them, C. citratus was the most assessed, being associated with the vast majority of studies (61.9%), and it was also the species that showed the best results in terms of MIC.

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Anticandidal and synergistic effect of essential oil fractions from three aromatic plants used in Cameroon

Cited by 13 publications

References 26 publications

The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-Candida Activity by Damaging Cell Membrane Integrity

The Antibacterial Synthetic Flavonoid BrCl-Flav Exhibits Important Anti-Candida Activity by Damaging Cell Membrane Integrity

Antibacterial activities of the essential oil and hydroethanolic extract from Aeollanthus heliotropioides Oliv

Antifungal Properties of Essential Oils Derived from the Genus Cymbopogon: A Systematic Review

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