Antifungal Activity of a Neodymium-Doped Yttrium Aluminum Garnet 1,064-Nanometer Laser against Sporothrix globosa by Inducing Apoptosis and Pyroptosis via the NLRP3/Caspase-1 Signaling Pathway:
            <i>In Vitro</i>
            and
            <i>In Vivo</i>
            Study

Yan, Tianyi; Li, Fuqiu; Li, Jinran; Chen, Feng

doi:10.1128/spectrum.01364-21

Cited by 8 publications

(5 citation statements)

References 37 publications

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“…Therefore, the Bax and Bcl-2 protein level changes will aggravate cardiac injury and dysfunction induced by LPS, while relieving these changes might alleviate LPS-induced cardiac damage. Besides, the proteins of cleaved-Casepase-1 and cleaved-Casepase-3 also play a vital part in developing cardiac apoptosis [27]. In the current study, LPS triggered myocardial apoptosis, as evidenced by elevations in cleaved-Casepase-1, cleaved-Casepase-3, Bax protein levels, and decreased Bcl-2 protein level.…”

Section: Discussionsupporting

confidence: 57%

5-Methoxytryptophan pretreatment alleviates lipopolysaccharide-induced cardiac injury and dysfunction

Dong

2022

Appl Biol Chem

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Reducing inflammation is a promising therapeutic approach for sepsis-induced cardiomyopathy (SIC). The 5-Methoxytryptophan (5-MTP) is a tryptophan metabolite that demonstrates anti-inflammatory, anti-fibrosis, anti-tumorigenesis, and anti-senescence features. Current investigations aimed to assess the 5-MTP pretreatment impacts on lipopolysaccharide (LPS)-induced cardiac injury and dysfunction. For in vivo studies, the mice were categorized randomly into four groups: control, LPS, LPS+5-MTP (25 mg/kg) and LPS+5-MTP (50 mg/kg). The mice in the LPS+5-MTP groups were given 5-MTP intraperitoneally once a day for 7 days. LPS (10 mg/kg) was then administered intraperitoneally for 24 h. Echocardiography, cardiac injury biomarkers, and H & E staining evaluated heart anatomy and function. The findings indicate that 5-MTP pretreatment significantly reduced LPS-induced heart dysfunction and morphological alterations. Western blot assay was used for investigating molecular mechanisms. After LPS stimulation, the pro-inflammatory cytokines (IL-1β, IL-6, TNF-α and NLRP3) protein levels increased while anti-inflammatory cytokine (IL-10) decreased; however, 5-MTP pretreatment mitigated this response by suppressing the stimulation of the NF-κB signaling pathway. Furthermore, 5-MTP administration reduced LPS-induced cardiac apoptosis, as demonstrated by increased protein levels of cleaved-Casepase-1, cleaved-Casepase-3 and Bax, and decreased protein level of Bcl-2 after LPS stimulation, whereas LPS-induced cardiac apoptosis was reversed by 5-MTP pretreatment. In vitro, 5-MTP pretreatment had a similar cardioprotective effect on cultured cardiac fibroblasts challenged with LPS. In conclusion, 5-MTP pretreatment can reduce LPS-induced cardiac inflammation and apoptosis, implying that 5-MTP is a possible therapeutic option for SIC.

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Section: Discussionsupporting

confidence: 57%

5-Methoxytryptophan pretreatment alleviates lipopolysaccharide-induced cardiac injury and dysfunction

Dong

2022

Appl Biol Chem

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“…The study by Yan et al, 2021 also demonstrated the laser's effect on S. globosa cells, inducing apoptosis and pyroptosis via NLRP3/Caspase-1, activated by the TH1/TH17 cellular response. This type of treatment aligns with the clinical presentation of the treated feline's ear lesion, as it is believed that laser therapy assisted the immune system in combating the lesion's microorganisms [ 17 ].…”

Section: Discussionmentioning

confidence: 91%

Beyond conventional approaches: Enhancing photodynamic therapy for refractory feline sporotrichosis caused by Sporothrix brasiliensis

Mesquita Ramos,

de Souza Rabello,

Ribeiro da Silva

et al. 2024

Medical Mycology Case Reports

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“…PRRs expressed on the surfaces of epithelial cells and dendritic cells can help with the recognition of pathogens. PRRs, including Mannose receptors, Dectin-1, toll-like receptors, and NOD-like receptors, have been reported to be involved in the immune process 34 , 35 . The innate immunity represents the host's first line of defense against pathogens 36 .…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that the NOD-like receptor protein 3 (NLRP3) inflammasome plays a critical role against S. globosa infection. Yan et al 35 treated the footpads of S. globosa -infected mice with Nd:YAG 1064 nm laser irradiation, and concluded that the local NLRP3/caspase-1 pyroptosis pathway was activated and promoted Th1/Th17 cell immunity, and the critical effector of pyroptosis caspase-3 was upregulated after the laser treatment. Gonçalves et al 52 found that NLRP3, ASC, or caspase-1 knockout mice were more susceptible to S. schenckii infection than wild-type mice, suggesting that NLRP3-triggered-inflammatory responses contributed to host protection against infection.…”

Section: Discussionmentioning

confidence: 99%

Whole transcriptome sequencing for revealing the pathogenesis of sporotrichosis caused by Sporothrix globosa

Liu,

Li,

Zhang

et al. 2024

Sci Rep

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This study aimed to investigate the molecular mechanism of sporotrichosis and identify possible novel therapeutic targets. Total RNA was extracted from skin lesion samples from sporotrichosis patients and used to construct a long-chain RNA transcriptome library and miRNA transcriptome library for whole transcriptome sequencing. The differentially expressed genes (DEGs) between the groups were identified, and then Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Gene Set Enrichment Analysis enrichment analyses were performed based on the DEGs. An lncRNA-miRNA-mRNA ceRNA network was constructed. The expressions of JAK/STAT pathway-related proteins were detected in the patient and control tissues using RT-qPCR and Western blot analysis. Enrichment analysis showed that the DEGs were mainly enriched in various infectious diseases and immune response-related signaling pathways. Competing endogenous RNA network analysis was performed and identified the hub lncRNAs, miRNAs, and mRNAs. Compared with the control group, the mRNA expressions of SOCS3, IL-6, and JAK3 were significantly upregulated, while the expression of STAT3 did not change significantly. Also, the protein expressions of SOCS3, IL-6, JAK3, and STAT3, as well as phosphorylated JAK3 and STAT3, were significantly upregulated. We identified 671 lncRNA DEGs, 3281 mRNA DEGs, and 214 miRNA DEGs to be involved in Sporothrix globosa infection. The study findings suggest that the JAK/STAT pathway may be a therapeutic target for sporotrichosis.

show abstract

Antifungal Activity of a Neodymium-Doped Yttrium Aluminum Garnet 1,064-Nanometer Laser against Sporothrix globosa by Inducing Apoptosis and Pyroptosis via the NLRP3/Caspase-1 Signaling Pathway: In Vitro and In Vivo Study

Cited by 8 publications

References 37 publications

5-Methoxytryptophan pretreatment alleviates lipopolysaccharide-induced cardiac injury and dysfunction

5-Methoxytryptophan pretreatment alleviates lipopolysaccharide-induced cardiac injury and dysfunction

Beyond conventional approaches: Enhancing photodynamic therapy for refractory feline sporotrichosis caused by Sporothrix brasiliensis

Whole transcriptome sequencing for revealing the pathogenesis of sporotrichosis caused by Sporothrix globosa

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