Antifibrosis effects of total glucosides of Danggui–Buxue–Tang in a rat model of bleomycin-induced pulmonary fibrosis

Gao, Jian; Huang, Yan; Li, Ping; Xu, Donghui; Li, Jun; Liu, Yong; Huang, Zhenchi; Wu, Qiang; Xu, Sheng

doi:10.1016/j.jep.2011.03.013

Cited by 31 publications

(31 citation statements)

References 23 publications

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“…Zhao et al (48) have also demonstrated that Smad3 deficiency attenuates bleomycin-induced PF in mice. In the present study, OM treatment effectively inhibited the expression of TGF-β1 and the BLM-activated Smad2 and Smad3, which is consistent with previous studies showing that amelioration of fibrosis is often associated with the inhibition of the TGF-β/Smad signaling pathway (49)(50)(51)(52). Moreover, our results are also in agreement with the findings of Wu et al (15) who found that OM has antifibrotic effects in the liver via the inhibition of the TGF-β/Smad pathway (15).…”

Section: Discussionsupporting

confidence: 93%

Oxymatrine attenuates bleomycin-induced pulmonary fibrosis in mice via the inhibition of inducible nitric oxide synthase expression and the TGF-β/Smad signaling pathway

Liu

Lü

et al. 2012

Int J Mol Med

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Abstract. Oxymatrine (OM) is an alkaloid extracted from the Chinese herb Sophora flavescens Ait. with a variety of pharmacological activities. The aim of this study was to investigate the preventive effects of OM on bleomycin (BLM)-induced pulmonary fibrosis (PF) and to further explore the underlying mechanisms. C57BL/6 mice were randomly assigned to five groups: the saline sham group; the BLM group, in which mice were endotracheally instilled with BLM (3.0 mg/kg); and the BLM plus OM groups, in which OM was given to mice daily (10, 20 or 40 mg/kg) one day after BLM instillation for 21 days. The bronchoalveolar lavage fluid (BALF) and lung tissues were collected at 15 and 22 days post BLM administration, respectively. Lung tissues were stained with hematoxylin and eosin (H&E) for histological evaluation. Levels of tumor necrosis factor (TNF)-α, interleukin-6 (IL-6) and nitric oxide (NO) in mouse BALF were measured, as well as myeloperoxidase (MPO) activity and malondialdehyde (MDA) content in lung homogenates. The inducible nitric oxide synthase (iNOS) expression in the lung tissues was determined by immunohistochemical staining, quantitative real-time PCR and western blot analysis. Moreover, the expression of transforming growth factor (TGF)-β1, Smad2, Smad3, p-Smad2 and p-Smad3 were also detected. We found that OM improved BLM-induced lung pathological changes, inhibited MPO activity and reduced MDA levels in a dose-dependent manner. OM also dose-dependently inhibited the release of TNF-α and IL-6, and decreased the expression of iNOS in lung tissues and thus prevented NO release in response to BLM challenge. In addition, OM decreased the expression of TGF-β1, p-Smad2 and p-Smad3, which are all important members of the TGF-β/ Smad signaling pathway. Our study provides evidence that OM significantly ameliorated BLM-induced PF in mice via the inhibition of iNOS expression and the TGF-β/Smad pathway.

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Section: Discussionsupporting

confidence: 93%

Oxymatrine attenuates bleomycin-induced pulmonary fibrosis in mice via the inhibition of inducible nitric oxide synthase expression and the TGF-β/Smad signaling pathway

Liu

Lü

et al. 2012

Int J Mol Med

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“…In the current study, we found that the substantial lung architecture was distorted including lung edema, infiltration of the tissue, alveoli with inflammatory cells and thickening of the alveolar wall after bleomycin administration [27,28]. Meanwhile, the collagen expression, HYP content, and the levels of LN and HA were dramatically increased in PF model, indicating that ECM production was abnormally elevated.…”

Section: Effect Of Ypf-g On Hyp Content In Lung Tissuesmentioning

confidence: 56%

“…A large number of therapeutic interventions have been attempted to reverse established fibrosis, such as anticoagulation, endothelin receptor antagonists and prednisone, whereas lack of efficacy or increased mortality was demonstrated [26]. In recent years, it has been increasingly recognized and supported that extract from traditional Chinese medicine has the prophylactic and therapeutic effects on PF [20,27]. Here, we developed a PF model to investigate the potential protective effect and mechanism of YPF-G on rats, with a focus on HMGB1 activation and EMT.…”

Section: Effect Of Ypf-g On Hyp Content In Lung Tissuesmentioning

confidence: 99%

Total glycosides of Yupingfeng protects against bleomycin-induced pulmonary fibrosis in rats associated with reduced high mobility group box 1 activation and epithelial–mesenchymal transition

Cui

et al. 2015

Inflamm. Res.

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“…1. These ensured the chemical consistencies among different preparations (Yang et al, 2009;Gao et al, 2008Gao et al, , 2011.…”

Section: Introductionmentioning

confidence: 99%

“…Several previous studies showed DBT can promote hematopoiesis, stimulate cardiovascular circulation, prevent osteoporosis and posses anti-oxidation activity (Gao et al, 2011;Mak et al, 2006;Gao et al, 2007). However, there is a lack of scientific studies on its potential pharmacological properties.…”

Section: Introductionmentioning

confidence: 99%

Effect of Danggui Buxue Tang on immune-mediated aplastic anemia bone marrow proliferation mice

Xj¹,

Huang²,

Du³

et al. 2014

Phytomedicine

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Antifibrosis effects of total glucosides of Danggui–Buxue–Tang in a rat model of bleomycin-induced pulmonary fibrosis

Cited by 31 publications

References 23 publications

Oxymatrine attenuates bleomycin-induced pulmonary fibrosis in mice via the inhibition of inducible nitric oxide synthase expression and the TGF-β/Smad signaling pathway

Oxymatrine attenuates bleomycin-induced pulmonary fibrosis in mice via the inhibition of inducible nitric oxide synthase expression and the TGF-β/Smad signaling pathway

Total glycosides of Yupingfeng protects against bleomycin-induced pulmonary fibrosis in rats associated with reduced high mobility group box 1 activation and epithelial–mesenchymal transition

Effect of Danggui Buxue Tang on immune-mediated aplastic anemia bone marrow proliferation mice

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