Anti-inflammatory and antinociceptive effects of kaempferide from the Brazilian green propolis

Abstract

The Brazilian green propolis is produced by Apis mellifera from Baccharis dracunculifolia. Kaempferide, which was isolated from green propolis and B. dracunculifolia is an O-methylated flavonol bearing gastroprotective, anti-allergic and anti-hypertensive activities. Thus, the aim of this study was to evaluate the anti-inflammatory and antinociceptive potentials of kaempferide, as well as its possible toxicological effect on kidney and liver. For that, nociception was assessed by thermal (hargreaves apparatus) and mechanical (Dynamic Plantar Aesthesiometer) sensitivities; anti-inflammatory potential was assessed by carrageenan induced edema; and the toxicological profile was assessed by blood biochemical assessment. Kaempferide significantly reduced mechanical nociception and displayed anti-inflammatory effect, which was evidenced by the reduction of the volume of the paw edema, as well as by the reduction of infiltrates, cellularity and area of the injured tissue. On the other hand, it was inactive in the thermal nociception protocol either with or without an inflammatory process, as well as it was inactive in the mechanical nociception protocol after an inflammatory process. In addition, central nervous system depression, liver and kidney toxicities were not observed.

Evaluación preliminar in vivo de la actividad analgésica de las flores de Brugmansia arborea “floripondio”

“…También se ha reportado que el ácido epi-ursólico presente en hojas de Cestrum buxifolium produjo efecto analgésico en las pruebas de ácido acético y de cola retirada (Ciangherotti et al, 2019). Más importante aún, es el hecho que varios metabolitos presentes en B. arborea (Kim et al, 2020), ya fueron reportados por su actividad analgésica ((De Melo et al, 2009, Toker et al, 2004, Almeida-Junior et al, 2020. ).…”

“…Con base en estudios fitoquímicos (Mattioli et al, 2012;Kim et al, 2020;Algradi et al, 2021) realizados a la especie (Brugmansia arborea) se han encontrado alcaloides tales como: la atropina, hioscina, noratropina (Arteaga de García et al, 1993), hiosciamina y escopolamina (Mattioli et al, 2012), de los que en algunos casos ya se les atribuye una actividad analgésica y antiinflamatoria (Addams & Wang, 2015). Del mismo modo, Kim et al, (2020) determinaron la presencia de flavonoides como kaempferin, kaempferitrin, kaempferol, quercetin, y (+)-aromadendrin; varios de los cuales ya fueron reportados por su efecto antinociceptivo (De Melo et al, 2009, Toker et al, 2004, Almeida-Junior et al, 2020.…”

Antimicrobial Formulation of a Bacterial Nanocellulose/Propolis-Containing Photosensitizer for Biomedical Applications

“…This product, which is originally used to fix and protect honeycombs, has also been recognized for centuries in terms of its biological and pharmacological properties [ 22 , 23 ]. Propolis extracts have been widely used in medicine due to their antiseptic, antimicrobial [ 24 , 25 ], anti-inflammatory, immunomodulatory [ 26 , 27 ], antioxidant [ 28 ], analgesic [ 27 ], antitumoral [ 29 ], and anticancer [ 30 ] properties, as well as wound-healing activities [ 24 , 31 ], among others. Previous studies have demonstrated the efficacy of free-standing BNC films containing standardized propolis extract (EPP-AF ® ), ethanol extract [ 20 ], and EPP-AF ® microemulsions [ 32 ] against Staphylococcus aureus and S. epidermidis bacteria, further confirming the healing effect of these composites on wounds.…”

Advances in the phytochemical screening and biological potential of propolis

“…Natural products play an important role in the treatment of the most diverse pathologies, since the allopathic substances available on the market have adverse effects, especially when it comes to anti-inflammatory drugs, such as irritation and ulceration in the gastric mucosa [1]. The search for natural substances with biological/pharmaceutical potential has been increasing among the pharmaceutical industries since 1960, being stimulated by the sale of products that are estimated at several billion dollars [2].…”