Anticarcinogenic Effect of Tetrachlorodecaoxide after Total-Body Gamma Irradiation in Rats

Abstract

Tetrachlorodecaoxygen (TCDO) therapy of acute radiation syndrome was tested for a possible influence on the development of X-ray-induced malignancies. BD IX rats were exposed to total-body irradiation (TBI, gamma rays, 9 or 11 Gy) and received daily intravenous injections of either TCDO or physiological saline solution from days 4 through 11 after TBI. The short-term TCDO therapy reduced the acute death rate markedly, but survival rates after 4 months were similar with and without TCDO. The first malignancy after TBI occurred on day 103, and over the lifetime of the animals the tumor incidence in the group given TBI (11 Gy) without TCDO treatment was 73% vs 20% in animals with short-term TCDO therapy after TBI. In particular, there was a highly significant prevention of radiation-induced leukemia [P (one-sided) < 0.001] by TCDO, and a significantly reduced incidence of malignant epithelial tumors [P (one-sided) < 0.05]. The development of sarcomas was not affected by TCDO. Long-term survival was not enhanced by TCDO due to the occurrence of bronchopneumonial infections about 1 year after TBI. In conclusion, TCDO is not only a potent therapeutic agent in acute radiation syndrome, but it also significantly reduced the carcinogenic risk in rats after exposure to ionizing radiation.

A randomized controlled phase IIb wound healing trial of cutaneous leishmaniasis ulcers with 0.045% pharmaceutical chlorite (DAC N-055) with and without bipolar high frequency electro-cauterization versus intralesional antimony in Afghanistan

“…Within the present randomized controlled three-armed clinical trial we investigated the benefit of applying local wound treatment to OWCL ulcers. The overall aim of this RCT phase IIb trial in Mazar-e-Sharif, Afghanistan, was (a) to confirm previous results [ 12 ] using bipolar HF-EC combined with subsequent moist wound treatment (MWT) with 0.045 % of the pharmaceutical sodium chlorite solution (DAC N-055); and to directly compare the results (b) with topical anti-parasitic SSG and (c) with 0.045% DAC N-055 MWT alone, known to promote tissue regeneration [ 3 ],[ 5 ],[ 6 ]. The trial was further encouraged by the previous successful treatment of four patients with facial lupoid leishmaniasis, in whom the topical application of 0.045% DAC N-055 led to a rejuvenation of the faces [ 17 ],[ 18 ].…”

“…Liquid nitrogen, the CO 2 laser, bipolar HF-EC, PDT, and pharmaceutical chlorite applied to the wound [ 3 ],[ 5 ],[ 6 ] or to the skin [ 17 ],[ 18 ], where NaClO 2 reacts with heme or bacterial dismutases, are likely to have a common denominator for the of wound healing, which might be 1 O 2 or other ROS.…”

“…If not further concentrated under vacuum (which is commonly practiced with industrial NaClO 2 ), pharmaceutical chlorite contains a chlorine peroxide contaminant, formerly called tetradecachlorooxygen (TCDO), a chemical name refuted by the German Health Authorities [ 4 ]. The chlorine peroxide seems to be important for systemic regenerative effects in stem cell compartments of rats [ 5 ],[ 6 ]. Recent advances in bacterial heme protein biochemistry [ 7 ],[ 8 ] have reformed our understanding of chlorite biochemistry [ 9 ] showing that in vivo reactions of chlorite with heme analogues can either produce hypochlorite, which in turn can react with H 2 O 2 to form singlet oxygen 1 O 2 , or which can dismutate to Cl - and 1 O 2 .…”

WF10 Stimulates NK Cell Cytotoxicity by Increasing LFA‐1‐Mediated Adhesion to Tumor Cells

“…WF10 was shown to inhibit the development and promotion of leukemia in animal models [11, 12]. The role of oxidative species in cancer is complex and has been extensively studied in the last decades.…”

“…WF10 is known to have various immunological effects by stimulating innate immune functions, while inhibiting adaptive immune functions [10]. In contrast to control animals, sublethally irradiated rats treated with WF10 developed significantly less hematological or solid tumors than controls [11, 12]. This antitumor effect of WF10 could be explained by stimulating the innate natural cytotoxicity against tumors.…”