Antifibrotic Effects of Immobilized Hyaluronidase in Repeated Bleomycin-Induced Lesions of the Alveolar Epithelium

Abstract

Antifibrotic activity of testicular hyaluronidase, immobilized on polyethylenoxide and obtained by electron beam synthesis, was studied on the model of bleomycin injuries to the alveolar epithelium (irreversible pneumofibrosis) in C57Bl/6 mice and compared to the effect of testicular hyaluronidase. Intranasal therapy with immobilized and testicular hyaluronidases prevented the deposition of fibrotic mass in the parenchyma of "bleomycin" lungs. The effect of immobilized hyaluronidase was more pronounced than that of testicular hyaluronidase. The studied compounds were virtually inessential for infiltration of the alveolar interstitium and alveolar tracts by lymphocytes, macrophages, neutrophils, and plasma cells. Unchanged histoarchitectonics of bleomycin-damaged lungs in immobilized hyaluronidase therapy was due to suppression of the progenitor fibroblast cells (CD45(-)).

A nonsynonymous polymorphism (rs117179004, T392M) of hyaluronidase 1 (HYAL1) is associated with increased risk of idiopathic pulmonary fibrosis in Southern Han Chinese

“…8 Intranasal immobilized hyaluronidase prevented connective tissue growth in the lungs exposed to bleomycin, indicating antifibrotic effect of hyaluronidase. 29 Alternative mRNA splicing exhibited different cellular expression of enzymatically active of hyaluronidase and may explain the elevated hyaluronidase levels in tumor bladder/prostate cancer. 30 Hence, we suppose this variant may affect the development of IPF by potentially altering gene expression or weakening enzyme activity.…”