Antidiabetic properties of dietary phenolic compounds: Inhibition effects on α‐amylase, aldose reductase, and α‐glycosidase

Abstract

Aldose reductase (AR), α‐amylase, and α‐glycosidase are vital enzymes to prevent diabetic complications. Here, AR was purified from sheep kidney using elementary methods with 111.11‐purification fold and with 0.85% purification yield. The interactions between some phenolic compounds and the AR, α‐glycosidase, and α‐amylase enzyme were determined. It was found that phenolic compounds exhibit potential inhibitor properties for these enzymes. For α‐amylase, studied phenolic compounds showed IC50 values in the range of 601.56–2,067.78 nM. For α‐glycosidase, Ki values were found in the range of 169.25 ± 27.22–572.88 ± 106.76 nM. For AR, Ki values in the range of 8.48 ± 0.56–43.26 ± 7.63 µM. However, genistein showed the best inhibition effect toward AR and α‐glycosidase, but delphinidin chloride exhibited the best inhibition effect against α‐amylase enzyme. We determined that all compounds showed noncompetitive inhibition effect against AR and α‐glycosidase. Also, studied phenolic compounds may be useful in the prevention or treatment of diabetic complications.

The effects of zingerone against vancomycin‐induced lung, liver, kidney and testis toxicity in rats: The behavior of some metabolic enzymes

“…According to other studies, phenolic compounds are showed inhibitory effects on AR and α-Gly enzymes activity. [5] This result is supported by the literature. According to the results, VCM administration may have increased oxidative stress in the kidney.…”

“…Aldose reductase (AR) is the rate-determining enzyme of polyol pathways, which possesses a significant role in the complications of diabetes, such as retinopathy, cardiovascular disease, neuropathy, cataract, and nephropathy. [5,6] Controlling blood glucose can prevent the improvement and progression of diabetic complications. AR inhibition is though oft as crucial prose for the prevention of diabetic complications.…”

A Novel Ag-N-Heterocyclic Carbene Complex Bearing the Hydroxyethyl Ligand: Synthesis, Characterization, Crystal and Spectral Structures and Bioactivity Properties

“…Additionally, the α-glycosidase inhibitory effect of Ag-NHC complex 1 was carried out using the p-nitrophenyl-D-glycopyranoside molecule (p-NPG) as the substrate according to the method of Tao et al [40] Samples of this work were prepared by dissolving as mg/mL. This assay was performed according to previous studies [41,42].…”

Determination of the inhibition profiles of pyrazolyl–thiazole derivatives against aldose reductase and α‐glycosidase and molecular docking studies

“…This pathway metabolizes the excess glucose, resulting in adverse impacts with raised mitogen-activated protein kinase, protein kinase C, oxidative, reductive, and glycative stress, and advanced glycation end products. [1][2][3] The polyol pathway in cells consists of two main enzymes. Aldose reductase (AR) is a monomeric oxidoreductase, which causes the reduction of glucose molecule to sorbitol using NADPH.…”