Antidiabetic Activity of Hydromethanolic Extract of Crude Dorstenia barnimiana Root: Validation of In Vitro and In Vivo Antidiabetic and Antidyslipidemic Activity

Abstract

Background: Despite modern therapeutic armamentaria, DM remains a 21st-century public health menace. Novel phytomedicines are a rapidly expanding focus of research. The juice of Dorstenia barnimiana roots has long been used for the treatment of diabetes mellitus in traditional Ethiopian medicine, but its efficacy has not been supported by in vitro or in vivo scientific study. To investigate this, the present work was performed. Methods: In this experimental study, simple random sampling was applied. Healthy male mice were used in normoglycemic and oral glucose-tolerance test (OGTT) models. Streptozotocin (IP, 150 mg/kg)-administered diabetic male mice were utilized. Animals were randomly divided into five groups of six each. Group I received 10 mL/kg distilled water, groups II-IV received 100 (DB100), 200 (DB200), and 400 (DB400) mg/kg crude extract, respectively, and group V received glibenclamide 5 mg/kg. A sham group (group VI) was added that received 10 mL/kg distilled water. All treatments were given orally. FBG, serum-lipid profiles, and body-weight changes were then measured. In vitro α-amylase inhibitory activity was also evaluated. Results:The doses were atoxic up to 2,000 mg/kg. There was α-amylase inhibition activity of 67.52% at 500 μg/mL with an IC 50 of 4.595 μg/mL. The OGTT revealed an antihyperglycemic effect of the crude extract. This was not attributed to a hypoglycemic side effect. In the diabetic mouse model, it shrank FBG levels remarkably. There were also significant reductions in serum TC, TGs, VLDL-C, and LDL-C. Nevertheless, HDL-C and body-weight levels returned. Conclusion:The present study confirmed the safety and promising in vivo antidiabetic and antidyslipidemic activity of D. barnimiana, thus corroborating the traditional claim.

Luteolin-Rich Extract of Thespesia garckeana F. Hoffm. (Snot Apple) Contains Potential Drug-Like Candidates and Modulates Glycemic and Oxidoinflammatory Aberrations in Experimental Animals

“…The OGTT was conducted according to the method described by Sisay et al [ 43 ]. A total of twelve (12) overnight starved rats were grouped into 5 (3 rats per group) and treated with 2.0 mL/kg normal saline, 5 mg/kg glibenclimide, 150 mg/kg, and 300 mg/kg dietl-eth_ T. garckeana for groups I-IV, respectively.…”

Validation of Antidiabetic and Antihyperlipidemic Effects of 80% Methanolic Extract of the Lonchocarpus laxiflorus Leaves in Streptozotocin-Induced Diabetic Swiss Albino Mice

“…Group VI–The normal (nondiabetic) control group received vehicle (10 ml/kg 2% tween 80 in distilled water). The process of grouping and dosing was conducted according to the procedure [ 25 ].…”

“…The normal male mice that were fasted overnight (14 h) from food with free access to water were grouped randomly into five groups (six mice per group) and received their respective treatment as mentioned in Grouping and Dosing. The BGL was measured just before (0 h as baseline) administration of each treatment, and then, after at 1 st , 2 nd , 4 th , and 6 th h post-administration of the respective treatment [ 25 ].…”

“…The mice were administered 2 g/kg glucose solution orally (this matches with 10 μ l/g of fasted body mass in 20% glucose solution) after 30 minutes of administration of their respective treatment (the vehicle, the standard drug, and the crude extract). Then, the blood sample was taken from each mouse, and the BGL was measured just before loading of glucose solution (at 0 h as baseline), and at 30, 60, 90, and 120 minutes post-glucose load [ 25 ].…”

Glinus lotoides linn. Seed extract as antidiabetic agent: In vitro and in vivo anti-glucolipotoxicity efficacy in Type-II diabetes mellitus

“…In Ethiopia diabetic patient used medicinal plants at a higher rate in complement to modern therapy [ 6 ]. To cure diabetes, Ethiopian traditional healers employs several plants, including the roots of Dorstenia barnimiana [ 7 ] , flower and leaf of Hagenia Abyssinica [ 8 , 9 ] , seed of Glinus lotoides [ 10 ] and leaf latex of Aloe megalacantha [ 11 ] .…”