Anticoccidial Activity of Berberine against Eimeria-Infected Chickens

Nguyen, Binh Thanh; Flores, Rochelle A.; Cammayo, Paula Leona T.; Kim, Suk; Kim, Woo Hyun; Min, Wongi

doi:10.3347/kjp.2021.59.4.403

Cited by 11 publications

(8 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The other species such as E. maxima, E. necatrix, and E. mitis had a prevalence of 31.3% [20]. There have also been studies of the use of various natural substances to control coccidiosis, such as aloe vera [13], berberine [21], galla rhois [22] and green tea [23]. The objective of this study is to provide an up-to-date status of coccidiosis in Korea by investigating the occurrence of Eimeria infection in poultry farms and comparing the resistance of field Eimeria species to commercially available anticoccidial drugs.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological investigation and drug resistance of Eimeria species in Korean chicken farms

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Coccidiosis is a poultry disease that occurs worldwide and is caused by Eimeria species. The infection is associated with reduced feed efficiency, body weight gain, and egg production. This study aimed to investigate the current status of coccidiosis and anticoccidial resistance to anticoccidial drugs used as part of control strategies for this disease in Korean chicken farms. Results An overall prevalence of 75% (291/388) was found. Positive farms contained several Eimeria species (mean = 4.2). Of the positive samples, E. acervulina (98.6%), E. maxima (84.8%), and E. tenella (82.8%) were the most prevalent species. Compared with cage-fed chickens, broilers and native chickens reared in free-range management were more at risk of acquiring an Eimeria infection. Sensitivities to six anticoccidial drugs (clopidol, diclazuril, maduramycin, monensin, salinomycin, and toltrazuril) were tested using nine field samples. Compared with untreated healthy control chickens, the body weight gains of infected chickens and treated/infected chickens were significantly reduced in all groups. Fecal oocyst shedding was significantly reduced in four clopidol-treated/infected groups, three diclazuril-treated/infected groups, two toltrazuril-treated/infected groups, one monensin-treated/infected group, and one salinomycin-treated/infected group, compared with the respective untreated/infected control groups. Intestinal lesion scores were also reduced in three clopidol-treated/infected groups, one monensin-treated/infected group, and one toltrazuril-treated/infected group. However, an overall assessment using the anticoccidial index, percent optimum anticoccidial activity, relative oocyst production, and reduced lesion score index found that all field samples had strong resistance to all tested anticoccidial drugs. Conclusion The results of this large-scale epidemiological investigation and anticoccidial sensitivity testing showed a high prevalence of coccidiosis and the presence of severe drug resistant Eimeria species in the field. These findings will be useful for optimizing the control of coccidiosis in the poultry industry.

show abstract

Section: Introductionmentioning

confidence: 99%

Epidemiological investigation and drug resistance of Eimeria species in Korean chicken farms

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…is a highly crucial disease and main threats for the health and wellness of poultry [43][44][45]. E. tenella is dangerous because of its pervasiveness and capability of causing infection [5].…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics-based prediction of biochemical characteristic and potential immunogenic epitopes for ROPs proteins of the E. tenella as candidate vaccine target

Mohammadi,

Ghaffari

2024

Preprint

View full text Add to dashboard Cite

Introduction: Coccidiosis is an essential type of disease which is responsible for infection all around the world. The mentioned disease is caused by Eimeria which has determinable characteristics including intestinal injury, diarrhea, or bloody stools. Despite the using specific drugs for this disease, a lot of issues like the onset of drug-resistance and raised treatment costs made the drug mediated method challenging. The present in silico investigation was done to reveal biophysical properties and immunogenic epitopes of six ROPs proteins for rational vaccine design in future. Materials and methods: We utilized different online tools in order to predict characteristics like allergenicity, antigenicity, physicochemical properties, solubility, post-translational modification sites (PTMs), the presence of signal peptide and transmembrane domains. In addition, the secondary and tertiary structures of the assessed proteins were drawn through the refinement process and validation. At last, we utilized NetCTL server as a prediction tool for cytotoxic T lymphocyte (CTL) epitopes. After the mentioned process, immunogenicity was analyzed. IEDB server was chosen as a prediction tool for helper T-lymphocyte (HTL) epitopes. After the mentioned process, we assessed antigenicity and IFN-γ and IL-4 induction. Results: We could find many different linear B-cell epitopes with ability to be antigenic. They also had good water solubility, and they didn't have allergenicity. Thus, these proteins had proper antigenicity, and they had many different PTMs. They also had different HTL, CTL, and B-cell epitopes. We can use these results to develop a proper kind of multi-epitope vaccine for therapeutic purposes. Conclusion: According to the present study, it might be promising to focus on specific regions of the parasite’s protein rather than large protein residues, as this might contribute to the reduction of the parasite’s antigen complexity.

show abstract

“…In recent years, a variety of Chinese herbal extracts, such as Shi Ying Zi [ 27 ], berberine [ 28 ], the mushrooms Agaricus subrufescens and Pleurotus ostreatus [ 29 ], Cinnamomum verum [ 30 ] and Canary rue [ 31 ], have been reported to show anticoccidial effects to some extent. EA, a polyphenol extracted from certain medicinal plants, is a member of the tannin family that possesses several biological properties, including antioxidant and anti-inflammatory properties [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Oral administration of Lactobacillus brevis 23017 combined with ellagic acid attenuates intestinal inflammatory injury caused by Eimeria infection by activating the Nrf2/HO-1 antioxidant pathway

Yang

Pan

Jia

et al. 2022

Vet Res

View full text Add to dashboard Cite

The aim of this study was to investigate whether oral administration of Lactobacillus brevis 23017 (LB) alone and in combination with ellagic acid inhibits ChTLR15/ChNLRP3/ChIL-1β by activating the Nrf2/HO-1 pathway to attenuate intestinal inflammatory injury. Two animal experiments were performed. In Experiment 1, chickens were allocated into 7 groups: PBS, and low, medium and high dosages of live and heat-killed LB, named L/LB(+), M/LB(+) and H/LB(+), and L/LB(−), M/LB(−) and H/LB(−), respectively. In Experiment 2, chickens were divided into 5 groups: PBS, challenge control, and low, medium and high dosages of ellagic acid combined with LB(+), named L/EA + L/LB(+), M/EA + M/LB(+) and H/EA + H/LB(+), respectively. Chickens were gavaged with LB with or without ellagic acid once a day. Then, the mRNA and protein levels of the components of the Nrf2/HO-1 pathway found in the caecal tissues were quantified. On Day 7 post-infection with E. tenella, the levels of the components of the ChTLR15/NLRP3/IL-1β pathway in the caeca were again quantified, and the anticoccidial effects were assessed. The results showed that the levels of the genes in the Nrf2/HO-1 pathway in the chickens in the LB(+) groups were higher than those in the LB(−) groups (p < 0.001); those in the H/LB(+) group were higher than those in the M/LB(+) and L/LB(+) groups (p < 0.001); and those in the H/EA + H/LB(+) group showed the highest expression levels compared with the other groups (p < 0.001). After challenge, the chickens in the H/LB(+) group displayed less inflammatory injury than those in the M/LB(+) and L/LB(+) groups (p < 0.05), and the chickens in the H/EA + H/LB(+) group showed stronger anti-inflammatory effects than the other groups (p < 0.05). Thus, these protective effects against infection were consistent with the above results. Overall, significant anti-inflammatory effects were observed in chickens orally gavaged with high dosages of live L. brevis 23017 and ellagic acid, which occurred by regulation of the ChTLR15/NLRP3/IL-1β pathway.

show abstract

Anticoccidial Activity of Berberine against Eimeria-Infected Chickens

Cited by 11 publications

References 32 publications

Epidemiological investigation and drug resistance of Eimeria species in Korean chicken farms

Epidemiological investigation and drug resistance of Eimeria species in Korean chicken farms

Bioinformatics-based prediction of biochemical characteristic and potential immunogenic epitopes for ROPs proteins of the E. tenella as candidate vaccine target

Oral administration of Lactobacillus brevis 23017 combined with ellagic acid attenuates intestinal inflammatory injury caused by Eimeria infection by activating the Nrf2/HO-1 antioxidant pathway

Contact Info

Product

Resources

About