The green techniques are plying a major role in the synthesis of nanoparticles and in this present study we used the herbal plant Cassia alata for the green synthesis of zinc oxide nanoparticles. This reducing agent generates zinc oxide nanoparticles (ZnO NPs) from zinc acetate solution within 15 minutes. The synthesized ZnO NPs was characterized by UV-vis spectrophotometer shows surface Plasmon resonance band at 335 nm. Scanning electron microscope (SEM) image showed the size of the crystalline nanoparticles ranging from 30- 50 nm. Energy dispersive X-ray analysis (EDAX) confirms the presence of elemental Zn and O in the synthesized nanoparticles. FTIR revealed that presence of functional molecules like carboxylic acids, amine ant nitro groups associated with synthesized oxide nanoparticles. Atomic force microscope (AFM) shows spherical shape of nanoparticles as well as aggregation also observed. Thus green synthesized nanoparticles were tested for their antidermatophytic potential against Trichophyton mentagrophyte, Trichophyton rubrum, Epidermophyton floccosum, Microsporum audouinii, and Microsporum canis and recorded percentage of inhibition, MIC and MFC values.

The aim of the present investigation is to screening of phytochemical constituents for its free radical scavenging activity of ethanol extract of Cassia alata leaves. The preliminary screening was carried out by standard chemical method. It evidenced that the presence of phytochemicals such as alkaloids, carbohydrate, phenols, tannins, falvanoids, steroids, protein and sugar in the ethanol extract. Various functional groups like alcohols, phenols, carboxylic acid and nitro groups were associated with the extract were characterized using Fourier transform infrared spectroscopy (FTIR). A few common compounds available in the extract were identified by Gas chromatography -Mass spectrum (GC-MS) analysis. The DPPH free radical scavenging activity and 50 % inhibitory concentration (IC 50 ) was calculated and compared with the standard synthetic drug BHT. The IC 50 value of leaf extract was found to be 24.56 µg/ml respectively. This study revealed that C. alata ethanol extract of leaves has alternative drugs for free radical it could be acted as a novel source of free radical scavengers.

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Cassia alata is used traditionally to cure various diseases like ulcers, stomach pain and fever. The potential of antidermatophytic, free radical scavenging and cytotoxic activity of ethanol leaves extracts of C. alata was studied. The preliminary phytochemical screening assay elucidates that presence of carbohydrates, tannin, phenol, saponin, flavonoid, steroid, terpenoids, glycosides, and alkaloids. Further phytochemical extraction was done by GC-MS, HPLC and FTIR. GC-MS analysis confirms that the presence of two major compounds namely Hexadecanoic acid and Oleic acid with four minor compounds. HPLC techniques revealed that the presence of two major compounds observed in two different retention time. FTIR spectrum revealed that the presence of carboxylic groups, alkanes, aldehydes and aliphatic amines in the ethanol extract of C. alata leaves. Cytotoxic effect of ethanol extract was assayed at different concentrations like 10, 25, 50 and 100 μg/ml against human epithelial carcinoma (A431). At100 μg/ml of ethanol concentration, 93.26±5.44 (%) and 82.45±2.44 (%) percentage of cancer cells were inhibited by ethanol extract and standard drug, respectively. Microscopic images of cancer cells treated with ethanol extract confirm the morphological changes and shrinkages of cells leads to cell death. These results were suggesting that Cassia alata exhibit high potential antioxidant, antidermatophytic and cytotoxic activity and also it could serve as alternative new resources for manufacturing new drugs against newly evaluating diseases.