Antifungal activity of biopolymers containing natamycin and rosemary extract against Aspergillus niger and Penicillium roquefortii

Abstract

SummaryAntimicrobial agent-releasing films have been proposed as an effective way of inhibiting chiefly surface spoilage of food products. Antifungal activities of natamycin (NA), rosemary extract (RE) and NA + RE were tested against Aspergillus niger and Penicillium roquefortii with agar disc diffusion assay. NA, RE and NA + RE were also included into biopolymers made from gluten and methyl cellulose. Minimum inhibitory concentrations (MIC) of NA in both films were 2 and 1 mg NA per 10 g film solution against A. niger and P. roquefortii, respectively. RE did not show any inhibitory antifungal activity alone. Although NA incorporated into both films at a concentration of 1.5 mg NA per 10 g film solution was not effective against A. niger, combination of NA at the same concentration with RE in the films inhibited the growth of this mould. NA in solution or in biopolymers is very effective in inhibiting the growth of selected organisms, and RE acted synergistically with NA to prevent the growth of A. niger when incorporated into both films.

Physical and antifungal properties of hydroxypropylmethylcellulose based films containing propolis as affected by moisture content

“…The inhibitory effect of HPMC films enriched with propolis on A. niger is shown in Table 3. Pure HPMC films were ineffective at reducing A. niger growth, as no significant differences exist between fungal growth on control and HPMC PDA plates, coinciding with previous studies with methylcellulose, another derivative of cellulose (Türe, Eroglu, Soyer, & Özen, 2008). Propolis incorporation into HPMC films significantly (p < 0.05) reduced fungal population of A. niger which agrees with previous studies carried out by Ozcan (1999) and Soylu et al (2008).…”

Antimicrobial compression-moulded wheat gluten films containing potassium sorbate

“…They have also been considered as carriers for antimicrobial agents (Gucbilmez, Yemenicioglu, & Arslanoglu, 2007;Pintado, Ferreira, & Sousa, 2009;Ture, Eroglu, Soyer, & Ozen, 2008). One of the most frequently studied proteins in edible films is wheat gluten (WG).…”

Suppression of mould growth on dry sausages

“…A modern method is the use of inside coated packaging materials (Hanušová et al 2010). Another way to apply antifungal agents is to incorporate them into biopolymer films, especially natamycin with rosemary extract (Ture et al 2008).…”