Anticestodal properties of Hibiscus rosa-sinensis L. (Malvaceae): an in vitro and in vivo study against Hymenolepis diminuta (Rudolphi, 1819), a zoonotic tapeworm

Nath, Purobi; Yadav, Arun K.

doi:10.1007/s12639-015-0664-2

Cited by 10 publications

(8 citation statements)

References 14 publications

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“…Regarding the anthelmintic studies on medicinal plants it emerges that various previous workers have assessed the anthelmintic potentials of different medicinal plants using either in vitro assays (Vijaya and Yadav 2014) or in vivo models (Tangpu et al 2004;Yadav and Temjenmongla 2012). However, it is only in few cases that an attempt has been made to employ both in vitro and in vivo tests to comment upon precisely the anthelmintic effects of a particular medicinal plant (Nath and Yadav 2015).…”

Section: Discussionmentioning

confidence: 99%

Therapecutic efficacy of the leaf extract of Croton joufra Roxb. against experimental cestodiasis in rats

Gogoi

Yadav

2016

J Parasit Dis

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The leaves of have been used as a traditional medicine in Assam (Northeast India) to treat the intestinal helminthic infections. The aim of this study was to establish the in vitro and in vivo anthelmintic efficacy of the leaf extract of this plant on, a zoonotic tapeworm. The plant extract was also screened for its chemical constituents. The in vitro anthelmintic efficacy of the plant was evaluated by physical motility test on adult cestodes, following their exposure to 10, 20, 30 mg/ml concentrations of the crude leaf extract, while the in vivo effect was determined based on the eggs per gram (EPG) counts and worm recovery rates, using an experimentally induced model of infections in Wistar rats. The in vitro study results showed a dose-dependent effect on the parasite and the highest concentration of the extract (30 mg/ml) caused mortality of the worms in 1.53 ± 0.12 h as compared to 3.46 ± 0.10 h shown by 1 mg/ml concentration of the reference drug, praziquantel (PZQ). The in vivo results showed that a single 800 mg/kg dose of the extract, given for 3 days, was highly efficacious against adult parasites as it reduced the EPG count and worm burdens of rats by 94.74 and 75 %, respectively. The extract also showed almost similar degree of effects against the larval stage of the parasite. As ascertained by the qualitative screening tests, the leaf extract of contains terpenoids, tannins, alkaloids, flavonoids, glycosides and reducing sugar. The overall findings of this study suggest that leaf extract possesses significant anthelmintic efficacy and support its traditional anthelmintic claims.

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Section: Discussionmentioning

confidence: 99%

Therapecutic efficacy of the leaf extract of Croton joufra Roxb. against experimental cestodiasis in rats

Gogoi

Yadav

2016

J Parasit Dis

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“…In a recent study, Acorus calamus Linn. and Hibiscus rosa-sinensis L., showed significant anthelmintic activity against the cestode parasite, Hymenolepis diminuta [9,10]. Cynodon dactylon L. Pers and Elaeocarpus floribundus also showed anthelmintic efficacy against Hymenolepis diminuta, both in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical, Antioxidant, and Trace Element Analysis of Anthelmintic Plants of North-East India

Swargiary

Nath

Basumatary

et al. 2017

Int J Pharm Pharm Sci

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Objective: Cynodon dactylon, Hibiscus rosa-sinensis, Elaeocarpus floribundus and Acorus calamus are anthelmintic medicinal plants of north-east India. Due to its pharmacological properties, the present study was aimed to investigate the phytochemical, antioxidant and heavy metal content of the plants.Methods: Preliminary phytochemical screening was carried out for the presence of phenolics, flavonoids, proteins, and carbohydrates following standard protocols. The antioxidant potential of plants was studied by phosphomolybdate, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay. The heavy metal content was estimated using atomic absorption spectrophotometer (Perkin Elmer 3110). Results:The result showed that the alcoholic extracts of the plant sample contain high quantity of proteins, carbohydrates, phenolics, and flavonoids. Antioxidant assays revealed appreciable free radical scavenging potential. The total antioxidant activity was highest in E. floribundus (62.48±1.53 µg/mg extract) while C. dactylon showed lowest (34.00±0.32 µg/mg extract). Similarly, DPPH and FRAP study also showed high in E. floribundus and lowest in H. rosa-sinensis and A. calamus. Elemental analysis of the plant extracts showed a high content of Mn in all the plants while Co was found to be lowest. The tested plants showed low levels of toxic elements. Conclusion:The high quantity of nutritional content and antioxidant property suggest that plants may be a good source of phytomedicine. Less quantity of toxic elements offers an opportunity to explore the medicinal application of the plants. However, further study needs to be carried out to know its mode of action.

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“…In vivo assay revealed that at the highest dose, rats infected with H. diminuta, showed a higher reduction of worm and EPG counts as compared to mice infected with S. obvelata. In a similar study by Nath and Yadav (2015), the leaf extract of Hibiscus rosa-sinensis showed promising anticestodal efficacy. Interestingly in a similar study by Gogoi and Yadav (2016), the methanolic extract of Caesalpinia bonducella showed a better in vivo nematicidal efficacy.…”

Section: Discussionmentioning

confidence: 85%

In vitro and in vivo anthelmintic study of Sesbania sesban var. bicolor, a traditionally used medicinal plant of Santhal tribe in Assam, India

2020

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The leaf decoction of Sesbania sesban var. bicolor is used traditionally by Santhal tribe of Assam, India, for the treatment of intestinal helminthic infections. This study was conducted to evaluate the in vitro and in vivo anthelmintic efficacy of methanolic extract of S. sesban var. bicolor leaves using Hymenolepis diminuta-rat (cestode) and Syphacia obvelata-mice (nematode) as test parasites and models. Praziquantel (PZQ) and albendazole (ABZ) were used as reference drugs. At the highest concentration of 30 mg/ml of the plant extract, H. diminuta and S. obvelata showed mortality at 0.81 ± 0.01 h and 15.17 ± 0.05 h, respectively. The in vivo results substantiated the in vitro findings, and the extract showed a better cestocidal efficacy in a dose-dependent manner, whereby treatment of rats with 400 mg/kg of the plant extract caused 65.10% reduction in eggs per gram (EPG) of faeces and 56% reduction in worm counts. S. obvelata-infected mice treated at the same dose showed 34.32% and 47.08% reduction in EPG and worm counts at necropsy, respectively. The methanolic extract was subjected to bioassayguided fractionation using different solvents and the ethyl acetate fraction proved to be the most active. This active fraction was subjected to column chromatography using varying concentrations of hexane:ethyl acetate. Maximum efficacy was observed in 7:3 hexane:ethyl acetate, where H. diminuta and S. obvelata showed mortality at 3.56 ± 0.12 h and 9.21 ± 0.02 h, respectively. This indicates that the isolated fraction contained the active component responsible for its anthelmintic activity, which substantiates the medicinal usage in traditional practice.

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Anticestodal properties of Hibiscus rosa-sinensis L. (Malvaceae): an in vitro and in vivo study against Hymenolepis diminuta (Rudolphi, 1819), a zoonotic tapeworm

Cited by 10 publications

References 14 publications

Therapecutic efficacy of the leaf extract of Croton joufra Roxb. against experimental cestodiasis in rats

Therapecutic efficacy of the leaf extract of Croton joufra Roxb. against experimental cestodiasis in rats

Phytochemical, Antioxidant, and Trace Element Analysis of Anthelmintic Plants of North-East India

In vitro and in vivo anthelmintic study of Sesbania sesban var. bicolor, a traditionally used medicinal plant of Santhal tribe in Assam, India

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