Enhanced cytotoxic potential of Orthosiphon stamineus extract in MCF-7 cells through suppression of nucleolin and bcl2

Saravanan, Renuka; Brindha, P.; Sridharan, Sriram; Narayanan, Mahesh; Ramalingam, Sivakumar

doi:10.3329/bjp.v12i3.32337

Cited by 6 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar result was observed for several human colon cancer cell lines treated with Coleus amboinicus leaves (WiDr cells) [ 167 ] and Vitex rotundifolia fruit (HCT-116, SW480, LoVo and HT-29 cells) extracts [ 146 ]. In addition, apoptotic signals have been triggered in breast cancer cell lines after incubation with extracts of Plectranthus amboinicus leaves (MCF-7 cells) [ 120 ], Orthosiphon stamineus leaves (MCF-7 cells) [ 168 ], Melissa officinalis leaves (MCF-7 cells) [ 166 ], Vitex rotundifolia leaves (T-47D cells) [ 159 ], and Prunella vulgaris root (MCF-5 cells) [ 150 ]. Changes in the expression level of Bcl-2 family protein are also induced in prostate cancer cell lines by extracts of Ocimum sanctum leaves (LNCaP cells) [ 160 ], Dracocephalum palmatum (PC-3 cells) [ 169 ], and Melissa officinalis leaves (PC-3) [ 166 ].…”

Section: The Lamiaceae Family Plantsmentioning

confidence: 99%

“…The insertion of Bax/Bak into the mitochondrial membrane results in the formation of a pore complex and release of cytochrome c into the cytosol from the intermembrane space. In contrast, Bcl-2 and Bcl-xl prevent the release of cytochrome c. Excessive levels of cytochrome c were observed in MCF-7 cells after Orthosiphon stamineus leaf extract treatment [ 168 ]. Cytochrome c binds to apoptotic protease-activating factor 1 (Apaf-1) to create an apoptosome that activates caspase-9.…”

Section: The Lamiaceae Family Plantsmentioning

confidence: 99%

“…The Fas ligand–receptor junction is inhibited by nucleolin, which is a protein that protects against apoptotic death [ 175 ]. Orthosiphon stamineus leaf extract treatment significantly decreased the nucleolin level in MCF-7 cells [ 168 ]. The attachment of a Fas to a specific receptor triggers the formation of specific death-inducing signaling complex (DISC) possessing a Fas-associated death domain (FADD); this complex can recruit an adaptor protein and then activate initiator caspase-8 and caspase-10.…”

Section: The Lamiaceae Family Plantsmentioning

confidence: 99%

See 2 more Smart Citations

An In Vitro Evaluation of the Molecular Mechanisms of Action of Medical Plants from the Lamiaceae Family as Effective Sources of Active Compounds against Human Cancer Cell Lines

Sitarek

Merecz-Sadowska

Śliwiński

et al. 2020

Cancers

View full text Add to dashboard Cite

It is predicted that 1.8 million new cancer cases will be diagnosed worldwide in 2020; of these, the incidence of lung, colon, breast, and prostate cancers will be 22%, 9%, 7%, and 5%, respectively according to the National Cancer Institute. As the global medical cost of cancer in 2020 will exceed about $150 billion, new approaches and novel alternative chemoprevention molecules are needed. Research indicates that the plants of the Lamiaceae family may offer such potential. The present study reviews selected species from the Lamiaceae and their active compounds that may have the potential to inhibit the growth of lung, breast, prostate, and colon cancer cells; it examines the effects of whole extracts, individual compounds, and essential oils, and it discusses their underlying molecular mechanisms of action. The studied members of the Lamiaceae are sources of crucial phytochemicals that may be important modulators of cancer-related molecular targets and can be used as effective factors to support anti-tumor treatment.

show abstract

Section: The Lamiaceae Family Plantsmentioning

confidence: 99%

Section: The Lamiaceae Family Plantsmentioning

confidence: 99%

Section: The Lamiaceae Family Plantsmentioning

confidence: 99%

See 1 more Smart Citation

An In Vitro Evaluation of the Molecular Mechanisms of Action of Medical Plants from the Lamiaceae Family as Effective Sources of Active Compounds against Human Cancer Cell Lines

Sitarek

Merecz-Sadowska

Śliwiński

et al. 2020

Cancers

View full text Add to dashboard Cite

show abstract

“…The cytotoxic effect of Orthosiphon stamineus extract in MCF-7 cells is due to suppression of nucleolin and Bcl2 (Saravanan et al, 2017).…”

Section: Author Infomentioning

confidence: 99%

Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling

Chen

2018

Bangladesh J Pharmacol

View full text Add to dashboard Cite

Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling BJP IntroductionBreast cancer is the second leading cause of cancer death among women after lung cancer in the United States (DeSantis et al., 2014). In the treatment for breast cancer, some adverse effects could cause serious complications and affect the health and quality of life among survivors (Odle, 2014). Therefore, it is necessary to develop novel anti-cancer candidates for breast cancer.In the past several decades, natural compounds such as resveratrol (Sinha et al., 2016), epigallocatechin-3-gallate, oldenlandia diffusa and Ziziphus jujube have been demonstrated that possessed potential anti-breast cancer activity (Bonofiglio et al., 2016).Lycorine, known as a multifunctional compound, is extensively studied for its pharmacological activities, such as anti-tumor activity (Lamoral-Theys et al., 2010). Previous studies have shown that lycorine presented a potential anti-cancer activity in breast cancer via the inhibition of tumor growth and metastasis Ying et al., 2017). Src/FAK (focal adhesion kinase) -involved pathway and STAT3 (signal transducer and activator of transcription 3) signaling pathway were involved in growth, invasion and metastasis of breast cancer.However, Wnt/ -catenin signaling, a multifunctional pathway that has not been studied to be involved in proliferation, invasion and migration of breast cancer. This study is meant to confirm the inhibitory effects of lycorine on the proliferation, migration and invasion of MDA-MB-231 breast cancer cells and investigate the activation of Wnt/ -catenin signaling pathway involved. Materials and Methods Cell lines and reagentsHuman MDA-MB-231 breast cancer cells were obtained AbstractThis study was aimed to determine the effects of lycorine, a toxic crystalline alkaloid, on MDA-MB-231 breast cancer cells proliferation, migration and invasion, and to investigate the mechanism involved. The cells were cultured with different concentrations of lycorine in vitro. MTT assays were performed to determine the proliferation of cells. Transwell assays were performed to measure the migration and invasion of cells. The activation of Wnt/ -catenin signaling pathway and expression were assayed by Western blot. This study showed that proliferation, migration and invasion of MDA-MB-231 breast cancer cells could be inhibited by lycorine. Furthermore, we found that Wnt/ -catenin signaling was markedly blocked in MDA-MB-321 cells treated with lycorine. In conclusion, lycorine inhibits the proliferation, migration and invasion of MDA-MB-231 breast cancer cells that is associated with the suppression of Wnt/ -catenin signaling. Article Info

show abstract

Clerodendranthus Spicatus: A review of its active compounds, mechanisms of action, and clinical studies in urinary diseases

Li,

Raza,

Jiang

et al. 2024

Fitoterapia

View full text Add to dashboard Cite

Enhanced cytotoxic potential of Orthosiphon stamineus extract in MCF-7 cells through suppression of nucleolin and bcl2

Cited by 6 publications

References 19 publications

An In Vitro Evaluation of the Molecular Mechanisms of Action of Medical Plants from the Lamiaceae Family as Effective Sources of Active Compounds against Human Cancer Cell Lines

An In Vitro Evaluation of the Molecular Mechanisms of Action of Medical Plants from the Lamiaceae Family as Effective Sources of Active Compounds against Human Cancer Cell Lines

Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling

Clerodendranthus Spicatus: A review of its active compounds, mechanisms of action, and clinical studies in urinary diseases

Contact Info

Product

Resources

About