Anticholinesterase Activity and Mass Spectral Analysis of Olea dioica Roxb., An in vitro Study

Pratap, G.; Rather, Suhail Ahmad; Shantaram, Manjula

doi:10.36468/pharmaceutical-sciences.686

Cited by 5 publications

(7 citation statements)

References 33 publications

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“…Flowers blossom from February through April (8). The high antioxidant effectiveness of O. dioica leaf extract was previously reported in a research study (11), as also with its antimicrobial (12), anticholinesterase (13), and analgesic activities (14). A cytotoxic study was reported on the bark and leaves of O. dioica for colorectal and breast cancers using ethanol solvent extract (8,15).…”

Section: Introductionmentioning

confidence: 75%

Augmentation of in vitro cytotoxic potentiality of crude methanolic leaf extract of Olea dioica as a pivotal anticancer source

Krishnappa¹,

Karthik²,

Shantaram³

2023

Biomedicine

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Introduction and Aim: Tumor is the major cause of death world-wide, with an approximate 10 million fatalities in 2020, or almost one in every six deaths. The concerns with the current commercially available anticancer medications' low selectivity and persistent side effects have driven the research of safer and more effective chemotherapeutic drugs. The current work is focused on determining the cytotoxic potential of crude methanolic extract of Olea dioica leaves against various cancer cell lines viz., A549 (human lung cancer), HCT116 (human colorectal cancer), HeLa (human cervical cancer), MCF-7, and MDA-MB-231 (human breast cancer). Materials and Methods: The cells were treated with crude methanolic extract at five different concentrations viz., 25, 50, 100, 250 and 500 µg/ml to assess the cell viability. Results: The results from the assay performed for five cell lines have shown that methanolic extract of O. dioica has an exponential cytotoxicity potential on HeLa cell line followed by MCF-7 (IC50 value 81.05), HCT116 (106.86) and MDA-MB-231 with IC50 value 81.05, 106.86, 141.34 and 499.24 µg/ml respectively, whereas the A549-human lung cancer cell has shown no cytotoxic activity. Conclusion: The methanol extract of the O. dioica leaf could be recognized as a pivotal source of anticancer compounds, but furthermore research is needed to investigate the chemical components of O. dioica extracts that are mainly accountable for anticancer action and to expound more precise molecular processes of cell death.

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Section: Introductionmentioning

confidence: 75%

Augmentation of in vitro cytotoxic potentiality of crude methanolic leaf extract of Olea dioica as a pivotal anticancer source

Krishnappa¹,

Karthik²,

Shantaram³

2023

Biomedicine

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“…Electron impact ionisation was employed (70 eV), and the MS (mass spectrometry) ion source was maintained at 240°C. Compounds were identified by their gas chromatography retention times and mass spectra using standard compounds for comparison with methanolic fraction’s GC-MS graph ( Pratap et al, 2020a ; Pratap et al, 2020b ).…”

Section: Methodsmentioning

confidence: 99%

“…Instruments for LC-MS (Agilent 1100 LC, Bruker-make MS model Esquire 3000) having an ESI (electrospray ionisation), an APCI (atmospheric pressure chemical ionisation), and a PDA detection source with a mass range of 100 amu in quadrupole and 10,000 amu ( Jain et al, 2016 ). The mass-charge ratio and molecular mass of the methanolic fractions were determined by using the spectrum database for organic compounds in MassBank, which was then utilised to identify the mass fragmentations ( Pratap et al, 2020b ; Gk et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…The sample was formed by uniformly dispersing the methanolic fractions within a matrix of KBr ( Owokotomo et al, 2015 ). The intensity bands were compared to standard values in order to identify the functional groups ( Pratap et al, 2020b ; Gk et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…In order to establish the presence of putative AChE inhibition zones, the plates were later dried at room temperature for 1 min, resulting in colourless or white specks on a yellow background. After measuring the Rf value of the plant bioactive components that were calculated for the separation of the molecule in preparative scale, the spots on the plates were confirmed for being possible AChE inhibitors and recognised as clear zones against a yellow background ( Marston et al, 2002 ; Pratap et al, 2020a ; Pratap et al, 2020b ).…”

Section: Methodsmentioning

confidence: 99%

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In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Kenchappa,

Karthik,

Vijendra

et al. 2023

Front. Pharmacol.

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Alzheimer’s disease (AD) is a type of neurodegenerative disease, associated with the hastening of ROS, acetylcholinesterase (AChE) activity, and amyloid β peptides plaques in the brain. The limitations and side effects of existing synthetic drugs incline toward natural sources. In the present communication active principles of methanolic extract of Olea dioica Roxb, leaves are explored as an antioxidant, AChE inhibitor, and anti-amyloidogenic. Furthermore, neuroprotection against the amyloid beta-peptide has been studied. The bioactive principles were identified by GC-MS and LC-MS and further subjected to antioxidant (DPPH and FRAP) and neuroprotection (AChE inhibition, ThT binding, and MTT assay, DCFH-DA and lipid peroxidation (LPO) assay using neuroblastoma (SHSY-5Y) cell lines) assays. Methanolic extract of O. dioica Roxb, leaves was found to contain polyphenols and flavonoids. In vitro assays exhibited potential antioxidant and anti-AChE (˃50%) activities. ThT binding assay indicated protection against amyloid-beta aggregation. MTT assay, Aβ1-40 (10 µM) with extract increase the cell viability (˃50%) and showed significant cytotoxicity to SHSY-5Y cells. ROS level (˃25%) significantly decreased in the Aβ1-40 (10 µM) + extract (15 and 20 μM/mL) and LPO assay (˃50%) suggesting prevention of cell damage. Results advocate that O. dioica leaves are a good source of antioxidants, anti-AChE, and anti-amyloidogenic compounds which may be further evaluated as a natural medicine for the treatment of AD.

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Exploration of bioactive compounds from Olea dioica in Western Ghats of Karnataka using GC–MS

Krishnappa,

Karthik,

Pratap

et al. 2024

3 Biotech

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Anticholinesterase Activity and Mass Spectral Analysis of Olea dioica Roxb., An in vitro Study

Cited by 5 publications

References 33 publications

Augmentation of in vitro cytotoxic potentiality of crude methanolic leaf extract of Olea dioica as a pivotal anticancer source

Augmentation of in vitro cytotoxic potentiality of crude methanolic leaf extract of Olea dioica as a pivotal anticancer source

In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Exploration of bioactive compounds from Olea dioica in Western Ghats of Karnataka using GC–MS

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