Antiepileptogenic, antioxidant and genotoxic evaluation of rosmarinic acid and its metabolite caffeic acid in mice

Coelho, Vanessa Rodrigues; Vieira, Caroline Gonçalves; Souza, Luana Pereira de; Moysés, Felipe dos Santos; Basso, Carla; Papke, Débora Kuck Mausolff; Pires, Thienne Rocha; Siqueira, Ionara Rodrigues; Picada, Jaqueline Nascimento; Pereira, Patrícia

doi:10.1016/j.lfs.2014.11.009

Cited by 72 publications

(39 citation statements)

References 54 publications

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“…In this study, we demonstrated that RA reduces DNA damage in N9 cells, probably by inhibition of AIF translocation into the nucleus and attenuation of caspase-3 activation. These results corroborate other findings of our research group, when we used the comet assay and found that pretreatment with RA was able to minimize significantly the DNA damage in the brain cortex produced by PTZ-induced kindling in mice besides decreasing the production of ROS [27] .…”

supporting

confidence: 92%

“…1 ) is a natural hydroxylated phenolic compound present in many plants, including Rosmarinus officinalis , Artemisia capillaris , Callendula officinalis , Melissa officinalis , Salvia officinalis , perillae herba, and several other plant families [25] . Several biological activities are attributed to RA, such as antioxidant and anti-inflammatory properties and neuroprotective effects [26][27][28][29][30] . RA has been proven to be effective in experimental models of neurodegenerative diseases such as Alzheimer disease and amyotrophic lateral sclerosis [31,32] , and recently it was shown to have the interesting effect of reducing seizures in rats [27,[33][34][35] .…”

mentioning

confidence: 99%

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Rosmarinic Acid Attenuates the Activation of Murine Microglial N9 Cells through the Downregulation of Inflammatory Cytokines and Cleaved Caspase-3

Coelho

Viau²,

Staub³

et al. 2017

Neuroimmunomodulation

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Objective: The present study evaluated the ability of rosmarinic acid (RA) to inhibit microglia activation induced by lipopolysaccharide (LPS) in the N9 murine microglial cell line, and investigated the putative mechanisms involved in this process. Methods: In all tests, N9 murine microglial cells were pretreated with RA (0.1, 1.0, and 10 μM) for 20 h and exposed to LPS (1 μM/mL) for 4 h. Cell viability was measured by Trypan blue exclusion assay. Flow cytometry was used to detect reactive oxygen species (ROS), quantify cleaved caspase-3, and analyze the mitochondrial electrochemical potential. iNOS, Arg-1, TNF-α, IL-1β, and IL-6 proteins were analyzed by Western blotting, and their antigens were detected using the chemiluminescence technique. The effect of RA on DNA was evaluated by the Comet assay. Results: RA attenuated the expression of the M1 marker iNOS and the levels of proinflammatory factors, including TNF-α, IL-1β, and IL-6; it increased the expression of the M2 marker Arg-1, and inhibited, at least in part, ROS generation and loss of mitochondrial outer membrane permeabilization through the inhibition of cleaved caspase-3 activation. RA also inhibited DNA damage, reassuring cell protection. Conclusions: The results suggested a protective effect of RA through downregulation of inflammatory cytokines and cleaved caspase-3.

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supporting

confidence: 92%

mentioning

confidence: 99%

Rosmarinic Acid Attenuates the Activation of Murine Microglial N9 Cells through the Downregulation of Inflammatory Cytokines and Cleaved Caspase-3

Coelho

Viau²,

Staub³

et al. 2017

Neuroimmunomodulation

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“…In Figure , the total ion current (TIC) chromatogram (A) and UV chromatogram (B) at 280 nm of the investigated extract is presented. Its main constituents are carvacrol, naringenin, apigenin, eriodictyol, aromadedrin, and rosmarinic acid that exert antioxidative effects (Yanishlieva and others ; Rossato and others ; Annadurai and others ; Suh and others , Coelho and others ) and some of them possess antiinflammatory effects (Zhang and others ; Arigesavan and Sudhandiran ; Nasr‐Bouzaiene and others ; Rocha and others ). Therefore, EAO could hypothetically protect from autoimmune disease development by altering the immune response and preventing oxidative damage.…”

Section: Resultsmentioning

confidence: 99%

Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin‐Induced Diabetes in C57BL/6 Mice

Vujičić

Nikolić

Kontogianni

et al. 2016

Journal of Food Science

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Type 1 diabetes (T1D) is an autoimmune disease that develops as a consequence of pancreatic β-cell death induced by proinflammatory mediators. Because Origanum vulgare L. ssp. hirtum (Greek oregano) contains antiinflammatory molecules, we hypothesized that it might be beneficial for the treatment of T1D. An ethyl acetate extract of oregano (EAO) was prepared from the leaves by a polar extraction method. Phytochemical composition was determined by liquid chromatography-UV diode array coupled to ion-trap mass spectrometry with electrospray ionization interface (LC/DAD/ESI-MS(n) ). In vitro immunomodulatory effect of EAO was estimated by measuring proliferation (MTT) or cytokine secretion (ELISA) from immune cells. Diabetes was induced by multiple low doses of streptozotocin (MLDS) in male C57BL/6 mice and EAO was administered intraperitoneally for 10 d. Determination of cellular composition (flow cytometry) and cytokine production (ELISA) was performed on 12th d after diabetes induction. EAO suppressed the function of both macrophages and lymphocytes in vitro. In vivo, EAO treatment significantly preserved pancreatic islets and reduced diabetes incidence in MLDS-challenged mice. Besides down-modulatory effect on macrophages, EAO reduced the number of total CD4(+) and activated CD4(+) CD25(+) T cells. Furthermore, EAO affected the number of T helper 1 (Th1) and T helper 17 (Th17) cells through downregulation of their key transcription factors T-bet and RORγT. Because EAO treatment protects mice from development of hyperglycemia by reducing proinflammatory macrophage/Th1/Th17 response, this plant extract could represent a basis for future diabetes therapy.

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“…[6][7][8][9] However, to the best of our knowledge, there are no reports about the potential effects of caffeic acid, myristicin and rosemarinic acid on the gene expression and production of mucin from airway epithelial cells. Among the twenty one or more MUC genes coding human mucins reported up to now, MUC5AC was mainly expressed in goblet cells in the airway surface epithelium.…”

Section: -4mentioning

confidence: 99%

Effects of Caffeic Acid, Myristicin and Rosemarinic Acid on the Gene Expression and Production of Airway MUC5AC Mucin

Lee

Hong

et al. 2016

Nat Prod Sci

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− Perilla frutescens was empirically used for controlling airway inflammatory diseases in folk medicine. We investigated whether caffeic acid, myristicin and rosemarinic acid derived from Perilla frutescens significantly affect the gene expression and production of mucin from airway epithelial cells. Confluent NCI-H292 cells were pretreated with caffeic acid, myristicin or rosemarinic acid for 30 min and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 h. The MUC5AC mucin gene expression and production were measured by reverse transcription -polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Additionally, we examined whether caffeic acid, myristicin or rosemarinic acid affects MUC5AC mucin production indued by epidermal growth factor (EGF) and tumor necrosis factor-α (TNF-α), the other two stimulators of production of airway mucin. The results were as follows: (1) Caffeic acid, myristicin and rosemarinic acid inhibited the gene expression and production of MUC5AC mucin induced by PMA from NCI-H292 cells, respectively; (2) Among the three compounds derived from Perilla frutescens, only rosemarinic acid inhibited the production of MUC5AC mucin induced by EGF or TNF-α, the other two stimulators of production of airway mucin. These results suggest that rosemarinic acid derived from Perilla frutescens can regulate the production and gene expression of mucin, by directly acting on airway epithelial cells and, at least in part, explains the traditional use of Perilla frutescens as remedies for diverse inflammatory pulmonary diseases.

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Antiepileptogenic, antioxidant and genotoxic evaluation of rosmarinic acid and its metabolite caffeic acid in mice

Cited by 72 publications

References 54 publications

Rosmarinic Acid Attenuates the Activation of Murine Microglial N9 Cells through the Downregulation of Inflammatory Cytokines and Cleaved Caspase-3

Rosmarinic Acid Attenuates the Activation of Murine Microglial N9 Cells through the Downregulation of Inflammatory Cytokines and Cleaved Caspase-3

Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin‐Induced Diabetes in C57BL/6 Mice

Effects of Caffeic Acid, Myristicin and Rosemarinic Acid on the Gene Expression and Production of Airway MUC5AC Mucin

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