Studies on the antioxidant activity of ethanol extract of Cassis alata using FT-IR, HPLC and GC-MS analysis

Sujatha, J.; Asokan, S.

doi:10.22192/ijarbs.2017.04.12.012

Cited by 9 publications

(9 citation statements)

References 14 publications

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“…In contrast, the tannins, steroids / terpenoids, and Reducing compounds were not detected. This result is contrary to a team of researchers who indicated the presence of alkaloids, phenols, tannins, flavonoids, saponins, steroids and sugar in the aqueous extract of the leaves of S. alata 26 . The qualitative chemicals are an indication of an antioxidant and anti-inflammatory values of this plant and may be responsible for the traditional uses of S. alata leaves.…”

Section: Discussioncontrasting

confidence: 99%

In Vitro Antioxidant Property and Phytochemical Constituents of Senna alata Leaves Aqueous Extract Collected in Ngaoundéré (Cameroon)

Vedekoi

Selestin

2020

PBJ

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Leaves extract of Senna alata L. is a plant used as indigenous medicine to treat various types of disease like ulcers, stomach, pain and fever. The present study was undertaken to study the phytochemical screening, total phenolic compounds (TPC), total flavonoid compounds (TFC) and in vitro antioxidant of Senna alata leaves’ extract growing in Ngaoundéré town (Cameroon). Respective bioactivities of the phytochemicals were determined. Quantitative analysis of the total phenolic content was determined by using the Folin-Ciocalteu method while total flavonoid was estimated using aluminium trichloride (AlCl3). The antioxidant capacities in the forms of DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) were evaluated by spectrophotometric methods. For these purposes, aqueous extract were prepared. Now the experimental screening of phytochemicals showed negative results for the absence of reducing compounds, steroids/triterpenes, and tannins. The results showed that TPC, TFC values were higher: 14.768±0.26 mg GAE per g of extract, 4.32±0.12 mg Ru per g of extract, respectively. The Senna alata Leaves’ extract (SALE) exhibited the best DPPH inhibition concentration 50% (IC50 = 12.05 mg. mL-1), and FRAP method (IC50 = 2.79 mg. mL-1) compared to that of the positive control, ascorbic acid (IC50 = 17.69 mg/mL); Hence, extract from the leaves of Senna alata contains high secondary metabolites which accounts for its strong antioxidant ability thus justifying its use as natural occurring antioxidants in folkloric medicine.

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Section: Discussioncontrasting

confidence: 99%

In Vitro Antioxidant Property and Phytochemical Constituents of Senna alata Leaves Aqueous Extract Collected in Ngaoundéré (Cameroon)

Vedekoi

Selestin

2020

PBJ

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“…Previous studies on Cassia alata chemical composition have also shown a wide range of phenolic compounds including kaempferol, rhein, chrysophanol, aloe-emodin and physcion ( Fernand et al, 2008 ). It has demonstrated considerable free radical scavenging effects ( Sujatha & Asokan, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antioxidant and neuroprotective activities of Cassia fistula (L.) using the Caenorhabditis elegans model

Thabit

Handoussa

Roxo

et al. 2018

PeerJ

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BackgroundCassia fistula (L.) (Fabaceae) is a medicinal plant from tropical Asia. It is known for its marked antioxidant activity, which is attributed to its high phenolic content. The present study aims at testing both the antioxidant and neuroprotective effects of a hydroalcoholic extract from the aerial parts of Cassia fistula using the Caenorhabditis elegans model, which is widely used in this context.MethodsChemical profiling of secondary metabolites that seem to be responsible for both antioxidant and neuroprotective capacities was carried out by HPLC/PDA/ESI-MSn. Antioxidant activity was tested in vitro by CUPRAC and DPPH assays. In vivo antioxidant and neuroprotective activities were investigated using the C. elegans model.ResultsThe Cassia extract improved the survival rate of the nematodes and protected them against oxidative stress. In addition, a decrease in the accumulation of reactive oxygen species (ROS) was observed. The important role of DAF-16/FOXO pathway was confirmed through an increased nuclear localization of the DAF-16 transcription factor, increased expression of SOD-3 stress response gene and decreased expression of HSP-16.2. Furthermore, the putative involvement of SKN-1/NRF2 pathway was demonstrated by a decrease in GST-4 levels. A neuroprotective activity of the Cassia extract was shown by a decline in polyglutamine (polyQ40) aggregate formation and a delay in paralysis caused by amyloid beta (Aβ1–42) accumulation.DiscussionThe Cassia extract exhibits substantial antioxidant and neuroprotective activities in vivo, which might provide a rich and novel source of natural antioxidants and neuroprotective compounds to be further studied for the use in various food and cosmetic industrial fields.

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“…Octadecanoic acid have been reported as anticarcinogen or antitumoral (Hsouna et al, 2011;Gehan et al, 2009). Fatty acid like butyl hexadecanoate, methyl hexadecanoate, and methyl octadecanoate, has antimicrobial activity (Sujatha et al, 2014;Abubakar & Majinda, 2016). Benzenoids like 2-(2-ethylhexoxycarbonyl)benzoic acid ester has been reported as cytotoxic (Krishnan, Mani & Jasmine, 2014).…”

Section: Discussionmentioning

confidence: 99%

GC-MS analysis of phytochemical compounds of Opuntia megarrhiza (Cactaceae), an endangered plant of Mexico

Cupido

De‐Nova

Guerrero-González

et al. 2022

PeerJ Organic Chemistry

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Opuntia megarrhiza is an endemic plant used in Mexican traditional medicine for the treatment of bones fractures in humans and domestic animals. One of the most used technique for the detection and characterization of the structure of phytochemical compounds is the Gas Chromatography Coupled to Mass Spectrometry. The goals of the present study were to identify and characterize the phytochemical compounds present in wild individuals of O. megarrhiza using this analysis. We used chloroform and methanol extracts from cladodes, and they were analyzed by gas chromatography-electron impact-mass spectrometry. We obtained 53 phytochemical compounds, 19 have been previously identified with some biological activity. Most of these compounds are alkanes, alkenes, aromatic hydrocarbons, fatty acids, and ketones. We detected some fragmentation patterns that are described for the first time for this species. The variety of metabolites presents in O. megarrhiza justifies the medicinal use of this plant in traditional medicine and highlight it as a source of phytochemical compounds with potential in medicine and biotechnology.

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Studies on the antioxidant activity of ethanol extract of Cassis alata using FT-IR, HPLC and GC-MS analysis

Cited by 9 publications

References 14 publications

In Vitro Antioxidant Property and Phytochemical Constituents of Senna alata Leaves Aqueous Extract Collected in Ngaoundéré (Cameroon)

In Vitro Antioxidant Property and Phytochemical Constituents of Senna alata Leaves Aqueous Extract Collected in Ngaoundéré (Cameroon)

Evaluation of antioxidant and neuroprotective activities of Cassia fistula (L.) using the Caenorhabditis elegans model

GC-MS analysis of phytochemical compounds of Opuntia megarrhiza (Cactaceae), an endangered plant of Mexico

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