Antidiabetic Potential of Protein Hydrolysates and Peptide Fractions from Lima Bean (Phaseolus lunatus L): An In Vitro Study

Castañeda-Pérez, Eduardo; Jiménez-Morales, Karina; Ruelas, Arturo F. Castellanos; Chel‐Guerrero, Luis; Betancur‐Ancona, David

doi:10.1007/s10989-021-10226-8

Cited by 17 publications

(6 citation statements)

References 38 publications

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“…In the case of glutelin fraction, the increase was moderated, reaching a DH value for PPH of 20.07%. This value was similar to those reported for raw peanut protein hydrolyzed by pepsin for 30 min and pancreatin for 24 h (21.4%) [ 30 ], and for Phaseolus lunatus (lima bean) protein hydrolyzed by pepsin for 45 min and pancreatin for 45 min (22.09%) [ 31 ].…”

Section: Resultssupporting

confidence: 88%

Albumin from Erythrina edulis (Pajuro) as a Promising Source of Multifunctional Peptides

et al. 2021

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Multifunctional peptides, capable of acting on different body systems through multiple mechanisms of action, offer many advantages over monofunctional peptides, including lower adverse side effects and costs. Erythrina edulis (pajuro) is a legume with a large number of high-quality proteins, of which their potential as a source of antioxidant peptides has been recently reported. In this study, the behavior of these proteins under a sequential enzymatic hydrolysis with digestive and microbial enzymes was investigated by evaluating the multi-functionality of the hydrolyzates. The albumin hydrolyzates obtained after the action of pepsin, pancreatin, and Alcalase showed antioxidant, angiotensin-converting enzyme (ACE), α-amylase, α-glucosidase, and dipeptidyl peptidase (DPP)-IV inhibitory activities. The radical scavenging properties of the hydrolyzate could be responsible for the potent protective effects observed in FeSO4-induced neuroblastoma cells. The findings support the role of pajuro protein as an ingredient of functional foods or nutraceuticals for health promotion and the prevention of oxidative stress, hypertension, and metabolic alteration-associated chronic diseases.

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Section: Resultssupporting

confidence: 88%

Albumin from Erythrina edulis (Pajuro) as a Promising Source of Multifunctional Peptides

et al. 2021

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“…(2010) confirmed that MBP contains an α‐amylase inhibitor with a molecular weight of 27 kDa and a specific activity of 14.5 U/(mg of protein), lower than that of common bean (495.2–1925.5 U/(mg of protein)). In addition, MBP peptides obtained from Alcalase hydrolysis showed higher α‐glucosidase inhibitory activity (42.17%) (Li et al., 2022) than that obtained from lupin (22.61%) (Fadimu et al., 2022), yellow field pea ( Pisum sativum L.) (38.39%) (Awosika & Aluko, 2019), and common bean (15.04%) (Ohara et al., 2021) but showed lower than pinto Durango bean (76.4%) (Oseguera‐Toledo et al., 2015), Lima Bean ( Phaseolus lunatus L.) (69.4%) (Castaneda‐Perez et al., 2021), chickpea ( Cicer arietinum L.) (56%) (Quintero‐Soto et al., 2021), and black bean ( Phaseolus vulgaris L.) (66.1%) (Mojica & de Mejia, 2016). Meanwhile, the results showed that the fasting blood glucose level of the high‐fat diet mice decreased by 24.93% after 5 weeks of MBPs dietary intervention (245 mg/kg/day) (Li et al., 2022), which was significantly better than that of black soy protein peptide (about 3.9%, 450 mg of supplement/day for 12 weeks) (Kwak et al., 2010), but was lower than that of soybeans (about 34%, 47.5 mg/kg/day, 3 weeks) (Jiang, Yan, et al., 2018) and black bean (53%, 150 mg/kg BW/day, 10 days) (Mojica et al., 2017).…”

Section: Extraction Methods and Ingredients Characteristics Of Mbpmentioning

confidence: 99%

A comprehensive review of mung bean proteins: Extraction, characterization, biological potential, techno‐functional properties, modifications, and applications

Feng

Hao³

et al. 2023

Comp Rev Food Sci Food Safe

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The popularity of plant-based proteins has increased, and mung bean protein (MBP) has gained immense attention due to its high yield, nutritional value, and health benefits. MBP is rich in lysine and has a highly digestible indispensable amino acid score. Dry and wet extractions are used to extract MBP flours and concentrates/isolates, respectively. To enhance the quality of commercial MBP flours, further research is needed to refine the purity of MBPs using dry extraction methods. Furthermore, MBP possesses various biological potential and techno-functional properties, but its use in food systems is limited by some poor functionalities, such as solubility. Physical, biological, and chemical technologies have been used to improve the techno-functional properties of MBP, which has expanded its applications in traditional foods and novel fields, such as microencapsulation, three-dimensional printing, meat analogs, and protein-based films. However, study on each modification technique remains inadequate. Future research should prioritize exploring the impact of these modifications on the biological potential of MBP and its internal mechanisms of action. This review aims to provide ideas and references for future research and the development of MBP processing technology.

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“…The half-maximum inhibition concentration (IC 50 ) was calculated using nonlinear regression. The MGAM inhibitory activity was calculated as follows [39]:…”

Section: Mgam Inhibitionmentioning

confidence: 99%

Understanding Antidiabetic Potential of Oligosaccharides from Red Alga Dulse Devaleraea inkyuleei Xylan by Investigating α-Amylase and α-Glucosidase Inhibition

Mune Mune,

Hatanaka,

Kishimura

et al. 2024

Molecules

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In this study, the α-glucosidase (maltase-glucoamylase: MGAM) and α-amylase inhibitory properties elicited by xylooligosaccharides (XOSs) prepared from dulse xylan were analysed as a potential mechanism to control postprandial hyperglycaemia for type-2 diabetes prevention and treatment. Xylan was purified from red alga dulse powder and used for enzymatic hydrolysis using Sucrase X to produce XOSs. Fractionation of XOSs produced xylobiose (X2), β-(1→3)-xylosyl xylobiose (DX3), xylotriose (X3), β-(1→3)-xylosyl-xylotriose (DX4), and a dulse XOS mixture with n ≥ 4 xylose units (DXM). The different fractions exhibited moderate MGAM (IC50 = 11.41–23.44 mg/mL) and α-amylase (IC50 = 18.07–53.04 mg/mL) inhibitory activity, which was lower than that of acarbose. Kinetics studies revealed that XOSs bound to the active site of carbohydrate digestive enzymes, limiting access to the substrate by competitive inhibition. A molecular docking analysis of XOSs with MGAM and α-amylase clearly showed moderate strength of interactions, both hydrogen bonds and non-bonded contacts, at the active site of the enzymes. Overall, XOSs from dulse could prevent postprandial hyperglycaemia as functional food by a usual and continuous consumption.

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Antidiabetic Potential of Protein Hydrolysates and Peptide Fractions from Lima Bean (Phaseolus lunatus L): An In Vitro Study

Cited by 17 publications

References 38 publications

Albumin from Erythrina edulis (Pajuro) as a Promising Source of Multifunctional Peptides

Albumin from Erythrina edulis (Pajuro) as a Promising Source of Multifunctional Peptides

A comprehensive review of mung bean proteins: Extraction, characterization, biological potential, techno‐functional properties, modifications, and applications

Understanding Antidiabetic Potential of Oligosaccharides from Red Alga Dulse Devaleraea inkyuleei Xylan by Investigating α-Amylase and α-Glucosidase Inhibition

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