Antifeedant activity of citrus limonoids against Colorado potato beetle: comparison of aglycones and glucosides

Murray, Kathleen D.; Hasegawa, Shin; Alford, A. R.

doi:10.1046/j.1570-7458.1999.00554.x

Cited by 12 publications

(15 citation statements)

References 18 publications

(20 reference statements)

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“…Two sesquiterpenes isolated from Senecio palmensis displayed antifeedant activity against CPB adults and larvae at 100 mg/cm 2 (Gonzalez- Coloma et al 1995). Citrus limonoids significantly reduced CPB larval feeding at concentrations > 10 mg/disc (Murray et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Leaf surface compounds of the potato (Solanum tuberosum) and their influence on Colorado potato beetle (Leptinotarsa decemlineata) feeding

Szafranek

Synak

Waligora³

et al. 2008

Chemoecology

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The sampling behavior of the Colorado potato beetle Leptinotarsa decemlineata Say (CPB) involves examination of the surface of potato leaves. It has been suggested that leaf surface compounds (volatiles and cuticular waxes) may be involved in host-plant recognition, acceptance or discrimination. Here we report on the effect of leaf surface extracts of six Polish commercial potato varieties on CPB feeding. We tested the influence of potato leaf surface extracts on CPB adult and larval feeding, then separated the extracts with HPLC, and finally tested the effect of the HPLC-separated fractions on CPB feeding. The bioassays were performed using potato leaf discs deprived of their original surface compounds. Applied to test discs at concentrations ten times higher than natural (10 leaf area equivalent), the extracts deterred CPB adults and larvae from feeding. HPLC-separated fractions composed of alkanes, sesquiterpene hydrocarbons, wax esters, benzoic acid esters, fatty acid methyl, ethyl, isopropyl and phenylethyl esters, aldehydes, ketones, methyl ketones, fatty acids, primary alcohols, b-amyrin and sterols did not affect adult CPB feeding. Similarly, alkanes, sesquiterpene hydrocarbons, wax esters, methyl ketones, sesquiterpene alcohols and secondary alcohols had no effect on larval CPB feeding. The sterol fraction (cholesterol, b-sitosterol and stigmasterol) acted as a phagostimulant to CPB larvae. We isolated a fraction demonstrating a phagodeterrent effect on CPB adults and larvae. The qualitative composition of the deterrent fraction was quite similar in all potato extracts, but there were quantitative differences between the varieties. Much further work is needed to identify the compounds that can produce the deterrent effect.

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Section: Discussionmentioning

confidence: 99%

Leaf surface compounds of the potato (Solanum tuberosum) and their influence on Colorado potato beetle (Leptinotarsa decemlineata) feeding

Szafranek

Synak

Waligora³

et al. 2008

Chemoecology

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“…Phytochemical materials produced by plants can demonstrate a range of toxicity, antifeedant or repellent effects on insects (Dev & Koul 1997;Perry et al 1998;Murray et al 1999;Khambay & Jewess 2000). Some of these products have been registered for use as pest controls, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, plant extracts may be good pest control candidates, particularly those from plants that have been used ingeniously for using pest control and other purposes in many area of the world (Duke 1990;Dev & Koul 1997). There have been growing efforts to discover new plant compounds that have insecticidal properties for developing plant derived insecticides (Murray et al 1999;Scott et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Contact and residual toxicities of 30 plant extracts to Colorado potato beetle larvae

Gökçe

Whalon

Çam

et al. 2007

Archives Of Phytopathology And Plant Protection

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Contact and residual toxicities of 30 plant extracts were investigated on third instar larvae of Colorado potato beetle, Leptinotarsa decemlineata. The plant samples were collected during the spring and summer of 2002 and were dried and ground. The plant samples were treated with methanol and the residue was eluted with distilled water containing 10% acetone, resulting in plant extracts. In contact bioassays, the beetle larvae were treated with 40% (w/w) plant extract using a Potter Spray Tower. The insects were incubated at 28+28C under a 16 h: 8 h photo regime and the mortality was recorded at 24 h intervals for 7 days. The plant extracts exhibited varying toxicity to the larvae ranging from 0 -91% after 24 h incubation and Artemisia vulgaris, Hedera helix, Humulus lupulus, Lolium temulentum, Rubia tinctoria, Salvia officinalis, Sambucus nigra, Urtica dioica, Verbascum songaricum, and Xanthium strumarium extracts resulted in significantly higher mortality than the control. Generally, prolonged incubation time did not result in an increase in mortality. After 48 h of incubation, 10 plant extracts yielded a significant mortality and H. lupulus extract, the most toxic extract among those tested, caused 99% mortality which is similar the mortality caused by the chemical standard, imidacloprid. In residual assays, potato leaflets were treated with 20% (w/w) plant-extract concentrations using a Potter Spray Tower. Third instar larvae were added to a glass jar to which treated leaflets were transferred before incubation at the temperature and photo regime described above. Mortality was recorded at 24 h intervals for 7 days. Five plant extracts, H. lupulus, L. temulentum, Reseda lutea and Solanum nigrum, induced significantly higher mortalities compared with controls. Chenopodium album extract was the most toxic, killing 34.9% of exposed larvae, followed by L. temulentum, H. lupulus and S. nigrum after 72 h incubation. The results exhibited that certain plant extracts were toxic to the beetle larvae and may have potential for controlling this destructive pest under field conditions.

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“…It means that this oil contains any other limonoid like obacunane, deacetylnomilin, ichangensin, etc. which became the main contributors of lethality (Klocke and Kubo 1982;Alford et al 1987;Murray et al 1999). As the project was tested with only two commercially available standards of limonoids (limonin and nomilin) which are in abundance, however, if more standards were available, these results would have been more comprehensive.…”

Section: Resultsmentioning

confidence: 99%

Mosquito larvicidal activity of citrus limonoids against Aedes albopictus

2011

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Citrus limonoids, nomilin and limonin, were used for larvicidal assay against Aedes albopictus utilizing WHO methodology. LC(50s) were 305.83, 176.08, and 136.07 μM for nomilin and 850.09, 600.72, and 407.09 μM for limonin after 24, 48, and 72 h, respectively. LT(50) assays exhibited that Savage citrange oil was the best at all concentrations (400, 500, 600, and 700 ppm) while Fairchild and Cassa grande were the weakest oils at 400 ppm, but at 500, 600, and 700 ppm, Carrizo citrange remained at the bottom with highest LT(50) values. Results exhibited that nomilin was more toxic than limonin and therefore provided a clear indication that limonoids in sample oils influenced the potential of respective oil. Out of the 10 tested citrus seed oils, Savage citrange (Citrus sinensis) comprised the maximum amount of limonin (2823.59 μg/ml) followed by grapefruit, Sacaton citrumelo, and Jaffa. When this oil (Savage citrange) was evaluated for bioassay against larvae of Ae. albopictus, it reflected complete dominance (LC(50) and LT(50)) as compared to rest of the oils. Although Jaffa (Citrus paradisi) was found to contain nomilin and limonin, it was found less effective as compared to Savage citrange. The oils from Minneola and Chinese lime did not contain limonin and nomilin, and were therefore weak in terms of LC(50) values. Presence of limonin and nomilin in plant products is therefore a significant indicator of the pest control that needs to be exploited in other plants as well.

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Antifeedant activity of citrus limonoids against Colorado potato beetle: comparison of aglycones and glucosides

Cited by 12 publications

References 18 publications

Leaf surface compounds of the potato (Solanum tuberosum) and their influence on Colorado potato beetle (Leptinotarsa decemlineata) feeding

Leaf surface compounds of the potato (Solanum tuberosum) and their influence on Colorado potato beetle (Leptinotarsa decemlineata) feeding

Contact and residual toxicities of 30 plant extracts to Colorado potato beetle larvae

Mosquito larvicidal activity of citrus limonoids against Aedes albopictus

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