Anticentromere antibodies are the most potent antinuclear antibodies in reducing live birth outcomes after ICSI

Teramoto, Shokichi; Ueno, Tsuyoshi; Aono, Fumihito; Okubo, Tsuyoshi; Segawa, Tomoya; Osada, Hisao; Shozu, Makio

doi:10.1016/j.rbmo.2024.103864

Reproductive BioMedicine Online

2024

DOI: 10.1016/j.rbmo.2024.103864

|View full text |Cite

Anticentromere antibodies are the most potent antinuclear antibodies in reducing live birth outcomes after ICSI

Shokichi Teramoto,

Tsuyoshi Ueno,

Fumihito Aono

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Anti‐Centromere Protein A Antibody Disrupts the Competence of Mouse Oocytes Matured In Vitro

Shioya,

Takahashi,

Nakano

et al. 2024

American J Rep Immunol

View full text Add to dashboard Cite

IntroductionAnticentromere autoantibodies are associated with refractory IVF/ET failure, but causality is unclear. Experimental models are needed.MethodsImmature oocytes collected from 23‐day‐old mice were matured in vitro for 18 h in a culture medium containing an anti‐human centromere protein A (CENP‐A) polyclonal antibody, and those oocytes’ maturity and chromosome/spindle structure were assessed.ResultsAntibody exposure did not affect the germinal vesicle breakdown ratio but reduced the first polar body formation ratio by 13% at the highest concentration (70.0 µg/mL). Metaphase II (MII) oocytes were stained for chromosomes/spindles and grouped into aligned/barrel‐like (AB), scattered/weakly‐stained (SW), and condensed/absent (CA). Antibody exposure decreased AB and increased SW and CA in a dose‐dependent manner. The AB/SW/CA percentages were 86/14/0, 86/14/0, 35/65/0, and 0/0/100 in the 0, 17.5, 35.0, and 70.0 µg/mL antibody groups, respectively (underlined values represent p < 0.05 compared with 0 µg/mL). Next, metaphase II oocytes were subjected to intracytoplasmic sperm injection, and the number of pronucleus/pronuclei (PN) was counted 6 h later. Antibody exposure decreased two pronuclei oocytes and increased non‐two pronuclei oocytes dose‐dependently. The percentages of 0/1/2/3 pronuclei oocytes were 43/0/57/0, 37/0/21/42, 16/28/48/8, and 91/4/4/0 in the 0, 17.5, 35.0, and 70.0 µg/mL groups, respectively.ConclusionsAnti‐CENP‐A antibody impaired a linear alignment of chromosomes at metaphase II and enhanced one or three PN formation after ICSI, which are similar to findings reported for infertile women with anticentromere autoantibodies.

show abstract

Anti‐Centromere Protein A Antibody Disrupts the Competence of Mouse Oocytes Matured In Vitro

Shioya,

Takahashi,

Nakano

et al. 2024

American J Rep Immunol

View full text Add to dashboard Cite

show abstract

The Association of ACA on Embryonic Development and Pregnancy Outcomes in Patients Undergoing IVF/ICSI Treatment

Lian,

Zhang,

Liu

et al. 2024

American J Rep Immunol

View full text Add to dashboard Cite

ObjectiveTo investigate whether anti‐centromere antibody (ACA) is associated with embryonic development and clinical outcomes in patients undergoing IVF/ICSI treatment.Materials and MethodsWomen who underwent fresh or frozen embryo transfer cycles at a reproductive center between January 1, 2017 and January 1, 2023, were retrospectively studied. Women were divided into ACA– group and ACA+ group according to ACA levels. After controlling the bias between groups using the propensity score‐matching (PSM) method, the impact of ACA on embryonic development and clinical outcomes was compared between the two groups. Propensity score regression adjustment and inverse probability treatment weighting were used for sensitivity analysis.ResultsA total of 18 578 patients with ACA screening information were included in the analytical sample, with 164 patients (0.88%) diagnosing ACA positive. After 1:3 PSM, 656 patients (492 in the ACA– group, 164 in the ACA+ group) were finally included. There were significant differences in the rates of cleavage, available embryo, blastocyst formation, and high‐quality blastocyst formation between the ACA– group and the ACA+ group. Linear regression analysis indicated that ACA was negatively associated with the rates of available embryos on Day 3, blastocyst formation, and high‐quality blastocyst formation after adjusting potential confounding factors. Binary logistic regression analysis suggests that ACA positivity does not affect clinical outcomes after IVF/ICSI treatment.ConclusionsIn this cohort study of patients undergoing IVF/ICSI treatment, ACA positivity significantly interfered with embryonic development. These findings suggest that ACA evaluation before IVF/ICSI treatment might be a promising biomarker for predicting embryonic development but not for clinical outcomes.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Anticentromere antibodies are the most potent antinuclear antibodies in reducing live birth outcomes after ICSI

Cited by 2 publications

References 29 publications

Anti‐Centromere Protein A Antibody Disrupts the Competence of Mouse Oocytes Matured In Vitro

Anti‐Centromere Protein A Antibody Disrupts the Competence of Mouse Oocytes Matured In Vitro

The Association of ACA on Embryonic Development and Pregnancy Outcomes in Patients Undergoing IVF/ICSI Treatment

Contact Info

Product

Resources

About