Antiestrogenic and Anti-Inflammatory Potential of n-Hexane Fraction of Vitex negundo Linn Leaf Extract: A Probable Mechanism for Blastocyst Implantation Failure in Mus musculus

Jivrajani, Mehul; Ravat, Nirav M; Anandjiwala, Sheetal; Nivsarkar, Manish

doi:10.1155/2014/241946

Cited by 5 publications

(5 citation statements)

References 25 publications

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“…anti-implantation activity(Jivrajani, Ravat, Anandjiwala, & Nivsarkar, 2014). These are supportive of the reversal of cellular multiplication mediated by extracts of VN.…”

supporting

confidence: 53%

“…The ethanolic extract is cytotoxic to HepG2 cells, its antioxidant activity protects ongoing thioacetamide‐induced nephrotoxicity in preclinical studies which validates the effect biochemically and morphologically. The proapoptotic effect is exemplified by its necrotic effect on tubular epithelial cells as well as by its antiestrogenic and anti‐implantation activity (Jivrajani, Ravat, Anandjiwala, & Nivsarkar, 2014). These are supportive of the reversal of cellular multiplication mediated by extracts of VN.…”

Section: Methodsmentioning

confidence: 99%

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Traditional medicine herbs as natural product matrices in cancer chemoprevention: A trans pharmacological perspective (scoping review)

Kalachaveedu

Senthil

Azhagiyamanavalan

et al. 2023

Phytotherapy Research

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Emerging evidence on molecular biology related to tumors, inflammation, and immunity, highlights their architectural commonality shifting cancer treatment paradigms toward more economical prevention than treatment. Statistical surveys reveal exponentially growing herbal drug supplementation in cancer worldwide as vast pre‐clinical and clinical data unravel their multi‐mechanistic pharmacology. The integrative oncological approach calls for more “holistic” principles to be amalgamated into cancer care. New cancer drug development from herbs need not be limited by the archetypal ‘RCT‐Standardization’ bottlenecks. Based on comprehensive literature scoping as per Prisma‐ScR guidelines, we herein concurrently reviewed evidence‐based research reports of selected Indian Traditional Medicine (ITM) herbs of anticancer repute in parallel with their holistic therapeutics; a rationalistic exploration of ITM's scientific genre. Their synergy effect on cancer revisited using a trans‐pharmacological approach validates ITM's seemingly simplistic health/disease equation model, showing a fresh new avenue for re‐purposing whole herbal drug complexes in cancer management. Herbal drugs as per ITM are natural matrices whose dynamics of interaction in the etiopathology of cancer are conceptually and mechanistically integrative. Lateral perspective to the same as laid out in this review holds the key to their effectual development as more tangible cancer chemopreventives/new drug targets/leads if not as new pharmacological tools.

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“…anti-implantation activity(Jivrajani, Ravat, Anandjiwala, & Nivsarkar, 2014). These are supportive of the reversal of cellular multiplication mediated by extracts of VN.…”

supporting

confidence: 53%

Section: Methodsmentioning

confidence: 99%

Traditional medicine herbs as natural product matrices in cancer chemoprevention: A trans pharmacological perspective (scoping review)

Kalachaveedu

Senthil

Azhagiyamanavalan

et al. 2023

Phytotherapy Research

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“…Hence, SOD and superoxide anion radical were used as a marker for blastocyst implantation. [ 23 34 ] Estrogen surge at the time of implantation has been shown to be responsible for the decrease in SOD levels and increase in superoxide anion radical levels (as shown in the control animals). [ 32 ] However, this pattern was altered in the n -hexane extract treated animals were SOD levels increase and radical levels fall significantly at the time of implantation.…”

Section: Resultsmentioning

confidence: 99%

“…For instance, n -hexane fraction of Vitex negundo Linn (Verbenaceae) leaves inhibit blastocyst implantation in mice when administered orally on days 1–6 of pregnancy. [ 23 ] V. negundo is very well known for its anti-inflammatory activity. Similarly, leaf extract of Hibiscus rosa-sinensis is also reported to possess anti-implantation activity.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory, Anti-estrogenic, and Anti-implantation activity of Bergia suffruticosa (Delile) Fenzl

Bind¹,

Jivrajani²,

Anandjiwala³

et al. 2015

Phcog Mag

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Background:Bergia suffruticosa (Delile) Fenzl (Syn. Bergia odorata Edgew) (Elatinaceae family) is used traditionally to repair bones and is applied as a poultice on sores. It is also used for stomach troubles and as an antidote to scorpion stings. So far, very little scientific work has been reported to validate its ethnomedical uses in the alleviation of pain, bone repair, etc.,Objective:This study was designed to explore the anti-inflammatory and anti-implantation potential of n-hexane extract of B. suffruticosa whole plant in mice along with identification of its chemical constituents.Materials and Methods:n-Hexane extract of B. suffruticosa whole plant was screened for acute and chronic anti-inflammatory activity followed by an anti-estrogenic activity. Eventually, n-hexane extract was tested for anti-implantation activity by exploiting markers of uterine receptivity, lipid peroxidation, and superoxide enzyme activity. The extract was administered orally at a dose of 100 mg/kg body weight in each study.Results:Thin layer chromatography fingerprint profile of n-hexane extract revealed the presence of lupeol and β-sitosterol. The n-hexane extract reduced the edema by 80% in acute inflammation, whereas it reduced edema to 75% on the 5th day in chronic inflammation. The n-hexane extract reduced elevated malonaldehyde level from 6 to 2.5 nmol/g × 10−5 and increased superoxide dismutase enzyme activity from 0 to 350 units/g in treated animals on the 5th day of pregnancy. Moreover, extract decreased uterine weight from 0.33 to 0.2 g in estradiol treated animals.Conclusion:These results indicate that n-hexane extract of B. suffruticosa is having potent anti-inflammatory, anti-estrogenic, and anti-implantation activity. This is the first report of all the pharmacological activities of B. suffruticosa mentioned above.SUMMARY TLC fingerprint profile of n-hexane extract of Bergia suffruticosa whole plant revealed the presence of lupeol and β-sitosteroln-Hexane extract showed in vivo anti-inflammatory activity in both acute and chronic model of inflammation in ratsn-Hexane extract possess significant anti-estrogenic activityn-Hexane extract altered the levels superoxide anion radical and superoxide dismutase enzyme activity during the blastocyst implantationAnti-implantation activity of n-hexane extract is attributed to its anti-inflammatory and anti-estrogenic potential. Abbreviations used: TLC: Thin layer chromatography; LPO: Lipid peroxidation; SOD: Superoxide dismutase; B. suffruticosa: Bergia suffruticosa; TNF-α: Tumor necrosis factor-α; NO: Nitric oxide; IL-1: Interleukin-1; LIF: Leukemia inhibitory factor; CSF-1: Colony-stimulating factor; COX: Cyclooxygenase; SDS: Sodium dodecyl sulfate; IAEC: Animal House Ethics Committee; CPCSEA: Committee for the Purpose of Control and Supervision of Experiments on Animals; HBSS: Hank's balanced salt solution; MDA: Malonaldehyde; and TBA: Thiobarbituric acid.

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“…Ethanolic extract of leaves (at two different doses -100 mg/kg and 300 mg/ kg body weight) administered for 12 weeks showed hepatoprotective effect against thioacetamide-induced hepatic injury in Sprague Dawley rats 78,79 . n-hexane extract of leaves of V. negundo when administered for day 1-6 of pregnancy in female Swiss albino mice showed failure of blastocyte implantation highlighting its antiestrogenic potential 80 . The essential oil of Vitex negundo was found efficacious in inhibiting COX-2 pathway and thereby reducing inflammation, pain and arthritis.…”

Section: In Vivo Studiesmentioning

confidence: 96%

A Systematic Review on the Pharmacological Prospectives of Vitex negundo in Ayurveda Drug Research

Devipriya,

Mohan,

Mohanan

et al. 2023

JNR

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In Ayurveda, there are many formulations containing Vitex negundo which is indicated in specific pathogenic and non-pathogenic condition. Hence this review attempted to explore the data of research at a glimpse. Peer reviewed and published data from PubMed had been selected for the same. The PubMed database (2009 to 2019) were searched using the keyword ‘Vitex negundo’, ‘Ayurveda’ with Boolean operator ‘AND’. A total of 45 free full text articles were retrieved from 57 articles with specific inclusion criteria. All the 45 studies were categorized into in silico studies, analytical studies, in vitro studies, in vivo studies and clinical studies. Then the data such as the type and design of research, the techniques used, and sample size were categorized into tables and the results were attained through thorough reading and analysis. A descriptive method is adopted for the review and quality aspects of the data were not taken into consideration. Compounds namely, acerosin and thymol were studied in in silico as an inhibitor for mTOR and on skin diseases respectively. Analytical studies explored the presence of compounds such as estriol, angnuside, phydroxybenzoicacid etc. Pharmacological activities such as anti-bacterial, anti-cancerous, anti-tubercular were studied in vitro and in vivo. A clinical study on Nirgundi khanavati in gridrasi (sciatica) is found to be effective in reducing the pain threshold. The phytochemical research of the drug were also proven against carcinoma. There is a high scope for research for more clinical studies and standardization with advanced analytical procedures and marker compounds.

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Antiestrogenic and Anti-Inflammatory Potential of n-Hexane Fraction of Vitex negundo Linn Leaf Extract: A Probable Mechanism for Blastocyst Implantation Failure in Mus musculus

Cited by 5 publications

References 25 publications

Traditional medicine herbs as natural product matrices in cancer chemoprevention: A trans pharmacological perspective (scoping review)

Traditional medicine herbs as natural product matrices in cancer chemoprevention: A trans pharmacological perspective (scoping review)

Anti-inflammatory, Anti-estrogenic, and Anti-implantation activity of Bergia suffruticosa (Delile) Fenzl

A Systematic Review on the Pharmacological Prospectives of <i>Vitex negundo</i> in <i>Ayurveda</i> Drug Research

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