Antidiabetic screening of some Indonesian marine cyanobacteria collection

Abstract

Cyanobacteria have been known as a potential extracellular-polysaccharide (EPS) producer. The objective of this study was to screen the marine cyanobacteria as potential antidiabetic agents. The present investigation was designed to determine the antidiabetic activity of EPS, intracellular-polysaccharide (IPS) and biomass extracts from marine cyanobacteria isolates. 10 cyanobacteria isolates were cultivated in IMK medium, at 25 o C for 21 days. The morphology of cells was identified by a light microscope. EPS and IPS were separated by ethanol precipitation method and their antidiabetic activity was analyzed by the inhibition of α-glucosidase activity method. Results of morphology identification of 10 cyanobacteria isolates consist of Oscillatoria limnetica, Oscillatoria sp., Leptolyngbya sp., Pseudanabaena sp., Lyngbya sp. and Phormidium sp., Coelastrella sp., Aphanothece sp. and Synechococcus sp., and Chroococcus sp. Almost all of EPS from marine cyanobacteria isolates were potential as inhibitor of α-glucosidase, except for Oscillatoria limnetica and Phormidium sp. isolates. The highest activity in α-glucosidase inhibition was detected in Pseudanabaena sp. (14.02%) and Chroococcus sp. (13.0%) isolates.

Glucosidase Inhibitors Screening in Microalgae and Cyanobacteria Isolated from the Amazon and Proteomic Analysis of Inhibitor Producing Synechococcus sp. GFB01

“…Other α-glucosidase inhibitory compounds identified in cyanobacteria are present in biofilms [ 32 ], including the extra and intracellular polysaccharides [ 37 ], the latter with action well below that observed in methanolic extracts, with the greatest anti-α-glucosidase action, 14%, detected in extracellular polysaccharides from Pseudanabaena sp., in Synechococcus sp. ; these polysaccharides inhibited 3% of α-glucosidase, suggesting that the inhibitory activity of GFB01 might have another origin.…”

“…With occurrence in animals, plants and microorganisms [ 24 , 25 , 26 , 27 ], the bioprospection of glucosidase inhibitors in sustainable natural sources was once concentrated on macroalgae [ 28 , 29 , 30 ] but it is interesting to look for alternatives with cheaper cultivation and that require less area, such as microalgae, including cyanobacteria [ 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. The possibility of associating the production of these inhibitors with other metabolites of biotechnological interest produced by cyanobacteria, such as antioxidants [ 38 ]; pigments [ 39 ], acids and polysaccharides of cosmetic and pharmacological interest [ 40 , 41 ] in addition to joint production with polyhydroxybutyrate (PHB) [ 42 , 43 ] and biofuels [ 44 , 45 , 46 ], while mitigating CO 2 [ 43 , 44 ], is yet another advantage of this bacterial phylum.…”

“…The exact biological function of these inhibitors in cyanobacteria is still unknown, with defense against predators being the most discussed action [ 33 , 34 , 35 ]. The search for cyanobacteria that produce glucosidase inhibitors in environments with different physical and chemical conditions, and therefore, with different ecological relationships, is a good strategy to find biological compounds that are often still unexplored, such as the search for inhibitors in marine cyanobacteria the Baltic Sea [ 51 ], tropical environments [ 37 ] and flooded regions in the Nile delta [ 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 ].…”

Total Phenolic Content and in Vitro Analysis of Antioxidant, Antibacterial, and Alpha-Glucosidase Inhibition Properties of Chroococcus Minutus (Kützing) Nägeli (Chroococcales, Cyanobacteria)

“…Diabetes is a metabolic disease characterized by having high concentration of glucose in blood [21]. The management of this metabolic disorder is done by lowering the postprandial increase in blood glucose levels via inhibition of important carbohydrate-degrading enzyme (α-glucosidase), responsible for the breakdown of complex carbohydrates into glucose [22].…”

The Opportunity of Developing Microalgae Cultivation Techniques in Indonesia

“…and Pseudanabaena sp. produced higher yields than other marine cyanobacteria cultures, yielding biomass yields very low (Priatni et al, 2016). Seawater cultivation has also been carried out on Chaetoceros sp.…”