Antiepileptic activity of total triterpenes isolated fromPoria cocosis mediated by suppression of aspartic and glutamic acids in the brain

Abstract

Context: Triterpenes from Poria cocos Wolf (Polyporaceae) have been used to treat various diseases in traditional Chinese medicine. However, the antiepileptic effects and mechanism are not fully understood. Objective: The objective of this study is to investigate the antiepileptic properties of total triterpenes (TTP) from the whole P. cocos. Materials and methods: The ethanol extract TTP was identified by HPLC fingerprint analysis. Male ICR mice were gavaged (i.g.) with TTP (5, 20, 80 or 160 mg/kg) or reference drugs twice a day for 7 d. Antiepileptic activities of TTP were evaluated by maximal electroshock (MES)-and pentylenetetrazole (PTZ)-induced seizures in mice for 30 and 60 min, respectively. Locomotor activity and Rota-rod tests were performed for 60 min and 5 min, respectively. The levels of glutamic acid (Glu), aspartic acid (Asp), c-aminobutyric acid (GABA) and glycine (Gly) in convulsive mice were estimated. The chronic epileptic model of Wistar rats was built to measure expressions of glutamate decarboxylase 65 (GAD65) and GABA A in rat brain after TTP treatment. Results: The LC 50 of TTP (i.g.) was above 6 g/kg. TTP (5-160 mg/kg) protected mice against MES-and PTZinduced convulsions at 65.0% and 62.5%, respectively, but have no effect on rota-rod treadmill; TTP (20-160 mg/kg) significantly reduced the locomotor activities, shortened the onset of pentobarbital sodium-induced sleep; TTP decreased Glu and Asp levels in convulsive mice, but increased the GAD65 and GABA A expressions in chronic epileptic rats at doses usage. Discussion and conclusion: TTP extracted from P. cocos possessed potential antiepileptic properties and is a candidate for further antiepileptic drug development. ARTICLE HISTORY

Dose-Dependent Behavioral and Antioxidant Effects of Quercetin and Methanolic and Acetonic Extracts fromHeterotheca inuloideson Several Rat Tissues following Kainic Acid-InducedStatus Epilepticus

“…The B-ring in flavonoids, which is rich in hydroxyl groups, reacts with superoxide [49]. Some studies have shown that some terpenes regulate the glutamate decarboxylase expression and aspartic and glutamic acid levels in the brain [50] and that the GABA agonist capacity of some flavonoids is related to hydroxyl positions [51]. These different mechanisms could explain the effects of the main metabolites in acetonic and methanolic extracts of H. inuloides in attenuating seizures in epileptic rats.…”

Comparative Analysis of the Characteristics of Triterpenoid Transcriptome from Different Strains of Wolfiporia cocos

“…[5,6,7]. Modern studies have shown that triterpenoids in W. cocos have diverse pharmacological effects, such as anti-epilepsy, anti-apoptosis, diuresis, cytotoxicity, liver protection, anti-leukemia, anti-inflammatory, and so on [8,9,10,11,12,13,14,15,16]. Therefore, W. cocos has a wide and important role in future medical application.…”

Identification of geographical origin and different parts of Wolfiporia cocos from Yunnan in China using PLS‐DA and ResNet based on FT‐NIR

“…As a kind of medicine food homologous fungus, on the one hand, it is used to treat diseases, such as edema, insomnia, spleen deficiency, and vomiting 2 . Modern researches showed that the main active compositions were polysaccharides and triterpenes in W. cocos , which possessed a wide range of pharmacological activities including anti‐tumour, anti‐inflammatory, anti‐oxidant, anti‐bacterial, hepatoprotective, etc 3–8 . On the other hand, it is used as a food material, as early as the Qing Dynasty, such as Poria cakes.…”