Antidermatophyte and antimelanogenesis compound from Eleutherine americana grown in Indonesia

Abstract

An active compound from the bulb of Eleutherine americana L. Merr. (Iridaceae) collected from East Kalimantan, Indonesia, was tested for its antidermatophyte and antimelanogenesis activity. Antifungal assay-directed fractionation of the n-hexane-soluble fraction of the methanolic extract of the bulb of E. americana led to the isolation of 1 as an active compound. The compound was identified as the naphthoquinone eleutherin by EI-MS and (1)H-, (13)C-, and two-dimensional NMR analyses. Antidermatophyte assay of 1 at concentrations of 10, 20, 40, 60, and 80 microg/disk and myconazole, a commercial antidermatophyte, at 10 microg/disk displayed 7, 8, 13, 16, 17, and 14 mm of inhibition zone against Trichophyton mentagrophytes, respectively. In a melanin formation inhibition assay, compound 1 displayed potent antimelanogenesis activity at 5 ppm with low toxicity compared with arbutin, a commercial skin-whitening agent. The results showed the high potential of 1, an active compound from E. americana, to be applied as an antidermatophyte and antimelanogenesis agent.

Biological Activity and Phytochemical Analysis of Three Indonesian Medicinal Plants, Murraya koenigii, Syzygium polyanthum and Zingiber purpurea

“…Plant extracts were dissolved in acetone to obtain a concentration of 40, 60, and 80 μg/10 μL, which was selected on the basis of our preliminary results showing the antimicrobial activity of plant extract tested simultaneously to correlate with the concentration of standard drugs used (10−20 μg/disk). Antimicrobial assays were conducted using the disc diffusion method as previously described by Kusuma et al [6]. Zones of inhibition around the discs were measured in mm.…”

Enhanced cytotoxic potential of Orthosiphon stamineus extract in MCF-7 cells through suppression of nucleolin and bcl2

“…Eleutherol is a napthofuran, one of its derivatives eleutherine showed interesting inhibitory activity against human topoisomerase II, and compounds with this action can be considered as part of first-line chemotherapy for a wide variety of tumors (Kusuma et al, 2010).…”

Antimicrobial Properties of Leptadenia Hastata (Pers) Decne Leaves Extract

“…The development of microbial resistance to presently available antibiotics has led to the search for new antimicrobial agents [16]. Due to the problem of microbial resistance to antibiotics, attention is given toward biologically active components isolated from plant species commonly used as herbal medicine, as they may offer a new source of antimicrobial activities [17].…”

“…A major focus of natural product in chemistry has been toward drug design and discovery. However, obtaining scientific proof for the biological activity of natural plants is still challenging [5,6].…”