Antiedematogenic Evaluation ofCopaifera langsdorffiiLeaves Hydroethanolic Extract and Its Major Compounds

Abstract

Inflammatory disorders affect many people worldwide, and medicinal plants are used to ameliorate these health problems. This paper reports the antiedematogenic and analgesic evaluation of Copaifera langsdorffii Desf. leaves hydroethanolic extract (Cop) and two of its isolated compounds: quercetin-3-O-α-l-rhamnopyranosyl (quercitrin) and kaempferol-3-O-α-l-rhamnopyranosyl (afzelin). For that, the following experimental protocols were undertaken locomotor performance, writhing induced by acetic acid, antinociceptivity induced by formalin, hot plate latency, paw oedema induced by carrageenan and dextran, and cell migration induced by lipopolysaccharide (LPS), as well as the measurement of nitric oxide (NO), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 10 (IL-10) in macrophages. Neither the extract nor the isolated compounds displayed analgesic activity. The obtained results showed that C. langsdorffii extract possesses antiedematogenic properties acting on peripheral sites, whereas quercitrin and afzelin are not involved. Moreover, these properties are not associated with cell migration inhibition, TNF-α, IL-6, or IL-10 regulation.

Anti-inflammatory and antinociceptive effects of kaempferide from the Brazilian green propolis

“…In the carrageenan-induced paw edema test (Figure 3), it is observed that indomethacin significantly reduced the volume of the edema, as expected (Furtado et al, 2015;dos Santos et al, 2010). The dose of 10 mg/kg was significantly different from the negative control in the fourth hour of evaluation, showing the anti-inflammatory potential of kaempferide.…”

“…Thus, the effect of kaempferide may be correlated with inhibition on modulatory pathways of the arachidonic acid cascade, such as prostaglandins, cyclooxygenase products, and nitric oxide (NO). On the other hand, it is not correlated to mediators such as histamine, serotonin, and bradykinin on vascular permeability (Furtado et al, 2015). The modulation on inflammatory pathways of green propolis extract is reported in the literature, and the extract rich in artepillin C and kaempferide modulates the expression of different mediators, such as NO and several cytokines (Szliszka et al, 2013).…”

“…The number of crosses by the four legs was determined in 6-minute sessions and taken as a behavioral index. The animals were subsequently observed in the first four hours and signs of general toxicity, such as effects on locomotion, behavior (agitation, lethargy and aggression), breathing, salivation, tearing, cyanosis of the extremities, piloerection and complications were noted (Furtado et al, 2015;Walsh and Cummins, 1976).…”

Experimental skin wound treatment with Copaifera langsdorffii Desf Kuntze (Leguminosae) extract and oil-resin in horses

“…Copaifera langsdorffii is particularly important in Brazil because it is widely distributed throughout the country and is used in popular and indigenous knowledge as a healing and anti-inflammatory agent (Corrêa, 1984;Veiga & Pinto, 2002). Due to its use in popular medicine since the 16 th century, studies have reported several biological activities related to the Copaifera langsdorffii oil-resin and leaves extract, as well as skin wound healing (Gushiken et al, 2017a;Lemos et al, 2015;Masson-Meyer et al, 2013), anti-microbial activity (Abrão et al, 2015), gastrointestinal diseases treatments (Gomes et al, 2007;Lemos et al, 2015;Paiva et al, 2002), antiedematogenic (Furtado et al, 2015), antinociceptive (Gomes et al, 2007) and urolithiasis treatment (Brancalion et al, 2012;Oliveira et al, 2013). However, there are few studies reporting copaiba effects in horse skin wound healing (Lucas et al, 2017).…”

Avaliação dos efeitos citoprotetores de compostos isolados de Copaifera langsdorffii Desf. contra citotoxidade induzida pela exposição ao metilmercúrio e ao chumbo

“…O extrato hidroalcoólico das folhas também reduziu a formação de edema em dois modelos de estudo, cujo efeito anti-inflamatório foi associado à regulação de mediadores como a serotonina, e à liberação de óxido nítrico (Furtado et al 2015).…”