Antifungal Activity of Aerial Parts of Cenchrus pennisetiformis Against Fusarium oxysporum f. sp. lycopersici

Khurshid, Saba; Javaid, Arshad; Shoaib, Amna; Javed, Sidra; Qaisar, Uzma

doi:10.1590/s0100-83582018360100023

Cited by 13 publications

(7 citation statements)

References 12 publications

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“…Afterwards, the residual extract was sequentially partitioned with chloroform) (400 mL) and ethyl acetate) (400 mL). All the fractions in organic solvents were evaporated on a rotary evaporator to gain 30 g of n-hexane) fraction, 10 g of (chloroform) fraction and 2 g of (ethyl acetate fraction (Khurshid et al, 2018).…”

Section: Fractionation Of Methanolic Leaf Extractmentioning

confidence: 99%

Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici

2019

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The present study was performed to evaluate antifungal activity and GC-MS analysis of leaf extract of Chenopodium mural against Fusarium oxysporum f. sp. lycopersici (FOL), a highly problematic soil-borne pathogen of tomato. Dried leaves of C. murale were extracted with methanol for 2 weeks and after evaporating the solvent on a rotary evaporator, antifungal bioassay was carried out against FOL. All concentrations (1 to 5%) significantly reduced FOL biomass by 14-45%. The remaining methanolic extract was fractioned with n-hexane, chloroform and ethyl acetate and all these fractions were assayed for their antifungal potential. A 200 mg mL-1 concentration of various sub-fractions reduced fungal biomass significantly by 94-98% over control. All the sub-fractions were subjected to GC-MS analysis that revealed presence of 32 compounds in n-hexane, 2 compounds in chloroform and 13 compounds in ethyl acetate sub-fraction. The predominant compounds in n-hexane sub-fraction were hexadecanoic acid, methyl ester (14.64%), methyl linolenate (16.61%) and g-sitosterol (13.53%). In chloroform sub-fraction, bis (2-ethylhexyl) phthalate (92.31% and in ethyl-acetate sub-fraction, ethyl butyrate (19.57%), dihexyl phthalate (11.19%) and dioctyl phthalate (12.16%) were present in higher concentration.

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Section: Fractionation Of Methanolic Leaf Extractmentioning

confidence: 99%

Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici

2019

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“…However, numerous fungicides available in the market are not only toxic but also pollute the environment (Anggriani et al, 2015) and create resistance in fungal pathogens (Kim et al, 2007). A likely alternative to resolve such problems is the use of natural resources, including microorganisms and products from higher plants (De Corato et al, 2014;Ali et al, 2017;Khurshid et al, 2018). Protecting plants against fungal pathogens using such alternatives may also reduce development of resistance in the pathogens (Trigui et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Management of Charcoal Rot of Mungbean by Two Trichoderma Species and Dry Biomass of Coronopus didymus

2018

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A pot experiment was carried out to check the effect of Coronopus didymus (L.) Sm. dry biomass application (1%, 2% and 3% w/w) and two species of Trichoderma (T. viride and T. aureoviride) on growth and physiology of mungbean [Vigna radiata (L.) WIlczek] under biotic stress of Macrophomina phaseolina (Tassi) Goid. Inoculation of M. phaseolina (positive control) reduced plant survival, shoot and root length as well as plant dry biomass by 22%, 52%, 61% and 64%, respectively, over the negative control (without any amendment). There was 100% plant survival in treatments with T. aureoviride alone or in combination with 1% and 2% C. didymus biomass. Likewise, T. viride in combination with 2% biomass also showed 100% plant survival. Application of 3% C. didymus biomass had a pronounced effect on crop growth resulting in 101%, 233% and 342% increase in shoot length, root length and plant biomass, respectively, over the positive control. Sole inoculation of either of the two Trichoderma spp. significantly enhanced various plant growth parameters over the positive control. In general, in combination with 2% biomass of C. didymus, both Trichodermaspp. proved to be the best choice for improving mungbean biomass under stress of M. phaseolina. Activity of denfense related enzymes viz. peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) was generally higher in treatments in which 1% C. didymus biomass was applied either alone or combined with Trichoderma spp. in M. phaseolina inoculated soil.

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“…Thereafter, materials were filtered with muslin cloth followed by 3 through filter papers to get the extracts. Methanolic extracts were subjected to rotary evaporation to get crude leaf, stem and fruit extracts of 22 g, 18 g and 14 g, respectively (Khurshid et al, 2018).…”

Section: Bioassays With Methanolic Extractsmentioning

confidence: 99%

Bioactive Constituents of Shoot Extracts of Sisymbrium irio L. Against Fusarium oxysporum f. sp. cepae

2020

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The present study was carried out to check the antifungal potential of Sisymbrium irio L. shoot extract against Fusarium oxysporum f. sp. cepae (FOC). In preliminary bioassays, different concentrations (1 to 5%) of leaf, stem and fruit extracts were evaluated against FOC. All the extracts were effective against the pathogen. However, the leaf extract was found the most effective causing 25-41% decrease in FOC biomass. The fractionation of methanolic leaf extract was done by two organic solvents namely n-hexane and chloroform. Different concentrations (1.56 to 200 mg mL-1) of these fractions were tested against FOC. The n-hexane and chloroform fractions showed inhibitory activity against the pathogen and resulted in 77-93% and 80-96% reduction in biomass of FOC, respectively. GC-MS analysis showed the presence of 24 compounds in n-hexane and 4 compounds in chloroform fraction. In n-hexane fraction, β-sitosterol (18.64%) was the most abundant compound followed by orotic acid, bis(tert-butyldimethylsilyl)-, tert-butyldimethylsilyl ester (12.18%), 10-octadecenoic acid, methyl ester (7.90%) and 1,2-benzenedicarboxylic acid, diisooctyl ester (6.05%). Major compounds identified in chloroform fraction were 1,3-benzenedicarboxylic acid, bis(2-ethylhexyl) ester (50.82%) and di-n-octyl phthalate (33.00%). This study concludes that n-hexane and chloroform fractions of methanolic leaf extract of S. irio contain potent antifungal constituents for the management of FOC.

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Antifungal Activity of Aerial Parts of Cenchrus pennisetiformis Against Fusarium oxysporum f. sp. lycopersici

Cited by 13 publications

References 12 publications

Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici

Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici

Management of Charcoal Rot of Mungbean by Two Trichoderma Species and Dry Biomass of Coronopus didymus

Bioactive Constituents of Shoot Extracts of Sisymbrium irio L. Against Fusarium oxysporum f. sp. cepae

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