Antifilarial activity of Butea monosperma L. leaves extracts against Setaria cervi

Abstract

Background: Usage of herbal drugs in traditional medicine is quite well known but largely empirical. Hence the present study was designed to screen the in vitro antifilarial effect of Butea monosperma L. plant leaves against Setaria cervi. Methods: Antifilarial activity of Methanol/Hexane-ethanol extracts of Butea monosperma L. (leaves) and Ciprofloxacin was explored against adult of Setaria cervi after incubation for 24 hrs with concentration range of 0.25 to 20 mg/ml for possible antifilarial effect by comparing with suitable control, in terms of motility inhibition assay and MTT reduction assay. Results: Butea monosperma L. plant leaves showed significant antifillarial activity against adult as compared to controls whereas activity demonstrated by Ciprofloxacin was comparatively less significant. Inhibitory concentrations (IC50) for the plant extracts with significant antifilarial activity against Setaria cervi adult in vitro system have been derived to be 1.25, 3.6 and 7.5 mg/ml Methanol, Hexane-ethanol extracts and Ciprofloxacin respectively. Conclusion: The present research investigation proved to be an additional frame in recording the plant extract's antifilarial activity. Methanol and Hexane-ethanol extracts of Butea monosperma L. plant leaves which shown significant antifilarial activity. The present research data highlights the importance of further depth research in this area.

In vitro evaluation of antifilarial effect of Azadirachta indica leaves extract in different solvents on the microfilariae of Setaria cervi

“…Highest mortality rate of 100 and 90 % was caused by methanol and ethanol extract at 200 lg/ml concentration after 135 min, indicating that the mortality rate of methanol and ethanol are highest and highly effective against the microfilariae of S. cervi. In contrast in earlier study on Butea monosperma inhibitory effect on the microfilariae of S. cervi was observed at the higher concentration of 1.25 mg/ml methanol and 3.6 mg/ml hexane-ethanol extracts when incubated for 24 h (Deshmukh et al 2014). Anthelmintic effect of pure A. indica leaves extract on L3 larvae of Haemonchus contortus was maximum at 1000 lg concentration (Radhakrishnan et al 2007).…”

Review on Medicinal Importance of Butea monosperma Lam. (Taub)

“…The hexane ethanolic and methanolic excerpt of BM leaves have shown remarkable anti-filarial activity about motility embarrassment evaluation and MTT reduction evaluation. It was also seen that maximum inhibition was reported at 5 mgml-1 concentration with 5 h incubation period 52 .…”

Programmed cell death pathways as targets for developing antifilarial drugs: Lessons from the recent findings

“…According to Sahare et al, 148 the root extract of V. negundo contains saponin which is apoptotic in nature. Butea monosperma is well known for its medicinal value as used to treat various ailments in ‘Ayurveda’, commonly called the flame of the forest or palasa have antimicrofilariae activity against B. malayi 149 . Evidence of lipid peroxidation in the B. monosperma leave extract used to treat microfilaria can induce oxidative stress along with the presence of saponin in the extract has validated the relation between oxidative stress with apoptosis 149,150 .…”

“…Butea monosperma is well known for its medicinal value as used to treat various ailments in ‘Ayurveda’, commonly called the flame of the forest or palasa have antimicrofilariae activity against B. malayi . 149 Evidence of lipid peroxidation in the B. monosperma leave extract used to treat microfilaria can induce oxidative stress along with the presence of saponin in the extract has validated the relation between oxidative stress with apoptosis. 149 , 150 Cajanus scarabaeoides commonly known as showy pigeonpea has diverse therapeutic value in treating anaemia, smallpox, dysentery, cholera, gonorrhoea and rinderpest as an ethnomedicine.…”

“… 149 Evidence of lipid peroxidation in the B. monosperma leave extract used to treat microfilaria can induce oxidative stress along with the presence of saponin in the extract has validated the relation between oxidative stress with apoptosis. 149 , 150 Cajanus scarabaeoides commonly known as showy pigeonpea has diverse therapeutic value in treating anaemia, smallpox, dysentery, cholera, gonorrhoea and rinderpest as an ethnomedicine. Among the chloroformic, ethanolic, ethyl acetate and petroleum etheric extraction, ethanolic extracts derived from the stem part of C. scrabaeoides are polyphenol enriched and show the most feasible effect when treated against oocytes, mf and adults S. cervi in activating ROS‐dependent CED pathway of apoptosis.…”