Antidiabetic-activity sulfated polysaccharide from Chaetomorpha linum: Characteristics of its structure and effects on oxidative stress and mitochondrial function

Qin, Ling; Yang, Yajing; Hao, Jiejie; He, Xiaoxi; Liu, Shan; Chu, Xiao; Mao, Wenjun

doi:10.1016/j.ijbiomac.2022.02.129

Cited by 14 publications

(6 citation statements)

References 51 publications

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“…Therefore, there is a need to study new approaches to managing and preventing diabetes in obese individuals based on natural ingredients that are more sustainable and inexpensive, and these should be investigated based on evidence and facts [32]. Reinforced by evidence from other studies, SPs extracted from green algae have been shown to effectively inhibit the aggregation of human islet amyloid polypeptide and demonstrate a strong antidiabetic activity [15], although this was of a different genus than Caulerpa. Until now, there have been no studies that have successfully reported on the antidiabetic activity of green algae SPs via their inhibition of carbohydrate hydrolyzing enzymes (α-glucosidase and α-amylase), something that has been accomplished in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Extracted SPs of Brazilian C. racemosa and cell lines of the SPs of the Chinese C. lentillifera have been associated with reduced inflammation in animal models [8,9]. SPs from Brazilian green algae have also exhibited roles in the suppression of fat accumulation, showcasing antiadipogenic activity effects [9], improved enzymatic antibacterial activity [10], neuroprotective potentials [11,12], modulation of immunity [13], antitumor activities [12,14], and hypoglycemic activity [15]. Anti-oxidative stress is important and intricately related to cancer, as with antioxidants it can fight free radicals and also suppress the growth of cancer cells.…”

Section: Introductionmentioning

confidence: 99%

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New Insight on In Vitro Biological Activities of Sulfated Polysaccharides from Ulvophyte Green Algae

et al. 2023

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Green algae are natural bioresources that have excellent bioactive potential, partly due to sulfated polysaccharides (SPs) which are still rarely explored for their biological activities. There is currently an urgent need for studies exploring the anticancer biological activity of SPs extracted from two Indonesian ulvophyte green algae: the sulfated polysaccharide of Caulerpa racemosa (SPCr) and the sulfated polysaccharide of Caulerpa lentillifera (SPCl). The method of isolating SPs and their assessment of biological activities in this study were based on previous and similar studies. The highest yield sulfate/total sugar ratio was presented by SPCr than that of SPCl. Overall, SPCr exhibits a strong antioxidant activity, as indicated by smaller EC50 values obtained from a series of antioxidant activity assays compared to the EC50 values of Trolox (control). As an anti-obesity and antidiabetic, the overall EC50 value of both SPs was close to the EC50 of the positive control (orlistat and acarbose). Even more interesting was that SPCl displayed wide-ranging anticancer effects on colorectal, hepatoma, breast cancer cell lines, and leukemia. Finally, this study reveals new insights in that SPs from two Indonesian green algae have the potential to be promising nutraceuticals as novel antioxidative actors, and to be able to fight obesity, diabetes, and even cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New Insight on In Vitro Biological Activities of Sulfated Polysaccharides from Ulvophyte Green Algae

et al. 2023

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“…Up to now, the report on the structural characteristics of sulfated polysaccharides from the genus Chaetomorpha was seldom found. Qin et al [ 21 ] isolated a sulfated rhamnogalactoarabinan from the green alga C. linum ; its backbone was composed of (1→4) and (1→3,4)-β- l -arabinopyranose with sulfate groups at C-2/C-3 of (1→4)-β- l -arabinopyranose, and the branches contained 4-linked, 6-linked β- d -galactopyranose and terminal rhamnose residues. Recently, arabinan-type sulfated polysaccharides from other green algae were reported.…”

Section: Resultsmentioning

confidence: 99%

“…The molecular weight was estimated by reference to a calibration curve made by pullulan standards. Monosaccharide composition was assayed by a 1-phenyl-3-methyl-5-pyrazolone (PMP)-HPLC method using the Eclipse XDB-C18 column (4.6 × 250 mm, Agilent Technologies, Santa Clara, CA, USA) [ 21 ]. Sugar configuration was determined by reversed-phase HPLC [ 32 ] and the analysis was performed on an Agilent 1260 Infinity HPLC instrument (Agilent Technologies, Santa Clara, CA, USA) using an Agilent XDB-UV detector (250 nm).…”

Section: Methodsmentioning

confidence: 99%

Anticoagulant Property of a Sulfated Polysaccharide with Unique Structural Characteristics from the Green Alga Chaetomorpha aerea

2023

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Sulfated polysaccharides from marine algae have attracted a great amount of attentions for the development of marine drugs due to their unique structural features, and they are great potential sources of naturally occurring anticoagulant agents. The genus Chaetomorpha is one of the largest genera in green algae and has a worldwide distribution. In the present study, a homogeneous polysaccharide from Chaetomorpha aerea, designated as PCA, was obtained by alkali extraction, anion-exchange and size-exclusion chromatography. Based on the results of chemical and spectroscopic analyses, PCA was a sulfated galactoarabinan which was mainly constituted of a backbone of →4)-β-l-Arap-(1→ unit, partially sulfated at C-3 of →4)-β-l-Arap-(1→ and C-4 of →6)-α-d-Galp-(1→. The side chains consisting of →6)-α-d-Galp-(1→ and →5)-α-l-Araf-(1→ residues were in C-2 of →4)-β-l-Arap-(1→ unit. PCA had a strong anticoagulant activity in vitro as evaluated by the assays of activated partial thromboplastin time, thrombin time and fibrinogen level. The obvious anticoagulant activity in vivo of PCA was also found. PCA significantly inhibited the activities of the intrinsic coagulation factors XII, XI, IX and VIII, and exhibited weak inhibition effects on the common coagulation factors II and X. The anticoagulant mechanism of PCA was attributed to strong thrombin inhibition potentiated by heparin cofactor II or antithrombin III, and it also possessed an apparent inhibition effect on coagulation factor Xa mediated by antithrombin III. The investigation demonstrated that PCA could be a promising anticoagulant agent for health promotion and the treatment of thrombotic diseases.

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“…Accumulating evidence indicates that the occurrence of IR is accompanied by downregulation of glucose consumption and metabolism, which results from oxidative stress due to the disturbance of the balance between reactive oxygen species (ROS) production and antioxidant defense mechanism. − Hence, it is not difficult to find that the elimination of oxidative stress is viewed as an effective strategy for ameliorating IR in T2DM. Previous studies evaluated common oxidation markers (ROS, superoxide dismutase (SOD), lactate dehydrogenase (LDH), malondialdehyde (MDA), and NO) to reveal mechanisms of oxidative stress. − Specifically, enhancing SOD activity and decreasing ROS level, LDH activity, MDA, and NO content play beneficial roles in eliminating oxidative stress. Taken together, the weakening of the high-glucose-induced oxidative stress was related tightly to ROS, SOD, LDH, MDA, and NO, implementing beneficial effects on redox homeostasis and insulin sensitivity. , …”

Section: Introductionmentioning

confidence: 99%

Assessment of Novel Oligopeptides from Rapeseed Napin (Brassica napus) in Protecting HepG2 Cells from Insulin Resistance and Oxidative Stress

Yao

et al. 2022

J. Agric. Food Chem.

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Oligopeptides (Thr–His–Leu–Pro–Lys (THLPK), His–Pro–Leu–Lys (HPLK), Leu–Pro–Lys (LPK), His–Leu–Lys (HLK), and Leu–His–Lys (LHK)) are newly identified from rapeseed napin (Brassica napus) protein-derived hydrolysates with the capability of upregulating glucose transporter-4 (GLUT4) expression and translocation. However, whether each of them enhances GLUT4 expression and translocation and their specific mechanisms remain unclear. Here, we assess the effects of the oligopeptides against insulin resistance (IR) and oxidative stress in hepatocytes and screen out the most antidiabetic one. Specifically, compared with other oligopeptides, LPK not only remarkably elevated glucose consumption to 8.45 mmol/L protein; superoxide dismutase (SOD) activity to 319 U/mg protein; GLUT4 expression and translocation; and phosphorylated level of insulin receptor substrate-1 (IRS-1), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (Akt) (P < 0.05) but also remarkably attenuated the reactive oxygen species (ROS) level to 2255, lactate dehydrogenase (LDH) activity to 20.5 U/mg protein, malondialdehyde (MDA) content to 241 nmol/mg protein, and NO content to 1302 μmol/mL protein (P < 0.05). These findings demonstrated that antidiabetic oligopeptide LPK possessed the most potential to protect HepG2 cells from IR and oxidative stress via activating IRS-1/PI3K/Akt/GLUT4 and regulating common oxidative markers in vitro.

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Antidiabetic-activity sulfated polysaccharide from Chaetomorpha linum: Characteristics of its structure and effects on oxidative stress and mitochondrial function

Cited by 14 publications

References 51 publications

New Insight on In Vitro Biological Activities of Sulfated Polysaccharides from Ulvophyte Green Algae

New Insight on In Vitro Biological Activities of Sulfated Polysaccharides from Ulvophyte Green Algae

Anticoagulant Property of a Sulfated Polysaccharide with Unique Structural Characteristics from the Green Alga Chaetomorpha aerea

Assessment of Novel Oligopeptides from Rapeseed Napin (Brassica napus) in Protecting HepG2 Cells from Insulin Resistance and Oxidative Stress

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