ANTIDERMATOPHYTIC ACTIVITY OF GREEN SYNTHESISED ZINC OXIDE NANOPARTICLES USING Cassia alata LEAVES

Sujatha, J.; Asokan, S.; Rajeshkumar, S.

doi:10.15414/jmbfs.2018.7.4.348-352

Cited by 24 publications

(16 citation statements)

References 14 publications

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“…This image confirmed the value of XRD crystallite data. Compared to our study, the morphology of the biosynthesized ZnO-NPs was presented by Sujatha et al, with similar shapes and sizes ranging between 80 and 130 nm [ 43 ].…”

Section: Resultsmentioning

confidence: 44%

Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells

Aalami

Mesgari

Sahebkar

2020

Bioinorganic Chemistry and Applications

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Changes in the expression of microRNAs can affect cancer cells’ viability and behavior and the impact on cancer treatment. In this study, the expression of miR-155-5p, miR-203a-3p, and miR-223-3p in the MCF7 cancer cell line was studied when exposed to ZnO nanoparticles synthesized through a green route. Mentioned ZnO-NPs were well characterized by UV-vis spectroscopy, DLS, XRD, FTIR, FE-SEM, EDX, zeta potential, and AFM analyses. Cellular studies were conducted using ZnO-NPs before miRNA investigations including MTT cytotoxicity test against MCF7, MDA-MB-231, and HFF cell lines. Moreover, apoptosis assays were performed using morphological analysis, fluorescent dyes, flow cytometry, and evaluation of caspase-3 and caspase-8 gene expression. Biological properties such as the antioxidant and antimicrobial activity of these novel ZnO-NPs were considered. MTT assays showed that the inhibitory concentration (IC50) of ZnO-NPs after 24 h was 11.16 μg/mL, 60.08 μg/mL, and 26.3 μg/mL on MCF7, MDA-MB-231, and HFF cells, respectively. The qRT-PCR results showed reduced expression of miR-155-5p, miR-203a-3p, and miR-223-3p when the MCF7 cells were treated with the IC50 concentration of ZnO-NPs (11.16 μg/mL). The antioxidant activity results showed EC50 values at 57.19 μg/mL and 31.5 μg/mL in DPPH and ABTS assays, respectively. The antimicrobial activity of ZnO-NPs was determined on Gram-negative and Gram-positive bacterial strains and fungi using MIC and MBC assays. These NPs had a significant effect in reducing the expression of microRNAs in breast cancer cells. Finally, ZnO-NPs exerted antioxidant and antimicrobial activities.

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Section: Resultsmentioning

confidence: 44%

Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells

Aalami

Mesgari

Sahebkar

2020

Bioinorganic Chemistry and Applications

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“…Therefore, the present finding clearly suggests the potential application of Brassica oleracea extract prepared ZnO nanocrystals as an excellent antibacterial agent. The zinc oxide nanoparticles are actively involved in the antibacterial activity and may used for different types of biomedical applications based on this property [10,[22][23][24].…”

Section: Resultsmentioning

confidence: 99%

Brassica oleracea Mediated Synthesis of Zinc Oxide Nanoparticles and its Antibacterial Activity against Pathogenic Bacteria

Rajeshkumar¹,

Agarwal²,

Kumar³

et al. 2018

Asian J. Chem.

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Nano-sized materials are known to play important role in basic and applied sciences thus the metal and metal oxide nanoparticles have been intensively studied in the past decade [1,2]. The metal oxides particularly exhibit fascinating properties such as electronic, catalytic, magnetic and antimicrobial activity owing to their large surface area to volume ration due to a high fraction of atoms [3,4]. Zinc oxide nanoparticles have a wide band gap of 3.3 eV at room temperature and high excitation binding energy of 60 meV, which helps it to demonstrate outstanding catalytic, optical, photochemical and electronic properties [5,6]. Zinc oxide nanoparticles are known to possess unique UV filtering, antibacterial, antifungal properties also which leads to its extensive use in cosmetic and healthcare industries [7-9]. Previous studies suggest that the strong antibacterial activity of zinc oxide nanoparticles against a variety of pathogenic Gram-positive and Gram-negative bacteria attributed to its capacity of releasing Zn 2+ ion from ZnO complex and interruption of lipid bilayer integrity, ROS generation and subsequent damage to DNA and protein of the host cell [10,11]. Chemical and physical methods of nanoparticle synthesis have

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“…26 Type I diabetes incidence was increasing during the past several decades in all the developed countries around the world, suggesting that these changes in the environment may influence disease pathogenesis due to the alterations in the natural flora of gut microbiota. 27…”

Section: Type I Diabetesmentioning

confidence: 99%

A Current Review on Gut Microbiota For Healthy Life

2020

IJFMT

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The human gastrointestinal tract is highly populated by a vast and diverse community of different microbes. Every individual is provided with a unique gut microbiota which varies according to their food habits and dietary intakes. This human gastrointestinal tract gut microbiota has various functions by participating in host mechanisms or metabolisms acts as or gives protection against the pathogens invading microbes into the gut,and also play an important role in facilitating the immune system development and function. An slight alteration even in this natural flora of gut in the intestine can cause infectious disease and disorders to the human. Various infections due to antibiotics and gut bacterial communities,bacterial interactions,mood disorders,metabolic disorders,diabetes,allergic disease,inflammatory bowel disease,hormonal and many neurological processes can happen. Hence, it is very important in maintaining the natural flora of the gut as it maintains a healthy gut microbiota , structural integrity and protection. Recently ayurvedic medicines are used over allopathy for its less sides on the body and for maintaining a healthy gut microbiome. This review aims on the current status of the gut microbiota for healthy life under key problems identification and maintaining the current healthy gut microbiota in the human gastrointestinal tract.

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ANTIDERMATOPHYTIC ACTIVITY OF GREEN SYNTHESISED ZINC OXIDE NANOPARTICLES USING Cassia alata LEAVES

Cited by 24 publications

References 14 publications

Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells

Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells

Brassica oleracea Mediated Synthesis of Zinc Oxide Nanoparticles and its Antibacterial Activity against Pathogenic Bacteria

A Current Review on Gut Microbiota For Healthy Life

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