Antidiabetic potential of salvianolic acid B in multiple low-dose streptozotocin-induced diabetes

Abstract

Context: Salvianolic acids are the most abundant water-soluble compounds extracted from the herb Salvia miltiorrhiza L. (Lamiaceae) with antioxidant and protective effects. Objective: This study evaluates the antidiabetic effect of salvianolic acid B (Sal B) in multiple low-dose streptozotocin (MLDS)-induced diabetes in rat. Materials and methods: Rats were divided into control, Sal B40-treated control, diabetic, Sal B20-, and Sal B40-treated diabetic groups. Sal B was daily administered at doses of 20 or 40 mg/kg (i.p.), started on third day post-STZ injection for 3 weeks. Serum glucose and insulin level and some oxidative stress markers in pancreas were measured in addition to the oral glucose tolerance test (OGTT), histological assessment, and apoptosis determination. Results: After 3 weeks, treatment of diabetic rats with Sal B20 and Sal B40 caused a significant decrease of the serum glucose (p50.05-0.01) and improvement of OGTT. Meanwhile, serum insulin was significantly higher in Sal B20-and Sal B40-treated diabetics (p50.01) and treatment of diabetics with Sal B40 significantly lowered malondialdehyde (MDA) (p50.05), raised glutathione (GSH) (p50.05), and activity of catalase (p50.01) with no significant change of nitrite. Furthermore, the number of pancreatic islets (p50.05) and their area (p50.01) was significantly higher and apoptosis reactivity was significantly lower (p50.05) in the Sal B40-treated diabetic group versus diabetics. Discussion and conclusion: Three-week treatment of diabetic rats with Sal B exhibited antidiabetic activity which is partly exerted via attenuation of oxidative stress and apoptosis and augmentation of antioxidant system.

Salvianolic Acid B Ameliorates Hyperglycemia and Dyslipidemia in db/db Mice through the AMPK Pathway

“…More and more evidences indicate that Sal B exerts multiple beneficial effects on the metabolic syndrome and type 2 diabetes [17][18][19][20], although its extensive action mechanism is not clear. In this study, Sal B significantly decreased fasting blood glucose and lipid levels, and improved insulin sensitivity in db/db mice with similar effectiveness as metformin, which were consistent with our previous reports of Sal B in type 2 diabetic rats and dietinduced obese mice [17][18][19].…”

“…Although its most common use is in the treatment of cardio-cerebral vascular diseases [14][15][16], Sal B is recently reported to possess a number of potential implications in the treatment of insulin resistance, obesity, and type 2 diabetes [17][18][19][20]. Sal B or Salvia miltiorrhiza extract improved glycemic control, dyslipidemia, and insulin sensitivity in high fat diet and streptozocin-induced type 2 diabetic rats [17,19].…”

Herbal acupuncture for type 2 diabetes: A meta-analysis

“…It has been reported to reduce complications of diabetes such as increased urinary albumin excretion, plasma creatinine and blood urea nitrogen levels (28), as well as diabetic nephropathy (29,30). S. miltiorrhiza has also shown a significant anti-diabetic effect in animal studies; for instance, it decreases FBG and increases the insulin sensitivity index (31,32). It is effective for preventing and treating diabetic complications such as myocardial malfunction (33,34) and diabetic retinopathy (35).…”

Salvianolic acid B ameliorates hepatocyte lipid droplet accumulation via stimulation of autophagy

“…[11,12] Recent studies also reported a potential implication of Sal B in the treatment of insulin resistance, obesity, and type 2 diabetes. [13][14][15][16] In hepatocytes, Sal B has been reported a strong ability to protect cells from injury induced by oxidative stress, in ammation and enhance hepatic differentiation. [17][18][19] However, the effect of Sal B on improving hepatic lipid disorder is rarely reported.…”