Antifungal activity and mechanism of tea polyphenols against Rhizopus stolonifer

Abstract

The mechanism of action might be attributed to direct damage of the mycelium and spore and indirect induction of defensive enzyme activities. TP has the potential to be developed as an alternative to control post-harvest disease of fruit caused by R. stolonifer.

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“…Del mismo modo, las infecciones por hongos han aumentado en los últimos años, debido a la resistencia de los fitopatógenos a los fungicidas, el cual tiene un impacto significativo en la economía debido a las pérdidas postcosecha causadas por varios hongos (Yang & Jiang, 2015). En la última década, hay un gran interés en el estudio de los compuestos naturales como sustitutos de fungicidas sintéticos y un gran número de estudios in vitro ha demostrado que los efectos antifúngicos de los extractos de plantas reducen la incidencia de patógenos postcosecha (Ramos-Guerrero et al, 2018).…”

“…In the same way, fungal infections have been increasing in the last years, due to the resistance of phytopathogens to fungicides, which has a significant impact on the economy due to the post-harvest losses caused by various fungi (Yang & Jiang, 2015). In the last decade, there is a great interest in the study of natural compounds as substitutes for synthetic fungicides and a large number of in vitro studies has demonstrated that the antifungal effects of plant extracts reduce the incidence of post-harvest pathogens (Ramos-Guerrero et al, 2018).…”

“…Therefore, it is highly probable that polyphenols, alkaloids and acetogenins are present in the fractions due to the solvents used. Yang & Jiang (2015) reported that there is a dose-dependent in polyphenolic concentration to inhibit mycelial growth and spore germination of R. stolonifer. The polyphenols caused morphological changes on the hyphae such as irregularly swollen appearance, increasing branching, wrinkles, entwining, collapse and breakage, while in the spores treated with tea polyphenols was observed swelling of germ tube tips, exfoliation of the surface layer and disorganization of cell organelles.…”

“…y tienen un amplio espectro de acción(Coria- Téllez et al, 2018). Por lo tanto, es altamente probable que contenga polifenoles, alcaloides y acetogeninas en las fracciones debido a los solventes utilizados Yang & Jiang (2015). reportaron que existe una dosis dependiente de la concentración polifenólica para inhibir el crecimiento micelial y la germinación de esporas de R. stolonifer.…”

Antifungal activity and mechanism of heat-transformed ginsenosides from notoginseng against Epidermophyton floccosum, Trichophyton rubrum, and Trichophyton mentagrophytes

“…The antifungal experiment was performed using the micro dilution method of Yang and Jiang. 30 Cell suspensions were diluted in sterile SAM to provide initial cell counts of about 10 8 colony-forming units per mL (CFU mL À1 ). Miconazole nitrate (2 mg) was dissolved in DMSO (2 mL) for the subsequent antifungal experiments as the positive control.…”