Antiedematogenic and antioxidant properties of high molecular weight protein sub-fraction of Calotropis procera latex in rat

Abstract

Objectives:The aim was to evaluate the effect of high molecular weight protein fraction of Calotropis procera latex on edema formation and oxidative stress in carrageenan-induced paw inflammation.Methods:A sub-plantar injection of carrageenan was given to induce edema in the hind paw of the rat. The inhibitory effect of high molecular weight protein fraction of C. procera latex was evaluated following intravenous administration (5 and 25 mg/kg body weight) and was compared with that of diclofenac given orally (5 mg/kg). The levels of reduced glutathione (GSH), thiobarbituric acid reactive substances (TBARS) and myeloperoxidase (MPO) were measured in the inflamed paw tissue at the end of the study.Results:The high molecular weight protein fraction obtained from the latex of C. procera produced a dose-dependent inhibition of edema formation that was accompanied by normalization of levels of oxidative stress markers (GSH and TBARS) and MPO, a marker for neutrophils in the paw tissue.Conclusions:The high molecular weight protein fraction of C. procera latex ameliorates acute inflammation in the paw through its antioxidant effect.

The Preventive Effect on Ethanol-Induced Gastric Lesions of the Medicinal PlantPlumeria rubra: Involvement of the Latex Proteins in the NO/cGMP/KATPSignaling Pathway

“…Latex fluids have been reported to display numerous pharmacological properties. This is in good agreement with the traditional and folk medicinal use of latex-bearing plants worldwide [ 6 , 7 ]. Plumeria rubra is a laticifer plant, commonly known as the frangipani or temple tree and distributed mainly in tropical and subtropical regions [ 8 ].…”

“…In our previous findings, the soluble proteins extracted from the latex (PrLP) showed antioxidant and proteolytic activity [ 17 ] and endothelial relaxation of rat thoracic aortic rings [ 18 ]. Furthermore, studies with the proteins extracted from latex of other plants have suggested that the proteins are strongly associated with the pharmacological properties claimed by the popular medicine [ 6 , 7 ]. In the present study, the aqueous protein fraction extracted from the latex of P .…”

Protective effect of high molecular weight protein sub-fraction of Calotropis procera Latex in Monoarthritic Rats

“…Recently, this fraction has been shown to inhibit edema formation in the rat model of acute inflammation. [ 14 ]…”

“…The anti-infiltrative and anti-edematogenic property of latex proteins have been well established in animal models. [ 14 15 ] The activated neutrophils are known to contribute to arthritic conditions by the release of inflammatory mediators, oxygen radicals, and tissue degrading enzymes. In addition, neutrophils synthesize eicosanoids and pro-inflammatory cytokines that eventually degrade the matrix components.…”

“…[ 13 ] Further segregation of these proteins by ion-exchange chromatography provided a sub-fraction as peak I (LP PI ) comprising high molecular weight proteins that have been shown to alleviate edema formation and neutrophilic influx in acute inflammation. [ 14 15 ] The present study was carried out to evaluate the efficacy of these proteins in ameliorating joint inflammation and dysfunction in a rat model of monoarthritis.…”