Anticoagulant and antioxidant activity of sulfated chitosan from the shell of donacid clam Donax scortum (Linnaeus, 1758)

Abstract

Objectives: Sulfated chitosan was prepared from the shell of donacid clam Donax scortum by following demineralization, deproteinization, deacetylation and sulfating with chlorosulfonic acid. Results: FT-IR spectrum proved the structure of sulfated chitosan. The yield, sulfate content and molecular weight was found 80.30%, 14.2% and 18.65 × 10 4 Da. Their anticoagulant activity was determined for human plasma with respect to the activated partial thromboplastin time (APTT) and prothrombin time (PT) and it was 6.45 and 1.73 IU, respectively. The antioxidant activity of sulfated chitosan was evaluated as radical scavengers against superoxide anion (71.46% at 0.5 mg/ ml) and hydroxyl radicals (64.73% at 3.2 mg/ml). The antioxidant activity of sulfated chitosan was evaluated as radical scavengers against superoxide anion and hydroxyl radicals of 71.46% at 0.5 mg/ml and 64.73% at 3.2 mg/ml. The chelating ability and reducing power of sulfated chitosan was 43.24 at 10 mg/ml and 0.578 at 0.75 mg/ml. IC 50 values of scavenging abilities on superoxide anion and hydroxyl radicals were 0.398 and 0.527 mg/ml whereas those of chelating ability and reducing power were 5.01 and 0.728 mg/ml. Conclusion: These results indicated that sulfated chitosan from D. scortum had potent anticoagulant and antioxidant activities.

Evaluation of antibacterial, teratogenicity and antibiofilm effect of sulfated chitosans extracted from marine waste against microorganism

“…The mineral composition was maximum in sulfated chitosan of crab; expect CP, K, and ZN. The difference in the minerals contents were due to the source of the species, the present result was associated with the result of Subhapradha et al, 19 who stated that the crab shells as whole forms exoskeleton gives higher yield and mineral contents shown in previous studies.…”

“…The physicochemical, rheological, and biological properties of polysaccharides differ notably as a utility of its molecular mass and molecular weight distribution. Subhapradha et al 19 reported, sulfated chitosan isolated from D. scortum showed a molecular weight of 18.65 × 10 4 Da and it was readily soluble in water. Broadly, the influence of molecular weight plays a key role in the biological activity of sulfated polysaccharides.…”

The evaluation of antioxidant and antifungal properties of 6-amino-6-deoxychitosan in vitro

“…Chelating agents may serve as the secondary antioxidants because they reduce the redox potential thereby stabilizing the oxidized form of metal ions [37]. In Fig.…”

A glimpse on the function of chitosan as a dental hemostatic agent

“…Crustaceans such as crabs, lobsters, prawns, shrimps, and insects have outer shells/exoskeletons which are primary sources of chitin [ 2 , 4 ]. Chitin is soluble in many inorganic and organic acids, such as acetic, lactic, malic, formic, and succinic acids, but is insoluble in most organic solvents [ 5 ] and aqueous solutions of pH > 7. Chitosan is polycationic at pH < 6 due to the protonation of its amino group and interacts readily with anionic polysaccharides, forming polyelectrolyte complexes [ 2 , 6 ].…”