Antidiabetic activity of aqueous stem bark extract of Annickia polycarpa in alloxan-induced diabetic mice

Lartey, Nathaniel L.; Asare‐Anane, Henry; Ofori, Emmanuel Kwaku; Antwi, Stephen; Asiedu-Larbi, Jerry; Ayertey, Frederick; Okine, Laud Kenneth

doi:10.1016/j.jtcme.2020.02.001

Cited by 16 publications

(12 citation statements)

References 29 publications

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“… 2020 ; Lartey et al. 2021 ). Therefore, it is very important to find alternative therapies based on Chinese herbal medicine with fewer side effects and multiple targets.…”

Section: Discussionmentioning

confidence: 99%

Ranunculus ternatus Thunb extract attenuates renal fibrosis of diabetic nephropathy via inhibiting SMYD2

Peng

Chen

et al. 2022

Pharmaceutical Biology

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Context Ranunculus ternatus Thunb (Ranunculaceae), (RTT) is used clinically for the treatment of tuberculosis or as tumour adjuvant therapy, but its potential effect on diabetic nephropathy (DN) has not been studied. Objective To investigate the effect of RTT extract in renal fibrosis of DN. Materials and methods C57BL/6 mice were randomly divided into four groups ( n = 12). Diabetes mellitus (DM) mice were induced by streptozotocin (STZ, 55 mg/kg/day) for five consecutive days and treated by RTT extract (2 g/kg). Afterward, blood glucose, HE and Masson staining were assayed. The expression levels of Vimentin, ɑ-SMA, TNF-ɑ, NF-κB p-p65, NF-κB p65, SMYD2, H3K36me3, H3K4me3 were determined by western blots. Firbronectin was respectively assayed by western blot and immunofluorescent staining. Results RTT extract significantly ameliorated renal injury and renal fibrosis in the renal tissue of STZ-induced diabetic mice as demonstrated by the decreased expression level of Fibronectin (65%), Vimentin and α-SMA (75% & 53%). In addition, the levels of TNF-α (57%), NF-κB p-p65 and NF-κB p65 (35% & 25%) were elevated in the DN mice. Importantly, these were alleviated after RTT extract treatment. Moreover, we observed that the protein levels of SMYD2 (30%), H3K36me3 and H3K4me3 (53% & 75%) were reduced in DN mice after treatment with RTT extract. Discussion and conclusions RTT extract mediates antifibrotic effects and anti-inflammatory responses in STZ-induced DN mainly through suppressing SMYD2 activation and H3K36me3 and H3K4me3 protein expression. RTT extract might have therapeutic potential against high glucose-induced nephropathy.

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“… 2020 ; Lartey et al. 2021 ). Therefore, it is very important to find alternative therapies based on Chinese herbal medicine with fewer side effects and multiple targets.…”

Section: Discussionmentioning

confidence: 99%

Ranunculus ternatus Thunb extract attenuates renal fibrosis of diabetic nephropathy via inhibiting SMYD2

Peng

Chen

et al. 2022

Pharmaceutical Biology

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“…Diabetes mellitus is a metabolic disorder characterized by imbalance between carbohydrate, protein and lipid metabolism may be due to reduced insulin secretion or increased insulin resistance. 27 Higher blood glucose level in diabetes mellitus may result in secondary diabetes complications as a result of continuously elevated blood glucose level. The treatment approaches are mainly focused on maintaining normal blood glucose level to avoid probable secondary diabetic complications.…”

Section: Discussionmentioning

confidence: 99%

Effect Of Fruit And Cork Extract Of Ficus Lacor Buch Ham On α/β -Glucosidase, α -Amylase, Lipase, Glucose Absorption And Uptake

S¹,

Naikwade²

2022

Int J Life Sci Pharm Res

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Fruits of the plant Ficus Lacor Buch. Ham. were used traditionally for treatment of diabetes mellitus. The present study was undertaken to evaluate the antidiabetic potential of the plant using in vitro approach. Effect of Ficus Lacor Buch. Ham. was evaluated using α/β -glucosidase, α-amylase and lipase enzyme inhibition assay methods. The glucose absorption in intestine was evaluated using everted rat jejunum while glucose uptake was evaluated using isolated rat hemidiaphragm. Fruit and cork ethanolic extract was prepared by using soxhlation extraction method. In vitro assay of α-glucosidase showed that IC50 value of fruit extract was 83.03 µg/ml and cork extract 88.32 µg/ml when compared with control group acarbose. β-glucosidase enzyme was inhibited by fruit and cork extract of plant with IC50 value of fruit and cork extract 132.71 µg/ml and 171.93 µg/ml. The extracts further quantify α-amylase inhibitory activity of fruit (IC50 77.93 µg/ml) and cork (IC50 111.94 µg/ml) extract. Lipase inhibitory assay indicated the effect of plant extract on lipase enzyme was not prominent when compared to orlistat. Absorption of glucose through everted rat jejunum was reduced significantly (P ˂ 0.05) when compared with standard metformin. Effect of fruit and cork extract on rat hemidiaphragm exhibited significant (P ˂ 0.05) increase in glucose uptake when compared with standard metformin. Result suggests Ficus Lacor Buch. Ham. is effective in inhibiting carbohydrate metabolizing enzymes α/β –glucosidase and α-amylase while lipase enzyme was not affected. Fruit and cork extract of the plant was found to reduce significantly glucose absorption in everted rat jejunum. The significant increase in glucose uptake was observed in isolated rat diaphragm. The result reveals that Ficus Lacor Buch. Ham. acts by inhibiting carbohydrate metabolizing enzymes, reducing glucose absorption in intestine and increasing glucose uptake in hemidiaphragm

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“…Tail tipping method on Swiss-Albino mice [23][24][25] was utilized to measure the blood glucose level by cutting the tip of the tail through a sterile surgical blade, using ethyl chloride as a local anesthetic. The single-dose study was categorized into four groups consisting of four mice in each viz.…”

Section: Experimental Design and Categorizationmentioning

confidence: 99%

Does Rabeprazole Sodium Alleviate the Anti-diabetic Activity of Linagliptin? Drug-Drug Interaction Analysis by In vitro and In vivo Methods

et al. 2020

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Drug interaction has turned into the preeminent regarding issues for a prescriber during polypharmacy. The foremost objective of this research was to form a complex between linagliptin and rabeprazole sodium by in vitro interactions. The interactions between the drugs have been examined by monitoring some chromatographic and spectroscopic analyses viz. TLC, HPLC, FT-IR, UV, Job’s plot, conductometric titrations, and Ardon’s spectrophotometric strategy. Rabeprazole sodium formed a stable complex with linagliptin, which was ensured from the insight of these analytical data. The developed complex’s bright spot was clearly watched in the TLC plate. The retention time (Rt) of the formed complex was 5.303 min, where the Rt were 3.364 and 3.103 min for linagliptin and rabeprazole sodium, respectively, in HPLC chromatograms. In FT-IR and UV spectra of the formed complex revealed some disappearance of characteristic peaks that affirmed the complexation. All of the variations of the spectrophotometric and chromatographic properties from the antecedent drugs indicated the drug-drug interaction. Another crucial fact for the experimental aim was to affirm the assumed drug interaction by in vivo model examination. The assessment of anti-diabetic property on alloxan-induced Swiss albino mice proved significant in vivo interaction between the drugs. It was outlined from the animal study that the hypoglycemic activity of linagliptin might be significantly affected due to the complex formation of the drug with a proton pump inhibitor (PPI). Nonetheless, it is the primary outcome of the interaction, which recommends the bigger in vivo study or clinical monitoring on the human model.

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Antidiabetic activity of aqueous stem bark extract of Annickia polycarpa in alloxan-induced diabetic mice

Cited by 16 publications

References 29 publications

Ranunculus ternatus Thunb extract attenuates renal fibrosis of diabetic nephropathy via inhibiting SMYD2

Ranunculus ternatus Thunb extract attenuates renal fibrosis of diabetic nephropathy via inhibiting SMYD2

Effect Of Fruit And Cork Extract Of Ficus Lacor Buch Ham On α/β -Glucosidase, α -Amylase, Lipase, Glucose Absorption And Uptake

Does Rabeprazole Sodium Alleviate the Anti-diabetic Activity of Linagliptin? Drug-Drug Interaction Analysis by In vitro and In vivo Methods

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